ACTB

Gene Symbol ACTB
Entrez Gene 396526
Alt Symbol Bact, actin
Species Chicken
Gene Type protein-coding
Description actin, beta
Other Description actin, cytoplasmic 1|beta-actin|cytoplasmic 1
Swissprots P60706
Accessions CAA25004 P60706 AJ719605 CAG31264 DQ207609 ABC41741 EU309690 ABY28109 EU931581 ACH68557 FJ891060 ACP21237 GU230784 JN639846 AEQ64020 K02173 AAA98513 L08165 AAA48615 NM_205518 NP_990849
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subcellular Location Cytoplasm, cytoskeleton.
Top Pathways Adherens junction, Tight junction, Focal adhesion, Phagosome, Influenza A

Anti-beta Actin antibody - ab8227 from Abcam

Prices $404.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
IHC image of beta actin staining in a section of formalin-fixed paraffin-embedded [human normal colon]*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was then incubated with ab8227, 1/1000 dilution, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody (ab6720, 1/1000 dilution) was used to detect the primary, and visualized using an HRP conjugated ABC system. Streptavidin HRP was used, ab7403 at a 1/10000 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody

Anti-beta Actin antibody - ab16039 from Abcam

Prices $390.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-beta Actin antibody (ab16039) at 1 µg/mlLane 1 : SK N SH (Human neuroblastoma) Whole Cell LysateLane 2 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Anti-beta Actin antibody - ab199799 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-beta Actin antibody (ab199799) at 1/1000 dilutionLane 1 : SH-SY5Y lysateLane 2 : Human lung lysateSecondarygoat anti-rabbit IgG H&L(HRP) at 1/5000 dilution

Anti-beta Actin antibody - ab70165 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling beta Actin with ab70165 at 1/1000 (1µg/ml). Detection: DAB.

Anti-beta Actin antibody [4C2] - ab123020 from Abcam

Prices $387.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4C2
Overlay histogram showing HeLa cells stained with ab123020 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab123020, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-beta Actin antibody [AC-15] - ab82618 from Abcam

Prices $379.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone AC-15
Overlay histogram showing HeLa cells stained with ab82618 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82618, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse Alexa Fluor® 488 (IgG; H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.

Anti-beta Actin antibody [AC-15] (HRP) - ab49900 from Abcam

Prices $394.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone AC-15
All lanes : Anti-beta Actin antibody [AC-15] (HRP) (ab49900) at 1/50000 dilutionLane 1 : A549 LysateLane 2 : HeLa LysateLane 3 : 3T3 LysateLane 4 : NRK LysateLane 5 : MDCK LysateLane 6 : MDBK LysateLane 7 : BHK LysateLane 8 : COS7 LysateLane 9 : CFB Lysatedeveloped using the ECL technique

Anti-beta Actin antibody [BA3R] - ab92436 from Abcam

Prices $387.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone BA3R
beta Actin was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to beta Actin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab92436.Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.Band: 42kDa; beta Actin

Anti-beta Actin antibody [BA3R] - ab125248 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone BA3R
beta Actin was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to beta Actin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab125248.Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.Band: 42kDa; beta Actin

Anti-beta Actin antibody [BA3R] - ab127418 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone BA3R
All lanes : Anti-beta Actin antibody [BA3R] (ab127418) at 1/1000 dilutionLane 1 : Human tissue lysateLane 2 : Mouse tissue lysateLane 3 : Rat tissue lysateLane 4 : Rabbit tissue lysateLysates/proteins at 20 µg per lane.developed using the ECL technique

Anti-beta Actin antibody [BA3R] (HRP) - ab173838 from Abcam

Prices $376.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone BA3R
All lanes : Anti-beta Actin antibody [BA3R] (HRP) (ab173838) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : U2OS cell lysateLane 4 : COS7 cell lysateLane 5 : NIH 3T3 cell lysateLane 6 : NRK cell lysateLysates/proteins at 30 µg per lane.developed using the ECL technique

Anti-beta Actin antibody [GT5512] - ab184220 from Abcam

Prices $387.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone GT5512
Anti-beta Actin antibody [GT5512] (ab184220) at 1/10000 dilution + Rabbit blood lysate/extract at 30 µg

Anti-beta Actin antibody [mAbcam 8224] - Loading Control - ab8224 from Abcam

Prices $401.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam 8224
IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. A goat anti-mouse HRP-conjugated secondary antibody (ab6789, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-beta Actin antibody [mAbcam 8226] - ab8226 from Abcam

Prices $404.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam 8226
Lanes 1 - 3 : Anti-beta Actin antibody [mAbcam 8226] (ab8226) at 1 µg/ml (5% BSA BLOCK)Lanes 4 - 6 : Anti-beta Actin antibody [mAbcam 8226] (ab8226) at 1 µg/ml (5% MILK BLOCK)Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-beta Actin antibody [mAbcam 8226] (FITC) - ab64496 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone mAbcam 8226
ICC/IF image of ab64496 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab64496, 1µg/ml, FITC conjugated (green)) for 1h at room temperature. 1% BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Anti-beta Actin antibody [mAbcam 8226] - Loading Control (HRP) - ab20272 from Abcam

Prices $394.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone mAbcam 8226
All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (HRP) (ab20272) at 1/5000 dilutionLane 1 : human testis lysateLane 2 : human brain lysateLane 3 : human tonsil lysateLane 4 : mouse brain lysateLane 5 : mouse spleen lysateLysates/proteins at 20 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-beta Actin antibody, prediluted - ab75681 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
Formalin-fixed, paraffin-embedded human smooth muscle (Leiomyoma) stained with ab75681 and AEC chromogen.

Anti-beta Actin antibody [SP124] - ab115777 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone SP124
Flow cytometric analysis of rabbit anti-beta Actin (SP124) antibody ab115777 (1/100) in HeLa cells (green) compared to negative control of rabbit IgG (blue)

Anti-beta Actin antibody - Loading Control - ab8229 from Abcam

Prices $400.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
All lanes : Anti-beta Actin antibody - Loading Control (ab8229) at 1/1000 dilutionLane 1 : HeLa whole cell (Human)Lane 2 : 3T3 cell (Mouse)Lane 3 : Rabbit LiverLane 4 : MDCK cell (Dog) Lane 5 : EBTr cell (Cow)Lane 6 : SL-29 cell (Chicken)Lane 7 : CHO cell (Chinese Hamster)Lysates/proteins at 20 µg per lane.SecondaryRabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-beta Actin antibody - Loading Control - ab13822 from Abcam

Prices $400.00
Sizes 100 µg
Host Chicken
Clonality Polyclonal
All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 3 : Liver (Rat) Tissue LysateLane 4 : CHO-K1 cell lysate Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-beta Actin antibody - N-terminal - ab188589 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue labeling beta Actin with ab188589 at 5 µg/ml.

Anti-beta Actin antibody - N-terminal - ab188645 from Abcam

Prices $376.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded Human tonsil sections labeling beta Actin using ab188645 at 10 µg/ml.

Anti -ACTB (Actin, Cytoplasmic 1, Beta-actin, Actin, Cytoplasmic 1, N-terminallyProcessed) - 137517 from USBiological Life Science

Host Mouse
Clonality Monoclonal
Clone 13H1

Anti -ACTB (Actin, Cytoplasmic 1, Beta-actin, Actin, Cytoplasmic 1, N-terminallyProcessed) - 137518 from USBiological Life Science

Host Rabbit
Clonality Polyclonal

Actin antibody - 10C-CR2001M1 from Fitzgerald

Prices $435.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 103651
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