ATP1A1

Gene Symbol ATP1A1
Entrez Gene 476
Alt Symbol -
Species Human
Gene Type protein-coding
Description ATPase, Na+/K+ transporting, alpha 1 polypeptide
Other Description Na(+)/K(+) ATPase alpha-1 subunit|Na+, K+ ATPase alpha subunit|Na+/K+ ATPase 1|Na, K-ATPase, alpha-A catalytic polypeptide|Na,K-ATPase alpha-1 subunit|Na,K-ATPase catalytic subunit alpha-A protein|sodium pump 1|sodium pump subunit alpha-1|sodium-potassium ATPase catalytic subunit alpha-1|sodium-potassium-ATPase, alpha 1 polypeptide|sodium/potassium-transporting ATPase subunit alpha-1
Swissprots Q6LDM4 Q16689 Q9UJ21 B2RBR6 B7Z2T5 P05023 Q9UCN1 F5H3A1 B7Z3U6 Q9UJ20
Accessions AAA35573 AAA35574 AAA51801 AAA92713 EAW56644 EAW56645 EAW56646 EAW56647 EAW56648 P05023 AK293689 BAH11571 AK295095 BAH11971 AK296362 BAH12332 AK296384 BAH12337 AK308418 AK314777 BAG37313 AL531892 AY642124 AY946013 AAX55908 BC001330 AAH01330 BC003077 AAH03077 BC050359 AAH50359 BF795089 DC335233 DQ891882 ABM82808 EU176649 ABW03450 J03007 AAA51803 M16793 AAD56251 M16794 AAD56252 U16798 AAC50131 XM_006710655 XP_006710718 NM_000701 NP_000692 NM_001160233 NP_001153705 NM_001160234 NP_001153706 NM_001001586
Function This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Subcellular Location Cell membrane; Multi-pass membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Top Pathways Salivary secretion, Cardiac muscle contraction, cAMP signaling pathway, Aldosterone-regulated sodium reabsorption, Thyroid hormone synthesis

Phospho-Na,K-ATPase α1 (Tyr10) (E1Y9C) Rabbit mAb - 13566 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone E1Y9C
Western blot analysis of extracts from A-431 cells, untreated (-) or treated with Human Epidermal Growth Factor (hEGF) #8916 (100 ng/ml, 2 min; +), and HeLa cells, untreated (-) or pervanadate-treated (1 mM, 15 min; +), using Phospho-Na,K-ATPase α1 (Tyr10) (E1Y9C) Rabbit mAb (upper) and Na,K-ATPase Antibody #3010 (lower).

Phospho-Na,K-ATPase α1 (Ser16) Antibody - 4020 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from PC-12 cells, untreated or TPA-treated, using Phospho-Na,K-ATPase α1 (Ser16) Antibody (upper) or total Na/K ATPase α1 Antibody #3010 (lower).

Phospho-Na,K-ATPase α1 (Tyr10) Antibody - 3060 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells, untreated or vanadate-treated, and A431 cells, untreated or EGF-treated, using Phospho-Na,K-ATPase α1 (Tyr10) Antibody (upper) or total Na,K-ATPase α1 Antibody #3010 (lower).

Na,K-ATPase Antibody - 3010 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using Na,K-ATPase α Antibody.

Na + /K + -ATPase α (H-3) - sc-48345 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Na<sup>+</sup>/K<sup>+</sup>-ATPase α (H-3): sc-48345. Western blot analysis of Na<sup>+</sup>/K<sup>+</sup>-ATPase α expression in MDCK (A) and HeLa (B) whole cell lysates.

Na + /K + -ATPase α (H-300) - sc-28800 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Na<sup>+</sup>/K<sup>+</sup>-ATPase α (H-300): sc-28800. Western blot analysis of Na<sup>+</sup>/K<sup>+</sup>-ATPase α expression in HeLa (A) and Caki-1 (B) whole cell lysates.

Na + /K + -ATPase α (M7-PB-E9) - sc-58628 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Na<sup>+</sup>/K<sup>+</sup>-ATPase α (M7-PB-E9): sc-58628. Western blot analysis of Na<sup>+</sup>/K<sup>+</sup>-ATPase α expression in MDCK (A) and HeLa (B) whole cell lysates.

p-Na + /K + -ATPase α (Ser 943) - sc-16710 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Na + /K + -ATPase α1 (9-A5) - sc-58629 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Na<sup>+</sup>/K<sup>+</sup>-ATPase α1 (9-A5): sc-58629. Western blot analysis of Na<sup>+</sup>/K<sup>+</sup>-ATPase α1 expression in HeLa (A) and MDCK (B) whole cell lysates.

Na + /K + -ATPase α1 (C-20) - sc-16043 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Na<sup>+,/sup>/K<sup>+</sup>-ATPase α1 (C-20): sc-16043. Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tumor showing membrane localization.

Na + /K + -ATPase α1 (M8-P1-A3) - sc-58630 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Na + /K + -ATPase α1 (N-15) - sc-16041 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Na<sup>+</sup>/K<sup>+</sup>-ATPase α1 (N-15): sc-16041. Western blot analysis of Na<sup>+</sup>/K<sup>+</sup>-ATPase α1 expression in PMA-treated KNRK whole cell lysate.

Na + /K + -ATPase α1 (6D133) - sc-71637 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Na + /K + -ATPase α1 (6D134) - sc-71639 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

p-Na + /K + -ATPase α1 (Ser 16) - sc-109465 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-alpha 1 Sodium Potassium ATPase (phospho S16) antibody - ab195932 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-alpha 1 Sodium Potassium ATPase (phospho S16) antibody (ab195932) at 1/500 dilutionLane 1 : PMA treated 293 whole cell lysate with antigen-specific peptideLane 2 : PMA treated 293 whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-alpha 1 Sodium Potassium ATPase (phospho S16) antibody - ab194532 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-alpha 1 Sodium Potassium ATPase (phospho S16) antibody (ab194532) at 1/500 dilutionLane 1 : 293 cell extract, treated with PMALane 2 : 293 cell extract, treated with PMA with antigen-specific peptide

Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)] - ab124677 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2375(2)
All lanes : Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)] (ab124677) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : HeLa cell lysate, treated with PervanadateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-alpha 1 Sodium Potassium ATPase antibody - ab133211 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of COS7 cells labelling alpha 1 Sodium Potassium ATPase with ab133211 at 1/100 dilution (left). Right panel is treated with the immunizing peptide.

Anti-alpha 1 Sodium Potassium ATPase antibody - ab196884 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of HeLa cells, labeling alpha 1 Sodium Potassium ATPase using ab196884 at a 1/100 dilution. Right image treated with synthesized peptide.

Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free - ab174786 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 464.6

Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker - ab7671 from Abcam

Prices $400.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 464.6
Lanes 1 - 3 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671) at 1 µg/mlLanes 4 - 6 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671) at 2 µg/mlLanes 7 - 9 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671) at 5 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Mouse) Tissue Lysate (ab27253)Lane 3 : Brain (Rat) Tissue Lysate (ab7942)Lane 4 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 5 : Brain (Mouse) Tissue Lysate (ab27253)Lane 6 : Brain (Rat) Tissue Lysate (ab7942)Lane 7 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 8 : Brain (Mouse) Tissue Lysate (ab27253)Lane 9 : Brain (Rat) Tissue Lysate (ab7942)Lysates/proteins at 20 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (Alexa Fluor® 488) - ab197496 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 464.6
ab197496 staining alpha 1 Sodium Potassium ATPase in Hek293 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197496 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in Hek293 cells fixed with 4% formaldehyde (10 min).

Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (Alexa Fluor® 647) - ab196695 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 464.6
ab196695 staining alpha 1 Sodium Potassium ATPase in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab196695 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution, overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (HRP) - ab196696 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 464.6
IHC image of alpha 1 Sodium Potassium ATPase staining in a section of formalin-fixed paraffin-embedded normal human kidney*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196696 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Search more