ACTA2

Gene Symbol ACTA2
Entrez Gene 59
Alt Symbol AAT6, ACTSA, MYMY5
Species Human
Gene Type protein-coding
Description actin, alpha 2, smooth muscle, aorta
Other Description actin, aortic smooth muscle|alpha-cardiac actin|cell growth-inhibiting gene 46 protein
Swissprots B2R8A4 P62736 Q6FI19
Accessions AAA51586 AAA60560 ACZ58370 BAA00492 EAW50153 EAW50154 EAW50155 P62736 AK127229 AK300353 BAH13267 AK300664 BAG62350 AK313294 BAG36101 AY692464 AAW29811 BC017554 AAH17554 BC093052 AAH93052 BP379410 BQ029841 CR536518 CAG38756 DC304647 DC354672 DC385559 DQ892391 ABM83317 DQ895606 ABM86532 J05192 AAA51577 X13839 CAA32064 XM_011540016 XP_011538318 NM_001141945 NP_001135417 NM_001613 NP_001604
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subcellular Location Cytoplasm, cytoskeleton.
Top Pathways Vascular smooth muscle contraction

α-Actin (1A4) - sc-32251 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
α-Actin (1A4): sc-32251. Western blot analysis of α-Actin expression in IMR-32 (A) and C2C12 (B) whole cell lysates.

α-Actin (a-SM1) - sc-130616 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
α-Actin (a-SM1): sc-130616. Western blot analysis of ACTA2 expression in non-transfected: sc-110760 (A) and human ACTA2 transfected: sc-111833 (B) 293 whole cell lysates and mouse heart tissue extract (C).

α-Actin (alpha-SM1) - sc-130617 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
α-Actin (alpha-SM1): sc-130617. Western blot analysis of ACTA2 expression in non-transfected: sc-110760 (A) and human ACTA2 transfected: sc-111833 (B) 293 whole cell lysates and mouse heart tissue extract (C).

α-Actin (5C5) - sc-58670 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
α-Actin (5C5): sc-58670. Western blot analysis of α-Actin expression in mouse skeletal muscle (A) and mouse heart (B) tissue extracts.

Anti-alpha smooth muscle Actin antibody - ab21027 from Abcam

Prices $391.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-alpha smooth muscle Actin antibody (ab21027) at 0.3 µg/ml + human duodenum lysate at 35 µgdeveloped using the ECL technique

Anti-alpha smooth muscle Actin antibody - ab54723 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-alpha smooth muscle Actin antibody (ab54723) at 1 µg/ml + Whole tissue lysate prepared from human omentum, serous carcinoma.

Anti-alpha smooth muscle Actin antibody - ab112022 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Immunohistochemical expression of alpha smooth muscle Actin in Formalin-Fixed, Paraffin-Embedded Human Colon, smooth muscle using ab112022 at 2.5 µg/ml.

Anti-alpha smooth muscle Actin antibody [1A4] (Biotin) - ab125057 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1A4
IHC image of ab125057 staining in human breast ductal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab125057, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-alpha smooth muscle Actin antibody [4A4] - ab119952 from Abcam

Prices $387.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4A4
All lanes : Anti-alpha smooth muscle Actin antibody [4A4] (ab119952) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : A431 cell lysateLane 3 : Jurkat cell lysateLane 4 : K562 cell lysateLane 5 : HEK293 cell lysateLane 6 : HepG2 cell lysateLane 7 : NIH3T3 cell lysateLane 8 : PC12 cell lysateLane 9 : COS7 cell lysate

Anti-alpha smooth muscle Actin antibody [alpha sm-1] - ab49481 from Abcam

Prices $359.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone alpha sm-1
Ab49481 staining human myometrium tissue. Staining is localised to cytoplasm.Left panel: with primary antibody diluted 1:1000. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-alpha smooth muscle Actin antibody [E184] - ab32575 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E184
Anti-alpha smooth muscle Actin antibody [E184] (ab32575) at 1/50000 dilution + A431 cell lysate

Anti-alpha smooth muscle Actin antibody [E184] (Alexa Fluor® 488) - ab197240 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E184
Overlay histogram showing HeLa cells stained with ab197240 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab197240, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in HeLa fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-alpha smooth muscle Actin antibody [E184] (Alexa Fluor® 647) - ab196919 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E184
ab196919 staining alpha Smooth Muscle Actin in HeLa cells. The cells were fixed with 4% PFA (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196919 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-alpha smooth muscle Actin antibody [E184] (HRP) - ab196920 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E184
Anti-alpha smooth muscle Actin antibody [E184] (HRP) (ab196920) at 1/5000 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgdeveloped using the ECL techniquePerformed under reducing conditions.

Anti-alpha smooth muscle Actin antibody [EPR5368] - ab124964 from Abcam

Prices $398.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5368
All lanes : Anti-alpha smooth muscle Actin antibody [EPR5368] (ab124964) at 1/10000 dilution (purified)Lane 1 : HeLa cell lysateLane 2 : HEK293 cell lysateLane 3 : U937 cell lysateLane 4 : A431 cell lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Anti-alpha smooth muscle Actin antibody [EPR5368] (Alexa Fluor® 555) - ab202509 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5368
ab202509 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202509 at 1/200 dilution (shown in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-alpha smooth muscle Actin antibody [EPR5368] (Alexa Fluor® 568) - ab201847 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5368
ab201847 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201847 at 1/200 dilution (shown in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-smooth muscle Actin antibody - ab125044 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab125044, at 1/100, staining smooth muscle Actin in formalin fixed, paraffin embedded Human small intestine tissue by Immunohistochemistry.

Anti-smooth muscle Actin antibody [1A4] - ab134813 from Abcam

Prices $395.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 1A4
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate, smooth muscle tissue labelling smooth muscle Actin with ab134813 at 1/50 dilution.

Anti-ACTA2 / Smooth Muscle Actin Antibody (clone a-SM1) - LS-C26843 from LifeSpan Bioscience

Prices $410.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone a-SM1

Anti-ACTA2 / Smooth Muscle Actin Antibody (N-Terminus, TAMRA) - LS-C33054 from LifeSpan Bioscience

Prices $315.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-ACTA2 / Smooth Muscle Actin Antibody (N-Terminus, HiLyte Fluor 555) - LS-C33058 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-ACTA2 / Smooth Muscle Actin Antibody (N-Terminus, HiLyte Fluor) - LS-C33057 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-ACTA2 / Smooth Muscle Actin Antibody (N-Terminus, AMCA) - LS-C33055 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-ACTA2 / Smooth Muscle Actin Antibody (N-Terminus, HiLyte Fluor 488) - LS-C33060 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
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