AKT3

Gene Symbol AKT3
Entrez Gene 10000
Alt Symbol MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2
Species Human
Gene Type protein-coding
Description v-akt murine thymoma viral oncogene homolog 3
Other Description PKB gamma|RAC-gamma serine/threonine protein kinase|RAC-gamma serine/threonine-protein kinase|v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
Swissprots Q96QV3 Q5VTI1 Q5VTI2 Q9UFP5 Q9Y243 Q0VAA6
Accessions CAC22382 CAC93797 EAW77093 EAW77094 Q9Y243 AF085234 AAL40392 AF124141 AAD29089 AF135794 AAD24196 AJ245709 CAB53537 AK055109 AK308052 AL117525 CAB55977 AM392791 CAL37669 AM392849 CAL37727 AW960221 AY005799 AAF91073 BC019085 BC020479 AAH20479 BC121154 AAI21155 BG702262 U79271 XM_005272994 XP_005273051 XM_005272995 XP_005273052 XM_005272997 XP_005273054 XM_006711726 XP_006711789 XM_006724959 XP_006725022 XM_006724960 XP_006725023 XM_006724962 XP_006725025 XM_006724963 XP_006725026 XM_011544011 XP_011542313 XM_011544012 XP_011542314 XM_011544013 XP_011542315 XM_011544014 XP_011542316 XM_011546424 XP_011544726 XM_011546425 XP_011544727 XM_011546426 XP_011544728 XM_011546427 XP_011544729 NM_001206729 NP_001193658 NM_005465 NP_005456 NM_181690 NP_859029
Function AKT3 is one of 3 closely related serine/threonine- protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase- dependent apoptosis. {ECO:0000269|PubMed:18524868, EC
Subcellular Location Nucleus {ECO:0000269|PubMed:20018949}. Cytoplasm {ECO:0000269|PubMed:20018949}. Membrane {ECO:0000269|PubMed:20018949}; Peripheral membrane protein {ECO:0000269|PubMed:20018949}. Note=Membrane-associated after cell stimulation leading to its translocation.
Tissue Specificity In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
Top Pathways Apoptosis, Tight junction, VEGF signaling pathway, Focal adhesion, Toll-like receptor signaling pathway

PathScan ® Phospho-Akt3 (Ser472) Sandwich ELISA Kit (Mouse Preferred) - 7942 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)

PathScan ® Total Akt3 Sandwich ELISA Kit (Mouse Preferred) - 7934 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)

HTScan® Akt3 Kinase Assay Kit - 7507 from Cell Signaling Technology

Category Assay Kit
Figure 1. Akt3 kinase activity was measured in a radiometric assay using the following reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: GSK3 (14-27), 5 µg/50 µl, recombinant Akt3: 200 ng/50 µl.

Akt (pS473) ELISA Kit - ab126432 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.
Using the CREB1 assay kits CREB1 phosphorylation at Ser133 is detected in EGF-treated A431 cells (+), compared with untreated cells (-), whereas no change in total CREB1 levels was observed.

AKT 1/2/3 (pS473) PhosphoTracer ELISA Kit - ab119614 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
Sensitivity 10 pg/ml
Range 10 pg/ml - 10000 pg/ml
Using the PhosphoTracer AKT 1/2/3 (pS473) assay, the length of assay incubation time that was required to detect recombinant phospho-AKT was examined. After 15 mins, phospho-AKT concentrations of approximately 100 pg/mL were readily detected, while after 1 hour incubation, the limit of detection was less than 10 pg/mL. Longer incubation times had little effect on overall limit of detection that was observed.

AKT 1/2/3 (pS473) SimpleStep ELISA™ Kit - ab176635 from Abcam

Category ELISA Kit
Prices $484.00
Sizes 1 x 96 tests
Sensitivity 30 pg/ml
Range 1 ng/ml - 100 ng/ml

AKT1 Total PhosphoTracer ELISA Kit - ab119616 from Abcam

Category ELISA Kit
Sensitivity 100 pg/ml
Range 100 pg/ml - 100000 pg/ml
Detection of total AKT1 in MCF7 cells treated with various concentration of wortmannin, using ab119616.

AKT1 Total SimpleStep ELISA™ Kit - ab176637 from Abcam

Category ELISA Kit
Prices $484.00
Sizes 1 x 96 tests
Sensitivity 60 pg/ml
Range 0.5 ng/ml - 50 ng/ml

ERK 1/2 (pT202/Y204) PhosphoTracer ELISA Kit - ab119625 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
Sensitivity 10 pg/ml
Range 10 pg/ml - 10000 pg/ml
As shown, using the ERK 1/2 assay kits, a significant reduction of ERK 1/2 phosphorylation at Thr202/Tyr204 is detected in A431 cells treated with AG1478 for 3 hours compared with untreated cells, while only a small change in total ERK levels is observed.

ERK 1/2 Total PhosphoTracer ELISA Kit - ab119626 from Abcam

Category ELISA Kit
Sensitivity 100 pg/ml
Range 100 pg/ml - 100000 pg/ml
As shown, using the ERK 1/2 assay kits, a significant reduction of ERK 1/2 phosphorylation at Thr202/Tyr204 is detected in A431 cells treated with AG1478 for 3 hours compared with untreated cells, while only a small change in total ERK levels is observed.

ERK1/2 (pT202/Y204) SimpleStep ELISA™ Kit - ab176640 from Abcam

Category ELISA Kit
Prices $484.00
Sizes 1 x 96 tests
Sensitivity < 0.1 ng/ml
Range 0.2 ng/ml - 20 ng/ml
Example of a typical ERK1/2 (pT202/Y204) cell lysate dilution series. Background-subtracted data values (mean +/- SD) are graphed.

ERK1/2 Total SimpleStep ELISA™ Kit - ab176641 from Abcam

Category ELISA Kit
Prices $484.00
Sizes 1 x 96 tests
Sensitivity < 0.6 ng/ml
Range 0.6 ng/ml - 60 ng/ml
Example of a typical ERK1/2 cell lysate dilution series. Background-subtracted data values (mean +/- SD) are graphed.

Human AKT3 ELISA Kit (Sandwich ELISA) - LS-F4162 from LifeSpan Bioscience

Category ELISA Kit
Prices $650.00
Sizes 1 plate
Sensitivity 1.56 ng/ml
Range 1.56-100 ng/ml

Human PKBg ELISA Kit - abx152751 from Abbexa

Category ELISA Kit
Sensitivity 0.56 ng/ml
Range 1.563 ng/ml - 100 ng/ml

Protein Kinase B Gamma, PKBG, Human, ELISA Kit - E-EL-H1175 from ARP American Research Products

Category ELISA Kit
Prices $522.00
Sizes 96 t

Protein Kinase B Gamma (PKBg), Human, ELISA Kit - SEA382Hu from ARP American Research Products

Category ELISA Kit
Prices $650.00
Sizes 96 t
Sensitivity 0.56ng/mL
Range 1.56-100ng/mL

Protein Kinase B Gamma, PKBg, Mouse, ELISA Kit - E-EL-M0971 from ARP American Research Products

Category ELISA Kit
Prices $585.00
Sizes 96 t

Protein Kinase B Gamma, PKBG, Rat, ELISA Kit - E-EL-R0806 from ARP American Research Products

Category ELISA Kit
Prices $585.00
Sizes 96 t

Protein Kinase B Gamma, PKBG, Rabbit, ELISA Kit - E-EL-RB0824 from ARP American Research Products

Category ELISA Kit
Prices $585.00
Sizes 96 t

Protein Kinase B Gamma, PKBG, Chicken, ELISA Kit - E-EL-Ch0292 from ARP American Research Products

Category ELISA Kit
Prices $445.50
Sizes 96 t

Protein Kinase B Gamma, PKBG, Porcine, ELISA Kit - E-EL-P0424 from ARP American Research Products

Category ELISA Kit
Prices $585.00
Sizes 96 t

Protein Kinase B Gamma, PKBG, Sheep, ELISA Kit - E-EL-S0287 from ARP American Research Products

Category ELISA Kit
Prices $648.00
Sizes 96 t

Protein Kinase B Gamma, PKBG, Guinea Pig, ELISA Kit - E-EL-GP0952 from ARP American Research Products

Category ELISA Kit
Prices $522.00
Sizes 96 t

Protein Kinase B Gamma, PKBG, Monkey, ELISA Kit - E-EL-MK1948 from ARP American Research Products

Category ELISA Kit
Prices $585.00
Sizes 96 t
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