SERPINA3

Gene Symbol SERPINA3
Entrez Gene 12
Alt Symbol AACT, ACT, GIG24, GIG25
Species Human
Gene Type protein-coding
Description serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
Other Description alpha-1-antichymotrypsin|cell growth-inhibiting gene 24/25 protein|growth-inhibiting protein 24|growth-inhibiting protein 25|serine (or cysteine) proteinase inhibitor, clade A, member 3|serpin A3
Swissprots Q6LBY8 P01011 Q9UC47 Q6LDT7 Q59GP9 Q9UNU9 B3KVQ7 Q6NSC9 Q13703 Q96DW8 Q8N177 Q2TU88 Q2TU87
Accessions CAA25459 CAA48671 CAI95958 EAW81587 EAW81588 EAW81589 EAW81590 P01011 AB209060 BAD92297 AF089747 AAD08810 AK093049 BAG52641 AK096120 AK123091 BAG53869 AK308565 AY513275 AAT08028 AY513276 AAT08029 BC003559 AAH03559 BC010530 AAH10530 BC013189 AAH13189 BC034554 AAH34554 BC070265 AAH70265 BG565041 CB111903 HQ448280 ADQ32760 J05176 AAA51560 K01500 AAA51543 M18906 AAA51559 M22533 NM_001085 NP_001076
Function Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. {ECO:0000269|PubMed:2404007}.
Subcellular Location Secreted.
Tissue Specificity Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains. {ECO:0000269|PubMed:3257719, ECO:0000269|PubMed:9880565}.

AACT (H-50) - sc-22747 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
AACT (H-50): sc-22747. Western blot analysis of AACT expression in T98G whole cell lysate.

AACT (N-20) - sc-22142 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AACT (N-20): sc-22142. Western blot analysis of AACT expression in non-transfected: sc-117750 (A), human AACT transfected: sc-112327 (B), HeLa (C), U-698-M (D), COLO320DM (E) and U-251-MG (F) whole cell lysates.

AACT (801) - sc-59430 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
AACT (801): sc-59430. Western blot analysis of AACT expression in HeLa (A), U-87 MG (B), T98G (C), U-698-M (D) and SK-N-SH (E) whole cell lysates.

AACT (1.B.734) - sc-69983 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
AACT (1.B.734): sc-69983. Western blot analysis of AACT expression in HeLa whole cell lysate.

AACT (1.B.736) - sc-69982 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
AACT (1.B.736): sc-69982. Western blot analysis of AACT expression in non-transfected 293T: sc-117752 (A), human AACT transfected 293T: sc-112327 (B) and T98G (C) whole cell lysates.

AACT (804) - sc-59432 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
AACT (804): sc-59432. Western blot analysis of AACT expression in non-transfected: sc-117752 (A) and human AACT transfected: sc-112327 (B) 293T whole cell lysates.

AACT (13804) - sc-73432 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
AACT (13804): sc-73432. Western blot analysis of AACT expression in non-transfected: sc-110760 (A) and human AACT transfected: sc-112327 (B) 293 whole cell lysates.

Anti-alpha 1 Antichymotrypsin antibody - ab9374 from Abcam

Prices $376.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
Standard Curve for alpha 1 Antichymotrypsin (Analyte: alpha 1 Antichymotrypsin protein (Human) (ab80517) ); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to alpha 1 Antichymotrypsin (ab54693) at 5µg/ml and Detector Antibody Rabbit polyclonal to alpha 1 Antichymotrypsin (ab9374) at 0.5µg/ml.

Anti-alpha 1 Antichymotrypsin antibody - ab54693 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Standard Curve for alpha 1 Antichymotrypsin (Analyte: alpha 1 Antichymotrypsin protein (Human) (ab80517) ); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to alpha 1 Antichymotrypsin (ab54693) at 5µg/ml and Detector Antibody Rabbit polyclonal to alpha 1 Antichymotrypsin (ab9374) at 0.5µg/ml.

Anti-alpha 1 Antichymotrypsin antibody - ab107362 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-alpha 1 Antichymotrypsin antibody (ab107362) at 1 µg/mlLane 1 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)Lane 2 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051)Lane 3 : Brain Frontal Lobe (Human)Tissue Lysate - AlzheimerLane 4 : Human Plasma Total Protein LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Anti-alpha 1 Antichymotrypsin antibody - ab88527 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-alpha 1 Antichymotrypsin antibody (ab88527) at 1 µg/ml + HeLa cell lysate at 50 µg

Anti-alpha 1 Antichymotrypsin antibody - ab127202 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-alpha 1 Antichymotrypsin antibody (ab127202) at 1 µg/mlLane 1 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)Lane 2 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051)Lane 3 : Human Plasma Total Protein LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-alpha 1 Antichymotrypsin antibody - ab195805 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue labeling alpha 1 Antichymotrypsin with ab195805 at 5 µg/mL.

Anti-alpha 1 Antichymotrypsin antibody - ab49087 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-alpha 1 Antichymotrypsin antibody - ab183081 from Abcam

Host Sheep
Clonality Polyclonal

Anti-alpha 1 Antichymotrypsin antibody [10-118.2] - ab41189 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 10-118.2

Anti-alpha 1 Antichymotrypsin antibody [10-143.1] - ab41190 from Abcam

Prices $370.00
Sizes 500 µg
Host Mouse
Clonality Monoclonal
Clone 10-143.1

Anti-alpha 1 Antichymotrypsin antibody [5G3C11] - C-terminal - ab201987 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5G3C11
Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling alpha 1 Antichymotrypsin with ab201987 at 1/200 dilution.

Anti-alpha 1 Antichymotrypsin antibody [801] - ab20359 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 801
ICC/IF image of ab20359 stained HepG2 cells. The cells were 100% Methanol fixed (5 mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20359, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-alpha 1 Antichymotrypsin antibody [802] - ab20467 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 802

Anti-alpha 1 Antichymotrypsin antibody [804] - ab20821 from Abcam

Host Mouse
Clonality Monoclonal
Clone 804

Anti-alpha 1 Antichymotrypsin antibody [ACT14C7] - ab15606 from Abcam

Prices $370.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal
Clone ACT14C7

Anti-alpha 1 Antichymotrypsin antibody (Biotin) - ab48324 from Abcam

Prices $378.00
Sizes 150 µg
Host Rabbit
Clonality Polyclonal
IHC image of alpha 1 Antichymotrypsin staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab48324, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-alpha 1 Antichymotrypsin antibody [EPR14117(B)] - ab180492 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14117(B)
Anti-alpha 1 Antichymotrypsin antibody [EPR14117(B)] (ab180492) at 1/10000 dilution + Human plasma lysate at 20 µgSecondaryRabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1000 dilution

Anti-alpha 1 Antichymotrypsin antibody [EPR14118(B)] - ab184567 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14118(B)
All lanes : Anti-alpha 1 Antichymotrypsin antibody [EPR14118(B)] (ab184567) at 1/10000 dilutionLane 1 : Human serumLane 2 : Human plasmaLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
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