RAPGEF1

Gene Symbol RAPGEF1
Entrez Gene 2889
Alt Symbol C3G, GRF2
Species Human
Gene Type protein-coding
Description Rap guanine nucleotide exchange factor (GEF) 1
Other Description CRK SH3-binding GNRP|guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene)|rap guanine nucleotide exchange factor 1
Swissprots Q68DL3 Q13905 Q5JUE4 Q8IV73
Accessions EAW87985 EAW87986 Q13905 AA761516 AF467069 AAP97718 AJ420417 AK023727 AK023760 AK302763 BAG63974 BC041710 AAH41710 BF058595 BG429001 BG546427 BQ054243 BQ772947 CN360609 CR749354 CAH18207 D21239 BAA04770 DQ925677 ABL59904 DQ925678 ABL59905 DQ925679 ABL59906 DQ925680 ABL59907 DQ925681 ABL59908 DQ925682 ABL59909 DQ925683 ABL59910 DQ925684 ABL59911 EU832841 ACE87852 GQ129245 ACT64379 XM_005272186 XP_005272243 XM_005272191 XP_005272248 XM_006717067 XP_006717130 XM_006717072 XP_006717135 XM_006717074 XP_006717137 XM_011518569 XP_011516871 XM_011518570 XP_011516872 XM_011518571 XP_011516873 XM_011518572 XP_011516874 XM_011518573 XP_011516875 XM_011518574 XP_011516876 XM_011518575 XP_011516877 XM_011518576 XP_011516878 XM_011518577 XP_011516879 XM_011518578 XP_011516880 XM_011518579 XP_011516881 XM_011518580 XP_011516882 XM_011518581 XP_011516883 XM_011518582 XP_011516884 XR_929778 NM_001304275 NP_001291204 NM_005312 NP_005303 NM_198679 NP_941372
Function Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth. {ECO:0000269|PubMed:17724123, ECO:0000269|PubMed:21840392, ECO:0000269|PubMed:7806500}.
Subcellular Location Early endosome {ECO:0000269|PubMed:17724123}.
Tissue Specificity Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.
Top Pathways Focal adhesion, Insulin signaling pathway, Renal cell carcinoma, Neurotrophin signaling pathway, Rap1 signaling pathway

C3G (L-19) - sc-32055 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
C3G (L-19): sc-32055. Western blot analysis of C3G expression in non-transfected: sc-117752 (A) and human C3G transfected: sc-115806 (B) 293T whole cell lysates.

C3G (F-5) - sc-376992 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
C3G (F-5): sc-376992: Western blot analysis of C3G expression in non-transfected HEK 293T (A) and puromycin treated, C3G CRISPR/Cas9 KO Plasmid (h): sc-401616 plus C3G HDR Plasmid (h): sc-401616-HDR co-transfected HEK 293T (B) whole cell lysates. α-actinin (H-2): sc-17829 used as specificity and loading control.

C3G (G-9) - sc-393836 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
C3G (G-9): sc-393836: Western blot analysis of C3G expression in non-transfected HEK 293T (A) and puromycin treated, C3G CRISPR/Cas9 KO Plasmid (h): sc-401616 plus C3G HDR Plasmid (h): sc-401616-HDR co-transfected HEK 293T (B) whole cell lysates. α-actinin (H-2): sc-17829 used as specificity and loading control.

C3G (G-4) - sc-17840 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
C3G (G-4): sc-17840: Western blot analysis of C3G expression in non-transfected HEK 293T (A) and puromycin treated, C3G CRISPR/Cas9 KO Plasmid (h): sc-401616 plus C3G HDR Plasmid (h): sc-401616-HDR co-transfected HEK 293T (B) whole cell lysates. α-actinin (H-2): sc-17829 used as specificity and loading control.

C3G (H-300) - sc-15359 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
C3G (H-300): sc-15359. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tumor tissue showing cytoplasmic localization.

C3G (C-19) - sc-869 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
C3G (C-19): sc-869. Immunofluorescence staining of normal mouse kidney frozen section showing cytoplasmic staining.

p-C3G (Tyr 504) - sc-12926 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of C3G phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) K-562 whole cell lysates. Antibodies tested include p-C3G (Tyr 504)-R: sc-12926-R (A,B) and C3G (H-300): sc-15359 (C,D).

Anti-GRF2 (phospho Y504) antibody - ab74068 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GRF2 (phospho Y504) antibody (ab74068) at 1/500 dilutionLane 1 : Extracts from HepG2 cells treated with Na3VO4 (0.3nM, 40mins),Lane 2 : Extracts from HepG2 cells treated with Na3VO4 (0.3nM, 40mins) with immunising phosphopeptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-GRF2 antibody - ab169279 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Immunofluorescence analysis of HeLa cells, labeling GRF2 with ab169279 at 10&microg/ml.

Anti-GRF2 antibody - ab111061 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab111061 at 1/50 dilution staining GRF2 in paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide.

Anti-GRF2 antibody - ab55157 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab55157 GRF2 antibody at 1ug/ml.

Anti-RAPGEF1 Antibody (clone 2C3) - LS-C338211 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2C3
IHC of paraffin-embedded Human pancreas tissue using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Anti-RAPGEF1 Antibody (clone 2F5) - LS-C338210 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2F5
IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Anti-RAPGEF1 Antibody (aa470-519) - LS-C117988 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RAPGEF1 Antibody (clone 9) - LS-C140112 from LifeSpan Bioscience

Prices $565.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone 9

Anti-RAPGEF1 Antibody (aa50-100) - LS-C288725 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RAPGEF1 Antibody (aa375-425) - LS-C288726 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RAPGEF1 Antibody (aa866-915) - LS-C81919 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.

Anti-RAPGEF1 Antibody (phospho-Tyr504) - LS-C199518 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from HepG2 cells treated with Na3VO4 0.3nM 40', using RapGEF1 (Phospho-Tyr504) Antibody. The lane on the right was incubated with synthetic peptide.

Anti-RAPGEF1 Antibody (clone 1B2) - LS-C338206 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1B2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAPGEF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAPGEF1.

Anti-RAPGEF1 Antibody (clone 3D10) - LS-C197348 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3D10
Western Blot analysis of RAPGEF1 expression in transfected 293T cell line by RAPGEF1 monoclonal antibody (M01), clone 3D10.Lane 1: RAPGEF1 transfected lysate(122.7 KDa).Lane 2: Non-transfected lysate.

Anti-RAPGEF1 Antibody (clone 2B5) - LS-C338212 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2B5
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAPGEF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAPGEF1.

Anti-RAPGEF1 Antibody (clone 1F2) - LS-C338203 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1F2
Anti-RAPGEF1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RAPGEF1.

Anti-RAPGEF1 Antibody (clone 3H5) - LS-C338219 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3H5
Immunofluorescent staining of HeLa cells using anti-RAPGEF1 mouse monoclonal antibody.

GRF2 Antibody - NBP1-88266 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: GRF2 Antibody [NBP1-88266] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
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