CAT

Gene Symbol CAT
Entrez Gene 403474
Alt Symbol -
Species Dog
Gene Type protein-coding
Description catalase
Other Description -
Swissprots Q9GKY3 O97492
Accessions O97492 AB012918 BAA36420 AB038231 BAB20764 NM_001002984 NP_001002984
Function Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells.
Subcellular Location Peroxisome {ECO:0000250}.
Top Pathways Amyotrophic lateral sclerosis (ALS), Peroxisome, Glyoxylate and dicarboxylate metabolism, Tryptophan metabolism

Anti-Catalase antibody - ab16731 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot analysis of cell lysates.Lane 1: HeLa cell lysatesLane 2: Jurkat cell lysatesLane 3: Mouse brainLane 4: Rat brainThe band marked with NS is probably non-specific.

Anti-Catalase antibody - ab195306 from Abcam

Prices $372.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Catalase antibody [1B6] - ab125688 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1B6
Overlay histogram showing HeLa cells stained with ab125688 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab125688, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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