BUB1

Gene Symbol BUB1
Entrez Gene 699
Alt Symbol BUB1A, BUB1L, hBUB1
Species Human
Gene Type protein-coding
Description BUB1 mitotic checkpoint serine/threonine kinase
Other Description BUB1 budding uninhibited by benzimidazoles 1 homolog|budding uninhibited by benzimidazoles 1 homolog|mitotic checkpoint serine/threonine-protein kinase BUB1|mitotic spindle checkpoint kinase|putative serine/threonine-protein kinase
Swissprots F5GXI5 O43643 E9PC26 O43683 Q53QE4 O60626 O43430
Accessions AAD43675 AAK29549 AAY14706 CBJ05771 CBJ21223 EAW50355 O43683 AF011387 AAC39546 AF043294 AAB97855 AF046078 AAC12729 AF047471 AAC03122 AF053305 AAC06259 AI635394 AK023540 AK225317 AK291221 BAF83910 AK302418 BAG63724 BC017038 AAH17038 BC028201 AAH28201 BP313176 DB453210 JF432720 ADZ15937 XR_923001 NM_001278616 NP_001265545 NM_001278617 NP_001265546 NM_004336 NP_004327
Function Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activat
Subcellular Location Nucleus. Chromosome, centromere, kinetochore. Note=Nuclear in interphase cells. Accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. Localizes to the outer kinetochore. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage and occurs very early in prophase. AURKB, CASC5 and INCENP are required for kinetochore localization (By similarity). {ECO:0000250}.
Tissue Specificity High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index.
Top Pathways Cell cycle, Progesterone-mediated oocyte maturation, Oocyte meiosis

BUB1 (B-3) - sc-365685 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
BUB1 (B-3): sc-365685. Western blot analysis of BUB1 expression in HeLa (A) and K-562 (B) whole cell lysates.

BUB1 (H-300) - sc-28257 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

BUB1 (E-20) - sc-31101 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

BUB1 (14H5) - sc-47743 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
BUB1 (14H5): sc-47743. Western blot analysis of BUB1 expression in K-562 whole cell lysate.

BUB1 (N-20) - sc-18286 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
BUB1 (N-20): sc-18286. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.

BUB1 (Y-18) - sc-31100 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Bub1 antibody - ab9000 from Abcam

Prices $382.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Bub1 antibody - ab54893 from Abcam

Prices $390.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
ab54893 (1/400) staining Bub1 in Assynchronous Hela cells (green). Cells were fixed in methanol and counterstained with DAPI (red). Please refer to Abreview for further experimental details.See Abreview

Anti-Bub1 antibody - ab70372 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling Bub1 with ab70372 at 1/1000 (1µg/ml). Detection: DAB.

Anti-Bub1 antibody - ab79059 from Abcam

Prices $378.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of Bub1 expression in paraffin embedded formalin fixed human small intestine using 5 µg/ml ab79059

Anti-Bub1 antibody - ab87889 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Bub1 antibody (ab87889) at 1 µg/ml + HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Bub1 antibody [14H5] - ab181438 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 14H5

Anti-Bub1 antibody [2096C1a] - ab51269 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2096C1a
Anti-Bub1 antibody [2096C1a] (ab51269)

Anti-Bub1 antibody [EPR12249(2)] - N-terminal - ab183026 from Abcam

Host Rabbit
Clonality Monoclonal
Clone EPR12249(2)
Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling Bub1 with ab183026 at 1/80 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Anti-BUB1 Antibody (aa281-419) - LS-C18935 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-BUB1 Antibody (aa281-419) - LS-C45011 from LifeSpan Bioscience

Prices $405.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-BUB1 Antibody - LS-C77243 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-BUB1 Antibody - LS-C77244 from LifeSpan Bioscience

Prices $530.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-BUB1 Antibody (clone 14H5) - LS-C82213 from LifeSpan Bioscience

Prices $450.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 14H5
Ovarian cancer cells were arrested with Nocodazole before fixation; primary Ab: anti-Bub1 mAb (clone 14H5); Secondary Ab: anti-mouse Alexa 488; chromosome visualization: DAPI

Anti-BUB1 Antibody (clone P8.14H5.1H1) - LS-C137473 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone P8.14H5.1H1

Anti-BUB1 Antibody (aa251-300) - LS-C118685 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human breast carcinoma tissue, using BUB1 Antibody. The picture on the right is treated with the synthesized peptide.

Anti-BUB1 Antibody (clone 2096C1a) - LS-C184353 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2096C1a

Anti-BUB1 Antibody (Phe818) - LS-C193180 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BUB1 Antibody (C-Terminus, AP) - LS-C275677 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BUB1 Antibody (C-Terminus, APC) - LS-C275678 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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