NBN

Gene Symbol NBN
Entrez Gene 4683
Alt Symbol AT-V1, AT-V2, ATV, NBS, NBS1, P95
Species Human
Gene Type protein-coding
Description nibrin
Other Description Nijmegen breakage syndrome 1 (nibrin)|cell cycle regulatory protein p95|p95 protein of the MRE11/RAD50 complex
Swissprots Q53FM6 B2RNC5 B2R626 Q7LDM2 Q32NF7 O60934 Q63HR6 O60672
Accessions AAC62232 AAS59158 AEX07903 AGW81285 AGW81286 AGW81287 AGW81288 AGW81289 AGW81290 AGW81291 BAA28616 CAI45369 EAW91660 EAW91661 O60934 AF051334 AAC39732 AF058696 AAC39752 AK001017 AK223256 BAD96976 AK289848 BAF82537 AK312410 BAG35323 BC005293 BC016762 AAH16762 BC040519 BC071590 BC108650 AAI08651 BC136802 AAI36803 BC136803 AAI36804 BC143065 AAI43066 BC143066 AAI43067 BC144413 BC146797 AAI46798 BX640816 CAH56160 DA634817 XM_005250923 XP_005250980 XM_011517044 XP_011515346 XM_011517045 XP_011515347 XM_011517046 XP_011515348 XR_928335 NM_001024688 NP_001019859 NM_002485 NP_002476
Function Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of
Subcellular Location Nucleus {ECO:0000250}. Nucleus, PML body {ECO:0000269|PubMed:12470659, ECO:0000269|PubMed:15916964}. Chromosome, telomere {ECO:0000250}. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents. {ECO:0000250}.
Tissue Specificity Ubiquitous. Expressed at high levels in testis.
Top Pathways Homologous recombination

Phospho-p95/NBS1 (Ser343) Antibody - 3001 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Mv1Lu cells treated with UV or hydroxyurea (HU) for the indicated times, using Phospho-p95/NBS1 (Ser343) Antibody.

p95/NBS1 Antibody - 3002 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from COS, PC12, NIH3T3, HeLa and Mv1Lu cells, using p95/NBS1 Antibody.

Nibrin (A-2) - sc-374168 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Nibrin (A-2): sc-374168. Western blot analysis of Nibrin expression in Jurkat whole cell lysate.

Nibrin (C-19) - sc-8580 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Nibrin siRNA (h): sc-36061. Western blot analysis of Nibrin expression in non-transfected control (A) and Nibrin siRNA transfected (B) HeLa cells. Blot probed with Nibrin (C-19): sc-8580. α-actinin (H-2): sc-17829 used as specificity and loading control.

Nibrin (H-300) - sc-11431 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Nibrin (H-300): sc-11431. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear and cytoplasmic staining of cells in seminiferous ducts and Leydig cells.

Nibrin (N-19) - sc-8579 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Nibrin (N-19): sc-8579. Western blot analysis of Nibrin expression in non-transfected: sc-117752 (A) and mouse Nibrin transfected: sc-125706 (B) 293T whole cell lysates.

Nibrin (1D7) - sc-56166 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Nibrin (1D7): sc-56166. Western blot analysis of Nibrin expression in HeLa whole cell lysate.

p-Nibrin (Ser 343) - sc-101771 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of phosphorylated Nibrin expression in Jurkat whole cell lysates. Antibodies tested include p-Nibrin (Ser 343): sc-101771 preincubated with cognate phosphorylated peptide (A) and p-Nibrin (Ser 343): sc-101771 (B).

Anti-p95 NBS1 (phospho S278) antibody - ab111373 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-p95 NBS1 (phospho S278) antibody (ab111373) at 1/500 dilutionLane 1 : HUVEC cell extracts, treated with Forskolin (40nM, 30mins)Lane 2 : HUVEC cell extracts, treated with Forskolin (40nM, 30mins) with immunizing peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-p95 NBS1 (phospho S343) antibody - ab47272 from Abcam

Prices $392.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-p95 NBS1 (phospho S343) antibody (ab47272) at 1/500 dilutionLane 1 : 5-30ug Jurkat cell extract, treated with immunogenic peptide.Lane 2 : 5-30ug Jurkat cell extract, untreated.SecondaryAlkaline Phosphatase Goat Anti IgG (H+L)

Anti-p95 NBS1 (phospho S343) antibody - ab195927 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-p95 NBS1 (phospho S343) antibody (ab195927) at 1/500 dilutionLane 1 : Jurkat whole cell lysate with antigen-specific peptideLane 2 : Jurkat whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-p95 NBS1 (phospho S343) antibody [EP178] - ab109453 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP178
All lanes : Anti-p95 NBS1 (phospho S343) antibody [EP178] (ab109453) at 1/100 dilutionLane 1 : Jurkat cell lysate, untreatedLane 2 : Jurkat cell lysate, treated with EtopsideLysates/proteins at 10 µg per lane.

Anti-p95 NBS1 (phospho S432) antibody - ab12297 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Dot blot using ab12297 on phospho and non-phospho peptides corresponding to the immunising peptide sequence.Dots 1: 1ng of peptideDots 2: 10ng of peptideDots 3: 100ng of peptide

Anti-p95 NBS1 (phospho S432) antibody [EPR2470Y] - ab75778 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2470Y
ab75778 staining p95 NBS1 (phospho S432) in Human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/300). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

Anti-p95 NBS1 antibody - ab23996 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-p95 NBS1 antibody (ab23996) at 1/5000 dilutionLane 1 : Lysate from cells engineered to be NBS1 defective (SV40 transformed, immortal fibroblasts)Lane 2 : MRC5 cell lysate (SV40 transformed immortal (WT) fibroblasts)Lysates/proteins at 20 µg per lane.Performed under reducing conditions.

Anti-p95 NBS1 antibody - ab175800 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling p95 NBS1 with ab175800 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. Left - p95 NBS1, Middle - DAPI, Right - Merge.

Anti-p95 NBS1 antibody - ab47386 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-p95 NBS1 antibody (ab47386) at 1/500 dilutionLane 1 : Jurkat cell lysateLane 2 : Jurkat cell lysate with immunogenic peptide (1ug/ml)Lysates/proteins at 30 µg per lane.SecondaryAlkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 0.12 µg/ml

Anti-p95 NBS1 antibody - ab3624 from Abcam

Prices $378.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-p95 NBS1 antibody - ab84488 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC analysis of FFPE section of human breast carcinoma using ab84488 at a dilution of 1/250. Detection: DAB staining.

Anti-p95 NBS1 antibody - ab65133 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-p95 NBS1 antibody (ab65133) at 1/500 dilutionLane 1 : Extracts from HT-29 cellsLane 2 : Extracts from A549 cellsLane 3 : Extracts from A549 cells, plus immunising peptide

Anti-p95 NBS1 antibody [1D7] - ab7860 from Abcam

Prices $379.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 1D7
Overlay histogram showing HeLa cells stained with ab7860 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7860, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-p95 NBS1 antibody [3A9] - ab156997 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3A9
All lanes : Anti-p95 NBS1 antibody [3A9] (ab156997) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with pCMV6-ENTRY p95 NBS1 cDNALysates/proteins at 5 µg per lane.

Anti-p95 NBS1 antibody [7E4A2] - ab181729 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 7E4A2
Immunofluorescent analysis of HeLa cells labeling p95 NBS1 with ab181729 at 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Anti-p95 NBS1 antibody [7E4C2] - ab181780 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 7E4C2
Anti-p95 NBS1 antibody [7E4C2] (ab181780) at 1/500 dilution + Human p95 NBS1 recombinant protein (amino acids 467-615)

Anti-p95 NBS1 antibody [NBS1-501] - ab49958 from Abcam

Prices $373.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone NBS1-501
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