NEFM

Gene Symbol NEFM
Entrez Gene 4741
Alt Symbol NEF3, NF-M, NFM
Species Human
Gene Type protein-coding
Description neurofilament, medium polypeptide
Other Description 160 kDa neurofilament protein|neurofilament 3|neurofilament medium polypeptide|neurofilament triplet M protein|neurofilament, medium polypeptide 150kDa|neurofilament-3 (150 kD medium)
Swissprots Q4QRK6 E9PBF7 B4DGN2 P07197
Accessions CAA68276 EAW63602 P07197 AF181990 AAF00492 AK294681 BAG57843 AK296226 BAG58949 BC002421 BC071752 BC096757 AAH96757 BI496345 BP247321 BP311096 DC318935 DC332005 DC352660 EF560736 ABQ59046 EF560737 ABQ59047 GQ129242 ACT64376 GQ129243 ACT64377 NM_001105541 NP_001099011 NM_005382 NP_005373
Function Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Top Pathways Amyotrophic lateral sclerosis (ALS)

Neurofilament-M (RMO 14.9) Mouse mAb - 2838 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Western blot analysis of extracts from rat brain, untreated or treated with lambda phosphatase, using Neurofilament-M (RMO 14.9) Mouse mAb.

NF-M (E-9) - sc-398532 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NF-M (E-9): sc-398532. Western blot analysis of NF-M expression in EOC 20 (A), HeLa (B), MCF7 (C) and SHP-77 (D) whole cell lysates.

NF-M/H (RMdO-20) - sc-32273 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NF-M/H (RMdO-20): sc-32273. Western blot analysis of neurofilament 160/200 expression in rat brain tissue extract.

NF-M (1A2) - sc-20013 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NF-M (1A2): sc-20013. Immunofluorescence staining of methanol-fixed SH-SY5Y cells showing cytoplasmic localization.

NF-M (G-20) - sc-16143 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NF-M (G-20): sc-16143. Western blot analysis of NF-M expression in mouse brain.

NF-M (H-60) - sc-25653 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NF-M (H-60): sc-25653. Western blot analysis of NF-M expression in HeLa (A) and EOC 20 (B) whole cell lysates.

NF-M (NF-09) - sc-51683 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
NF-M (NF-09): sc-51683. Western blot analysis of NF-M expression in rat brain (A) and mouse brain (B) tissue extracts.

NF-M (2Q168) - sc-71688 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µl ascites
Host Mouse
Clonality Monoclonal
NF-M (2Q168): sc-71688. Western blot analysis of NF-M expression in non-transfected: sc-110760 (A) and mouse NF-M transfected: sc-179000 (B) 293 whole cell lysates.

NF-M (3H11) - sc-58561 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µl ascites
Host Mouse
Clonality Monoclonal
NF-M (3H11): sc-58561. Immunofluorescence staining of methanol-fixed adult neural cells cells showing neurofilament localization.

p-NF-M (RNF403) - sc-32726 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p-NF-M (RNF403): sc-32726. Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing neuronal cystoskeleton localization.

p-NF-M (RNF406) - sc-32727 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p-NF-M (RNF406): sc-32727. Western blot analysis of NF-M phosphorylation in normal (A) and phospho-protein enriched (B) rat brain tissue extracts.

Anti-140 kD + 160 kD Neurofilament antibody - ab134980 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-140 kD + 160 kD Neurofilament antibody (ab134980) at 1/500 dilution + extracts from MCF7 cells at 30 µg

Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] - ab68142 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR580(2)Y
Immunohistochemical analysis of paraffin-embedded human brain tissue using ab68142 at a dilution of 1/100 - 1/250

Anti-160 kD Neurofilament Medium antibody - ab39371 from Abcam

Prices $378.00
Sizes 100 µl
Host Chicken
Clonality Polyclonal
Ab39371 staining Neurofilament in neonatal rat cortical neurons cultured for 14-21 days.

Anti-160 kD Neurofilament Medium antibody - ab134458 from Abcam

Prices $378.00
Sizes 200 µl
Host Chicken
Clonality Polyclonal
Immunofluorescent analysis of dissociated culture of adult rat brain labelling 160 kD Neurofilament Medium with ab134458 at 1/2000 dilution (green staining). Neurites are stained in a punctate fashion, corresponding to neuritic varicosities. As a control, rabbit GFAP antibodies were used to costain astrocytes, producing a red fluorescence. Blue nuclei demonstrate DAPI DNA staining.

Anti-160 kD Neurofilament Medium antibody [3H11] - ab7256 from Abcam

Prices $379.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 3H11
Lane 1 : Coomassie Blue stainLane 2 : Heavy neurofilament antibodyLane 3 : Anti-160 kD Neurofilament Medium antibody [3H11] (ab7256)Lane 4 : Anti-68kDa Neurofilament antibody [DA2] (ab7255)Lane 5 : alpha Internexin antibodyLane 6 : GFAP antibodyLane 1 : Cytoskeletal homogenates of rat spinal cordLane 2 : Cytoskeletal homogenates of rat spinal cordLane 3 : Cytoskeletal homogenates of rat spinal cordLane 4 : Cytoskeletal homogenates of rat spinal cordLane 5 : Cytoskeletal homogenates of rat spinal cordLane 6 : Cytoskeletal homogenates of rat spinal cord

Anti-160 kD Neurofilament Medium antibody [EP2460] - ab92539 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2460
All lanes : Anti-160 kD Neurofilament Medium antibody [EP2460] (ab92539) at 1/100000 dilutionLane 1 : 293T cell lysateLane 2 : SH-SY5Y cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Anti-160 kD Neurofilament Medium antibody [RMO44] - ab50284 from Abcam

Prices $388.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone RMO44
ab50284 staining 160 kD Neurofilament Medium in 16 µm thick sections of Apteronotus leptoryhnchus brain tissue by Immunohistochemistry (Frozen sections).Tissue was fixed with 2% paraformaldehyde, permeabilized using 0.3% Triton X-100, blocked with 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS and then incubated with ab50284 at a 1/200 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 488-conjugated goat anti-mouse polyclonal used at a 1/200 dilution.See Abreview

Anti-200kDa + 160kDa Neurofilament (phospho ) antibody [SMI 34] - ab24571 from Abcam

Host Mouse
Clonality Monoclonal
Clone SMI 34
ab24571 staining 200kDa + 160kDa Neurofilament (phospho) in mouse brain tissue sections by Immunohistochemistry (resin sections). Samples were incubated with primary antibody (1/500 in PBS + £% normal goat serum) for 20 hours at 4°C. A biotin-conjugated goat anti-mouse IgG polyclonal (1/400) was used as the secondary antibody.See Abreview

Anti-200kDa + 160kDa Neurofilament antibody [SMI-310] - ab24570 from Abcam

Host Mouse
Clonality Monoclonal
Clone SMI-310

Anti-Neurofilament (phospho) antibody - ab129349 from Abcam

Prices $407.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
ab129349, at 1/50 dilution, stains Neurofilament (phospho) in Formalin/PFA-fixed paraffin-embedded human brain cerebellum tissue by immunohistochemistry

Anti-Neurofilament antibody - ab93340 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Neurofilament antibody [LB-961] - ab128824 from Abcam

Host Mouse
Clonality Monoclonal
Clone LB-961

Anti-Neurofilament antibody [RNF406] - ab134306 from Abcam

Prices $378.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone RNF406
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cortex tissue labelling 140 kD + 160 kD Neurofilament with ab134306 at 10 µg/ml, using a biotinylated horse anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-NEFM / NF-M Antibody - LS-C195023 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µl
Host Chicken
Clonality Polyclonal
Immunofluorescent staining using NEFM antibody. Immunofluorescent staining of cultured rat neurons and glia showing labeling of NEFM in red
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