APEX1

Gene Symbol	APEX1
Entrez Gene	328
Alt Symbol	APE, APE1, APEN, APEX, APX, HAP1, REF1
Species	Human
Gene Type	protein-coding
Description	APEX nuclease (multifunctional DNA repair enzyme) 1
Other Description	AP endonuclease class I|AP lyase|DNA-(apurinic or apyrimidinic site) lyase|apurinic-apyrimidinic endonuclease 1|apurinic/apyrimidinic (abasic) endonuclease|deoxyribonuclease (apurinic or apyrimidinic)|protein REF-1|redox factor-1
Swissprots	Q99775 Q969L5 P27695
Accessions	AAA58373 AAA58629 AAL86909 BAA02633 CAA46925 EAW66463 EAW66464 EAW66465 EAW66466 EAW66467 EAW66468 P27695 AI554786 AK098588 AK291100 BAF83789 AK311548 BC002338 AAH02338 BC004979 AAH04979 BC008145 AAH08145 BC019291 AAH19291 BC095428 AAH95428 BT007236 AAP35900 BT020133 AAV38935 BX161454 CAD61917 CB139337 D90373 BAA14381 DQ891814 ABM82740 DQ894998 ABM85924 M80261 AAA58371 M81955 AAA58372 S43127 AAB22977 U79268 AAB50212 X59764 CAA42437 XM_005267581 XP_005267638 XM_005267582 XP_005267639 NM_001244249 NP_001231178 NM_001641 NP_001632 NM_080648 NP_542379 NM_080649 NP_542380
Function	Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3'
Subcellular Location	Nucleus. Nucleus, nucleolus. Nucleus speckle. Endoplasmic reticulum. Cytoplasm. Note=Detected in the cytoplasm of B-cells stimulated to switch (By similarity). Colocalized with SIRT1 in the nucleus. Colocalized with YBX1 in nuclear speckles after genotoxic stress. Together with OGG1 is recruited to nuclear speckles in UVA-irradiated cells. Colocalized with nucleolin and NPM1 in the nucleolus. Its nucleolar localization is cell cycle dependent and requires active rRNA transcription. Colocalized with calreticulin in the endoplasmic reticulum. Translocation from the nucleus to the cytoplasm is stimulated in presence of nitric oxide (NO) and function in a CRM1-dependent manner, possibly as a consequence of demasking a nuclear export signal (amino acid position 64-80). S-nitrosylation at Cys-93 and Cys-310 regulates its nuclear-cytosolic shuttling. Ubiquitinated form is localized predominantly in the cytoplasm. {ECO:0000250}.
Top Pathways	Base excision repair

• Antibodies
• Assays/Kits
• Proteins/Peptides
• siRNAs/Functional Genomics
• cDNA Clones
• Cell/Tissue/Blood Products

Mouse/Human/Rat APEX1 / APE1 ELISA Kit (Cell-Based ELISA) - LS-F3079 from LifeSpan Bioscience Category ELISA Kit Prices $450.00 Sizes 1 plate Range 0.156-10 ng/ml

Mouse APEX1 / APE1 ELISA Kit (Sandwich ELISA) - LS-F9436 from LifeSpan Bioscience Category ELISA Kit Prices $740.00 Sizes 1 plate Sensitivity 0.156 ng/ml Range 0.156-10 ng/ml

Human APEX1 / APE1 ELISA Kit (Sandwich ELISA) - LS-F10852 from LifeSpan Bioscience Category ELISA Kit Prices $600.00 Sizes 1 plate Sensitivity 0.312 Range 0.156-10 ng/ml

Human Total APE DuoSet IC ELISA - DYC1044-2 from R&D Systems Category ELISA Kit Prices $329.00 Sizes 1 kit (for 2 Plates) Range 469.00 - 30,000 pg/mL

DNA-(apurinic or apyrimidinic site) lyase, APEX1, Human, ELISA Kit - E1939h from ARP American Research Products Category ELISA Kit Prices $596.00 Sizes 96 t Range 0.312-20 ng/mL

APEX1 (Human) IP-WB Antibody Pair - H00000328-PW1 from Acris Antibodies Category Antibody Pair Prices $610.00 Sizes 1 set

APEX1 (Human) IP-WB Antibody Pair - H00000328-PW2 from Acris Antibodies Category Antibody Pair Prices $610.00 Sizes 1 set

APEX1 Colorimetric Cell-Based ELISA - CB5867 from Assay Biotechnology Category ELISA Kit Prices $380.00, $760.00, $1,140.00 Sizes 1 kit, 2 kits, 3 kits Range > 5000 Cells

APEX nuclease (multifunctional DNA repair enzyme) 1, ELISA Kit - MBS941991 from MyBioSource Category ELISA Kit Prices $250.00, $495.00, $710.00, $2,705.00, $5,145.00 Sizes 24 -strip-wells (Limit 1), 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells Sensitivity 3.9 pg/ml. Range 15.6 pg/ml -1000 pg/ml.

ELISA Kit for Apurinic/Apyrimidinic Endonuclease 1 (APEX1) - SEK040Mu from Uscn Life Science Inc. Category ELISA Kit Sensitivity The minimum detectable dose of this kit is typically less than 0.051ng/mL. Range 0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL

Human DNA-(apurinic or apyrimidinic site) lyase (APEX1) ELISA kit - CSB-EL001900HU from Cusabio Category ELISA Kit Sensitivity 3.9 pg/ml. Range 15.6 pg/ml-1000 pg/ml

Bovine APEX1 / APE1 ELISA Kit (Custom ELISA) - LS-F18862 from LifeSpan Bioscience Category ELISA Kit Prices $700.00 Sizes 1 plate Sensitivity To be determined Range 0.156-10 ng/ml

Rat APEX1 / APE1 ELISA Kit (Sandwich ELISA) - LS-F18863 from LifeSpan Bioscience Category ELISA Kit Prices $600.00 Sizes 1 plate Sensitivity 0.156 Range 0.156-10 ng/ml

Mouse APEX1 / APE1 ELISA Kit (Custom ELISA) - LS-F18864 from LifeSpan Bioscience Category ELISA Kit Prices $600.00 Sizes 1 plate Sensitivity To be determined Range 0.156-10 ng/ml

APEX1 ELISA Kit - DEIA-XYA223 from Creative Diagnostics Category ELISA Kit

APEX1 - EKH4018 from Nordic BioSite Sensitivity 0.188ng/ml Range 0.312-20ng/ml

APEX1 ELISA Kit (Human) : 96 Wells (OKEH01380) - OKEH01380 from Aviva Systems Biology Category ELISA Kit Prices $420.00 Sizes 96 wells Sensitivity < 0.078 ng/mL Range 0.312 - 20 ng/mL

Custom Human APE1 ELISA - ELH-APE1-1 from RayBiotech Category ELISA Kit Prices $627.00, $1,128.60, $2,508.00 Sizes 1 plate kit, 2 plate kit, 5 plate kit

Custom Human APE1 ELISA Kit - orb384469 from Biorbyt Category ELISA Kit Prices $1,411.00, $2,448.00, $5,321.00 Sizes 96 assays, 192 assays, 480 assays

Human APEX1 ELISA Kit - E003231 from Biobool Category ELISA Kit Sensitivity 3.9 pg/ml.

Human APX ELISA Kit - E003792 from Biobool Category ELISA Kit

Human DNA-(apurinic or apyrimidinic site) lyase ELISA Kit - EK4108 from Signalway Antibody Category ELISA Kit Prices $599.00 Sizes 1x96t Range 0.156-10 ng/mL

anti-APE1 antibody - ARG55293 from Arigo Biolaboratories Category ELISA Kit

anti-APE1 antibody - ARG63219 from Arigo Biolaboratories Category ELISA Kit

DNA-(apurinic or apyrimidinic site) Lyase (APEX1), ELISA Kit - MBS9376516 from MyBioSource Category ELISA Kit Prices $435.00, $600.00, $2,650.00, $5,205.00 Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

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