CHEK1

Gene Symbol CHEK1
Entrez Gene 1111
Alt Symbol CHK1
Species Human
Gene Type protein-coding
Description checkpoint kinase 1
Other Description CHK1 checkpoint homolog|Checkpoint, S. pombe, homolog of, 1|Chk1-S|cell cycle checkpoint kinase|checkpoint kinase-1|serine/threonine-protein kinase Chk1
Swissprots B5BTY6 B4DSK3 O14757 H2BI51 B4DDD0 A8K934 F5H7S4
Accessions AAM78553 AAQ76038 BAA84577 EAW67644 O14757 AA224205 AB451222 BAG70036 AB451345 BAG70159 AF016582 AAC51736 AF032874 AAB88852 AI750792 AK292549 BAF85238 AK293143 BAG56691 AK299783 BAG61665 AK300082 BAG61885 AW149126 BC004202 AAH04202 BC017575 AAH17575 BG191840 BG216281 BG218896 BG468734 BM455102 BP203333 BP207812 BX114781 BX419129 BX506561 CA441277 CA868104 DA567616 DQ892907 ABM83833 DQ896142 ABM87141 JF289264 AEB71796 XM_011542560 XP_011540862 XM_011542561 XP_011540863 XM_011542562 XP_011540864 XM_011542563 XP_011540865 NM_001114121 NP_001107593 NM_001114122 NP_001107594 NM_001244846 NP_001231775 NM_001274 NP_001265 NR_045204 NR_045205
Function Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser- 124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A. Inhibition of C
Subcellular Location Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Nuclear export is mediated at least in part by XPO1/CRM1. Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B.
Tissue Specificity Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon. {ECO:0000269|PubMed:9278511, ECO:0000269|PubMed:9382850}.
Top Pathways Cell cycle, p53 signaling pathway, HTLV-I infection, Viral carcinogenesis

PathScan ® Phospho-Chk1 (Ser317) Sandwich ELISA Kit - 7870 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1. Treatment of HeLa cells with UV stimulates phosphorylation of Chk1 at Ser317, detected by the PathScan® Phospho-Chk1 (Ser317) Sandwich ELISA Kit #7870, but does not affect the levels of total Chk1 detected by PathScan® Total Chk1 Sandwich ELISA Kit #7872. HeLa cells (80-90% confluent) were treated with 100 mJ/cm2 UV with 1 hour recovery at 37ºC. The absorbance readings at 450 nm are shown in the top figure, while the corresponding Western blots using Chk1 Antibody #2345 (left panel) or Phospho-Chk1 (Ser317) Antibody #2344 (right panel) are shown in the bottom figure.

PathScan ® Total Chk1 Sandwich ELISA Kit - 7872 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1. Treatment of HeLa cells with UV stimulates phosphorylation of Chk1 at Ser317, detected by the PathScan ® Phospho-Chk1 (Ser317) Sandwich ELISA Kit #7870, but does not affect the levels of total Chk1 detected by PathScan ® Total Chk1 Sandwich ELISA Kit #7872. HeLa cells (80-90% confluent) were treated with 100 mJ/cm2 UV with 1 hour recovery at 37ºC. The absorbance readings at 450 nm are shown in the top figure, while the corresponding Western blots using Chk1 Antibody #2345 (left panel) or Phospho-Chk1 (Ser317) Antibody #2344 (right panel) are shown in the bottom figure.

PathScan ® Total Chk1 Sandwich ELISA Antibody Pair - 7873 from Cell Signaling Technology

Category Antibody Pair
Prices $459.00
Sizes 1 kit (Reagents for 4 x 96 well plates)
The relationship between lysate protein concentration from untreated and UV treated HeLa cells and the absorbance at 450 nm using PathScan® Total Chk1 Sandwich ELISA Antibody Pair #7873 is shown. HeLa cells were treated with UV at 100 mj, incubated for 60 minutes at 37ºC and then lysed.

Human Phospho-CHEK1 / CHK1 (Ser296) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1248 from LifeSpan Bioscience

Category ELISA Kit
Prices $550.00
Sizes 1 plate

Human Phospho-CHEK1 / CHK1 (Ser286) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1337 from LifeSpan Bioscience

Category ELISA Kit
Prices $550.00
Sizes 1 plate

Mouse/Human/Rat Phospho-CHEK1 / CHK1 (Ser301) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1338 from LifeSpan Bioscience

Category ELISA Kit
Prices $550.00
Sizes 1 plate

Human Phospho-CHEK1 / CHK1 (Ser280) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1471 from LifeSpan Bioscience

Category ELISA Kit
Prices $550.00
Sizes 1 plate

Mouse/Human/Rat Phospho-CHEK1 / CHK1 (Ser317) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1472 from LifeSpan Bioscience

Category ELISA Kit
Prices $550.00
Sizes 1 plate

Mouse/Human/Rat CHEK1 / CHK1 ELISA Kit (Cell-Based ELISA) - LS-F1882 from LifeSpan Bioscience

Category ELISA Kit
Prices $450.00
Sizes 1 plate

Mouse CHEK1 / CHK1 ELISA Kit (Sandwich ELISA) - LS-F9408 from LifeSpan Bioscience

Category ELISA Kit
Prices $810.00
Sizes 1 plate
Sensitivity 0.058 ng/ml
Range 0.156 - 10 ng/ml

Rabbit anti-Chk1 Antibody, Affinity Purified - PLA0085 from Sigma-Aldrich

Prices $357.50
Sizes 100 µl
<B>Immunohistochemistry</B><BR/>Rabbit Anti-Chk1 Antibody, Affinity Purified: <B>Cat. No. PLA0085</B>: Detection of Human Chk1 by Immunohistochemistry. Sample: FFPE section of Human testicular seminoma. Antibody: Affinity purified Rabbit Anti-Chk1 (<B>Cat. No. PLA0085</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB staining.

Goat anti-Chk1 Antibody, Affinity Purified - PLA0054 from Sigma-Aldrich

Prices $357.50
Sizes 100 µl
<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Rabbit anti-Phospho Chk1 (S317) Antibody, Affinity Purified - PLA0056 from Sigma-Aldrich

Prices $386.00
Sizes 100 µl
<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Goat anti-Chk1 Antibody, Affinity Purified - PLA0055 from Sigma-Aldrich

Prices $357.50
Sizes 100 µl
<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Serine/threonine-protein kinase Chk1 (CHEK1), Human, ELISA Kit - E01S0334 from ARP American Research Products

Category ELISA Kit
Prices $535.00, $735.00
Sizes 48 t, 96 t

Chk1 Colorimetric Cell-Based ELISA Kit - CB5135 from Assay Biotechnology

Category ELISA Kit
Prices $380.00, $760.00, $1,140.00
Sizes 1 kit, 2 kits, 3 kits
Range > 5000 Cells

Chk1 (Phospho-Ser296) Colorimetric Cell-Based ELISA Kit - CBP1248 from Assay Biotechnology

Category ELISA Kit
Prices $480.00, $960.00, $1,440.00
Sizes 1 kit, 2 kits, 3 kits
Range > 5000 Cells

Chk1 (Phospho-Ser286) Colorimetric Cell-Based ELISA Kit - CBP1345 from Assay Biotechnology

Category ELISA Kit
Prices $480.00, $960.00, $1,440.00
Sizes 1 kit, 2 kits, 3 kits
Range > 5000 Cells

Chk1 (Phospho-Ser301) Colorimetric Cell-Based ELISA Kit - CBP1346 from Assay Biotechnology

Category ELISA Kit
Prices $480.00, $960.00, $1,440.00
Sizes 1 kit, 2 kits, 3 kits
Range > 5000 Cells

Chk1 (Phospho-Ser280) Colorimetric Cell-Based ELISA Kit - CBP1515 from Assay Biotechnology

Category ELISA Kit
Prices $480.00, $960.00, $1,440.00
Sizes 1 kit, 2 kits, 3 kits
Range > 5000 Cells

Chk1 (Phospho-Ser317) Colorimetric Cell-Based ELISA Kit - CBP1516 from Assay Biotechnology

Category ELISA Kit
Prices $480.00, $960.00, $1,440.00
Sizes 1 kit, 2 kits, 3 kits
Range > 5000 Cells

Checkpoint Homolog (CHEK1), ELISA Kit - MBS2000104 from MyBioSource

Category ELISA Kit
Prices $420.00, $625.00, $855.00, $3,510.00, $6,365.00
Sizes 24 -strip-wells, 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
Sensitivity Typically less than 0.058ng/mL.
Range 0.156-10ng/mL

Serine/threonine protein kinase Chk1 (CHEK1), ELISA Kit - MBS7242876 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Serine/threonine-protein kinase Chk1 (CHEK1), ELISA Kit - MBS7237645 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Serine/threonine-protein kinase Chk1 (CHEK1), ELISA Kit - MBS7202601 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
Search more