MLH1

Gene Symbol MLH1
Entrez Gene 4292
Alt Symbol COCA2, FCC2, HNPCC, HNPCC2, hMLH1
Species Human
Gene Type protein-coding
Description mutL homolog 1
Other Description DNA mismatch repair protein Mlh1|mutL homolog 1, colon cancer, nonpolyposis type 2
Swissprots P40692 E9PCU2 B4DI13 B4DQ11
Accessions AAA77037 AAA82079 AAA85687 AAB80857 AAO22994 AAQ23473 AAQ23474 AAU21566 ABL85063 ABQ45883 ABQ45884 ACR33810 BAA75689 CAR96239 CCQ42976 EAW64483 EAW64484 EAW64485 P40692 AB209848 BAD93085 AF001359 AAB58936 AK222810 BAD96530 AK295359 BAG58325 AK298324 BAG60576 AK298583 BAG60773 AK302807 BAH13807 AK311326 AK311365 AK312609 BAG35497 AK316074 BAH14445 AK316264 BAH14635 AU127758 AY517558 AAT44531 BC006850 AAH06850 BX648844 CN414955 DA517219 DQ648888 ABG49483 DQ648889 ABG49484 DQ648890 ABG49485 DQ648891 ABG49486 DQ648892 ABG49487 DQ648893 ABG49488 EU176254 ABW03705 EU176562 ABW03363 EU188665 ABW69161 EU188666 ABW69162 EU188667 ABW69163 EU188668 ABW69164 EU188669 ABW69165 EU188670 ABW69166 EU188671 ABW69167 EU188672 ABW69168 EU188673 ABW69169 EU188674 ABW69170 EU188675 ABW69171 EU188676 ABW69172 S77856 AAB34135 U07343 AAC50285 U07418 AAA17374 XM_005265161 XP_005265218 XM_005265163 XP_005265220 XM_005265164 XP_005265221 XM_005265166 XP_005265223 XM_011533727 XP_011532029 NM_000249 NP_000240 NM_001167617 NP_001161089 NM_001167618 NP_001161090 NM_001167619 NP_001161091 NM_001258271 NP_001245200 NM_001258273 NP_001245202 NM_001258274 NP_001245203
Function Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS- heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in
Subcellular Location Nucleus {ECO:0000269|PubMed:11429708, ECO:0000269|PubMed:14676842}.
Tissue Specificity Colon, lymphocytes, breast, lung, spleen, testis, prostate, thyroid, gall bladder and heart.
Top Pathways Mismatch repair, Colorectal cancer, Endometrial cancer, Fanconi anemia pathway

MLH1 (D38G9) Rabbit mAb - 4256 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D38G9
Western blot analysis of extracts from various cell types using MLH1 (D38G9) Rabbit mAb.

MLH1 (4C9C7) Mouse mAb - 3515 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 4C9C7
Western blot analysis of extracts from various cell types using MLH1 (4C9C7) Mouse mAb.

MLH1 (B-12) - sc-271978 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MLH1 (B-12): sc-271978. Western blot analysis of MLH1 expression in HeLa nuclear extract.

MLH1 (D-2) - sc-166625 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MLH1 (D-2): sc-166625. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

MLH1 (C-20) - sc-582 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MLH1 (C-20): sc-582. Western blot analysis of MLH1 expression in NIH/3T3 (A), KNRK (B), HeLa (C) and A-431 (D) nuclear extracts.

MLH1 (H-300) - sc-11442 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MLH1 (H-300): sc-11442. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis cancer tissue showing nuclear and cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

MLH1 (N-20) - sc-581 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MLH1 (N-20): sc-581. Western blot analysis of MLH1 expression in NIH/3T3 (A) and KNRK (B) nuclear extracts.

MLH1 (A-8) - sc-133228 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MLH1 (A-8): sc-133228. Western blot analysis of MLH1 expression in A-431 nuclear extract.

MLH1 (Clone 14) - sc-56160 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
MLH1 (Clone 14): sc-56160. Western blot analysis of MLH1 expression in non-transfected 293: sc-110760 (A) and human MLH1 transfected 293: sc-110500 (B) whole cell lysates and MCF7 nuclear extract (C).

MLH1 (G168-15) - sc-56161 from Santa Cruz Biotechnology

Prices $279.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
MLH1 (G168-15): sc-56161. Western blot analysis of MLH1 expression in non-transfected: sc-110760 (A) and human MLH1 transfected: sc-110500 (B) 293 whole cell lysates.

Anti-MLH1 antibody - ab47703 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MLH1 antibody (ab47703) at 1 µg/mlLane 1 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLane 2 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-MLH1 antibody - ab9144 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-MLH1 antibody - ab62729 from Abcam

Prices $372.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MLH1 antibody - ab62732 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MLH1 antibody - ab69628 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MLH1 antibody (ab69628) at 1/500 dilutionLane 1 : Extracts from K562 cells with no immunizing peptideLane 2 : Extracts from K562 cells with with immunizing peptide at 5 µgLysates/proteins at 5 µg per lane.

Anti-MLH1 antibody - ab74541 from Abcam

Prices $376.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal
ab74541 at 1/100 dilution staining MLH1 in human colon carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

Anti-MLH1 antibody [4C9C7] - ab76363 from Abcam

Host Mouse
Clonality Monoclonal
Clone 4C9C7
All lanes : Anti-MLH1 antibody [4C9C7] (ab76363) at 1/500 dilutionLane 1 : Truncated MBP-MLH1 recombinant proteinLane 2 : Truncated Trx-MLH1 (aa381-483) recombinant protein

Anti-MLH1 antibody (Agarose) - ab1295 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MLH1 antibody [EPR3893] - ab108622 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3893
All lanes : Anti-MLH1 antibody [EPR3893] (ab108622) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : Jurkat cell lysateLane 3 : K562 cell lysateLane 4 : SH-SY5Y cell lysateLysates/proteins at 10 µg per lane.

Anti-MLH1 antibody [EPR3894] - ab92312 from Abcam

Prices $403.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3894
All lanes : Anti-MLH1 antibody [EPR3894] (ab92312) at 1/10000 dilutionLane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : A431 cell lysateLane 4 : SW480 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution

Anti-MLH1 antibody [EPR3894] (Alexa Fluor® 405) - ab199495 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3894
ab199495 staining MLH1 in HCT116 cells. The cells were fixed with 4% methanol (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199495 at 1/200 dilution (shown in blue) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8)

Anti-MLH1 antibody [EPR3894] (Alexa Fluor® 647) - ab199494 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3894
ab199494 staining MLH1 in HCT116 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HCT116 cells fixed with 100% methanol (5 min)

Anti-MLH1 antibody [G168-15] - ab14206 from Abcam

Prices $390.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone G168-15
Formalin fixed paraffin embedded human tonsil stained with MLH1 using ABC and DAB chromogen.

Anti-MLH1 antibody [G168-15], prediluted - ab59756 from Abcam

Prices $370.00
Sizes 6 ml
Host Mouse
Clonality Monoclonal
Clone G168-15

Anti-MLH1 antibody, prediluted - ab74540 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
ab74540 staining MLH1 in human colon carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
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