ETS1

Gene Symbol ETS1
Entrez Gene 396235
Alt Symbol ETS-1, ETS-1A, ETS-1B, EWSR2, TNIP1, c-ets-1, p54
Species Chicken
Gene Type protein-coding
Description v-ets avian erythroblastosis virus E26 oncogene homolog 1
Other Description Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1|TNFAIP3 interacting protein 1|c-ets-1 oncogene|p54 transcription factor|protein C-ets-1|transforming protein p54/c-ets-1|transforming protein p68/c-ets-1|v-ets avian erythroblastosis virus E2 oncogene homolog 1|virion-associated nuclear-shuttling protein
Swissprots P13474 P15062
Accessions AAB29406 P13474 P15062 AY012156 AAG42156 CR353148 CR390578 JQ086323 AEW42989 JQ086324 AEW42990 M21823 AAA48669 X13026 CAA31441 X13027 CAA31442 NM_205298 NP_990629
Function Probable transcription factor. {ECO:0000250}.
Subcellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00237}.
Tissue Specificity Spleen.
Top Pathways Dorso-ventral axis formation

Ets-1 Antibody - NB100-56620 from Novus Biologicals

Prices $99.00, $229.00
Sizes 50 µl, 200 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Ets-1 Antibody [NB100-56620] - Analysis of c-ETS-1 in Jurkat cell lysate using 0.5 ug/ml c-Ets-1 antibody.

Phospho-ETS1 (Ser282) Polyclonal Antibody - 44-1109G from Thermo Fisher Scientific

Prices $309.00
Sizes 10 blots
Host Rabbit
Clonality Polyclonal
Lysates from Jurkat cells either untreated (1, 6), treated with hydrogen peroxide (2-5), or ionophore A23187 (7) were resolved on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 3% milk-TBST buffer for one hour at room temperature, then were incubated with the ETS1[pS 282] antibody overnight at 4%deg;C in a 1% milk-TBST buffer, following prior incubation with: no peptide (1-2, 6-7), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab′)2 anti-rabbit IgG HRP conjugate (Cat. ##ALI4404), and bands were detected using the Pierce SuperSignal™ method.

Phospho-ETS1 (Ser251) Polyclonal Antibody - 44-1107G from Thermo Fisher Scientific

Prices $309.00
Sizes 10 blots
Host Rabbit
Clonality Polyclonal
Peptide Competition: Lysates from Jurkat cells either untreated (1, 6), treated with hydrogen peroxide (2-5), or the ionophore A23187 (7) were resolved on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 3% milk-TBST buffer

Phospho-ETS1 (Thr38) Polyclonal Antibody - 44-1104G from Thermo Fisher Scientific

Prices $304.00
Sizes 10 blots
Host Rabbit
Clonality Polyclonal
Left panel: Extracts of Jurkat cells untreated (1) or treated with 10 µM hydrogen peroxide for 10 minutes (2-5) were resolved on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 3% milk-TBST buffer for one hour at room temperature, then incubated with the ETS1[pT38] antibody overnight at 4°C in a 1% milk-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphothreonine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab') 2 anti-rabbit IgG HRP conjugate (Cat. ##ALI4404) and signals were detected using the Pierce SuperSignal™ method. The data show that only the phosphopeptide corresponding to ETS1[pT38] blocks the signal, verifying the specificity of the antibody. The data also show up-regulation of the signal upon hydrogen peroxide treatment in this cell system. Right panels: Extracts of Jurk
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