ATP5O

Gene Symbol ATP5O
Entrez Gene 539
Alt Symbol ATPO, OSCP
Species Human
Gene Type protein-coding
Description ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit
Other Description ATP synthase subunit O, mitochondrial|human ATP synthase OSCP subunit|oligomycin sensitivity conferral protein|oligomycin sensitivity conferring protein
Swissprots P48047 B2R4E2 Q6IBI2 Q5U042
Accessions EAX09802 EAX09803 EAX09804 P48047 AF088071 AK093795 AK124187 AK222608 BAD96328 AK222962 BAD96682 AK311796 BAG34739 AV734214 AW518473 BC021233 AAH21233 BC022865 AAH22865 BT019836 AAV38639 CD174837 CR456822 CAG33103 DQ656021 DQ656022 JF432864 ADZ16081 S77356 AAB34321 X83218 CAA58219 X83219 NM_001697 NP_001688
Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
Subcellular Location Mitochondrion {ECO:0000250}. Mitochondrion inner membrane {ECO:0000250}.
Top Pathways Alzheimer's disease, Huntington's disease, Oxidative phosphorylation

OSCP (A-8) - sc-365162 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
OSCP (A-8): sc-365162. Western blot analysis of OSCP expression in HeLa (A) and HEK293 (B) whole cell lysates and mouse heart tissue extract (C).

OSCP (H-140) - sc-98707 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
OSCP (H-140): sc-98707. Western blot analysis of OSCP expression in mouse heart (A) and human heart (B) tissue extracts.

OSCP (Y-17) - sc-74789 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

OSCP (C-16) - sc-74786 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
OSCP (C-16): sc-74786. Western blot analysis of OSCP expression in non-transfected: sc-117752 (A) and mouse OSCP transfected: sc-122274 (B) 293T whole cell lysates and mouse heart tissue extract (C).

Anti-ATP5O antibody - ab91400 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-ATP5O antibody (ab91400) at 1/100 dilution + 293 cell lysate at 35 µg

Anti-ATP5O antibody - ab154232 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-ATP5O antibody (ab154232) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

Anti-ATP5O antibody [4C11C10D12] - ab110276 from Abcam

Prices $391.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4C11C10D12
All lanes : Anti-ATP5O antibody [4C11C10D12] (ab110276) at 0.5 µg/mlLane 1 : Human heart mitochondria at 5 µgLane 2 : Bovine heart mitochondria at 1 µg

Anti-ATP5O antibody [4C11C10D12] (Alexa Fluor® 488) - ab198302 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4C11C10D12
ab198302 staining ATP5O in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198302 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLacells fixed with 4% formaldehyde (10 min).

Anti-ATP5O antibody [4C11C10D12] (Alexa Fluor® 647) - ab198103 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4C11C10D12
ab198103 staining ATP50 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab198103 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-ATP5O Antibody (aa44-73) IHC-plus™ - LS-B9288 from LifeSpan Bioscience

Prices $395.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-ATP5O antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-ATP5O Antibody (N-Terminus, AP) - LS-C275288 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ATP5O Antibody (N-Terminus, APC) - LS-C275289 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ATP5O Antibody (N-Terminus, Biotin) - LS-C275290 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ATP5O Antibody (N-Terminus, FITC) - LS-C275291 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ATP5O Antibody (N-Terminus, Azide-free, HRP) - LS-C275292 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ATP5O Antibody (N-Terminus, PE) - LS-C275294 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ATP5O Antibody (N-Terminus) - LS-C326889 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ATP5O Antibody - LS-C306445 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-ATP5O Antibody (N-Terminus) - LS-C135184 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ATP5O antibody LS-C135184 Western blot of Mouse Kidney lysate.

Anti-ATP5O Antibody (aa24-213, clone 2E9) - LS-C339232 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2E9
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-ATP5O mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

ATP5O Antibody - NBP2-32602 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

ATP5O Antibody - NBP1-31646 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ATP5O Antibody [NBP1-31646] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.

ATP5O Antibody - H00000539-B01P from Novus Biologicals

Prices $349.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-ATP5O antibody produced in rabbit - SAB2700791 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-ATP5O: <B>Cat. No. SAB2700791</B>: Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using ATP synthase O antibody at 1:100 dilution.

Anti-ATP5O (199-213) antibody produced in rabbit - A1736 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/> HEK-293T cell lysate was separated on SDS-PAGE, blotted with Anti-ATP5O (199-213) (<B>Cat. No. A1736</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500
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