HRAS

Gene Symbol HRAS
Entrez Gene 3265
Alt Symbol C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
Species Human
Gene Type protein-coding
Description Harvey rat sarcoma viral oncogene homolog
Other Description GTP- and GDP-binding peptide B|GTPase HRas|Ha-Ras1 proto-oncoprotein|Harvey rat sarcoma viral oncoprotein|Ras family small GTP binding protein H-Ras|c-has/bas p21 protein|c-ras-Ki-2 activated oncogene|p19 H-RasIDX protein|transformation gene: oncogene HAMSV|transforming protein p21|v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Swissprots Q6FHV9 B5BUA0 Q9UCE2 Q14080 Q9BR65 P01112
Accessions AAA35683 AAA35685 AAA35687 AAA36557 AAA52693 AAB02605 AAK56963 ABI97389 CAA25624 CAA34461 CAN37622 CBV35641 CDL93477 EAX02337 EAX02338 EAX02339 EAX02340 EAX02341 P01112 AB451336 BAG70150 AB451485 BAG70299 AF493916 AAM12630 AJ437024 CAD24594 BC006499 AAH06499 BC095471 AAH95471 BM801632 BQ574535 BQ674260 BT019421 AAV38228 CR536579 CAG38816 CR542271 CAG47067 DN990077 KJ534867 AHW56507 KJ534868 AHW56508 XR_242795 XR_430702 NM_001130442 NP_001123914 NM_005343 NP_005334 NM_176795 NP_789765
Function Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. {ECO:0000269|PubMed:12740440, ECO:0000269|PubMed:14500341, ECO:0000269|PubMed:9020151}.
Subcellular Location Cell membrane. Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus. Golgi apparatus membrane; Lipid-anchor. Note=The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus. {ECO:0000250}.
Tissue Specificity Widely expressed. {ECO:0000269|PubMed:14500341}.
Top Pathways Central carbon metabolism in cancer, Axon guidance, Viral carcinogenesis, Estrogen signaling pathway, Sphingolipid signaling pathway

Ras (27H5) Rabbit mAb - 3339 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 27H5
Western blot analysis of extracts from 293T, C2C12 and C6 cells using Ras (27H5) Rabbit mAb.

Ras Antibody - 3965 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HT-29, PAE, NIH-3T3, AR42J, PC12 and C6 cell lysates,using Ras Antibody.

H-Ras (238) - sc-34 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rat
Clonality Monoclonal
Western blot analysis of human recombinant H-Ras fusion protein immunoprecipitated with H-Ras (238): sc-34 and detected with H-Ras (C-20): sc-520.

H-Ras (259) - sc-35 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rat
Clonality Monoclonal
H-Ras (259): sc-35. Western blot analysis of Ras p21 expression in KNRK whole cell lysate.

H-Ras (A-18) - sc-32025 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
H-Ras (A-18): sc-32025. Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing cytoplasmic staining of glandular cells.

H-Ras (C-20) - sc-520 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
H-Ras (C-20): sc-520. Western blot analysis of H-Ras expression in HeLa (A), Jurkat (B), KNRK (C) and NIH/3T3 (D) whole cell lysates.

H-Ras (E-15) - sc-68743 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
H-Ras (E-15): sc-68743. Western blot analysis of H-Ras expression in non-transfected 293T: sc-117752 (A), mouse H-Ras transfected 293T: sc-126931 (B) and HeLa (C) whole cell lysates.

H-Ras (F235) - sc-29 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
H-Ras (F235): sc-29. Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse colon tissue showing cytoplasmic localization.

H-Ras (M3) - sc-53958 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
H-Ras (M3): sc-53958. Western blot analysis of H-Ras expression in BJ (A) and human PBL (B) whole cell lysates.

H-Ras (M90) - sc-53959 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
H-Ras (M90): sc-53959. Western blot analysis of H-Ras expression in BJ (A) and human PBL (B) whole cell lysates.

H-Ras (R-12) - sc-32026 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-GTPase HRAS antibody - ab97488 from Abcam

Prices $385.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GTPase HRAS antibody (ab97488) at 1/1000 dilutionLane 1 : HeLa whole cell lysateLane 2 : HepG2 whole cell lysateLane 3 : MOLT4 whole cell lysateLysates/proteins at 30 µg/ml per lane.

Anti-GTPase HRAS antibody - ab96548 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab96548 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96548, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG(H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GTPase HRAS antibody - ab182776 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GTPase HRAS antibody (ab182776) at 1/400 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : 231 cell lysateLysates/proteins at 30 µg per lane.SecondaryDonkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution

Anti-GTPase HRAS antibody [1A1] - ab140962 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1A1
All lanes : Anti-GTPase HRAS antibody [1A1] (ab140962) at 1/200 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cells transfected with pCMV6-ENTRY GTPase HRAS cDNALysates/proteins at 5 µg per lane.

Anti-GTPase HRAS antibody [1C8] - ab139331 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1C8
All lanes : Anti-GTPase HRAS antibody [1C8] (ab139331) at 1/2000 dilutionLane 1 : Lysate of HEK293T cells transfected with pCMV6-ENTRY control Lane 2 : Lysate of HEK293T cells transfected with pCMV6-ENTRY GTPase HRASLysates/proteins at 5 µg per lane.

Anti-GTPase HRAS antibody [2A1] - ab140377 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2A1
All lanes : Anti-GTPase HRAS antibody [2A1] (ab140377) at 1/400 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cells transfected with pCMV6-ENTRY GTPase HRAS cDNALysates/proteins at 5 µg per lane.

Anti-GTPase HRAS antibody [H-Ras-03] - ab64705 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone H-Ras-03
All lanes : Anti-GTPase HRAS antibody [H-Ras-03] (ab64705) at 0.5 µg/mlLane 1 : HeLa human cervix carcinoma cell lineLane 2 : K567 human leukemia cell lineLane 3 : RAJI human Burkitt lymphoma cell linePerformed under reducing conditions.

Anti-HRAS antibody [1H3] - ab156784 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1H3
All lanes : Anti-HRAS antibody [1H3] (ab156784) at 1/200 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with pCMV6-ENTRY H-Ras cDNALysates/proteins at 5 µg per lane.

Anti-HRAS+KRAS antibody - ab53160 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HRAS+KRAS antibody (ab53160) at 1/500 dilutionLane 1 : HeLa cell extractLane 2 : HeLa cell extract with the immunising peptideLysates/proteins at 5 µg per lane.SecondaryLane 1 : Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L)Lane 2 : Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L)

Anti-N ras + c Ha ras antibody - ab24043 from Abcam

Prices $416.00
Sizes 50 µg
Host Sheep
Clonality Polyclonal

Anti-p21 Ras antibody [M337] - ab86696 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone M337
All lanes : Anti-p21 Ras antibody [M337] (ab86696) at 1/500 dilutionLane 1 : Mouse brainLane 2 : Human A431 cell lysateLane 3 : Jurkat cell lysate

Anti-Pan Ras antibody [C-4] - ab187096 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone C-4
All lanes : Anti-Pan Ras antibody [C-4] (ab187096) at 1/100 dilutionLane 1 : HISM whole cell lysateLane 2 : WI 38 whole cell lysateLane 3 : PC-3 whole cell lysateLane 4 : A-10 whole cell lysateLane 5 : KNRK whole cell lysate

Anti-Ras antibody - ab69747 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab69747 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69747, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Ras antibody - ab196671 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Ras antibody (ab196671) at 1/500 dilutionLane 1 : HeLa cell extractLane 2 : HeLa cell extract with immunizing peptide
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