RPA1

Gene Symbol RPA1
Entrez Gene 6117
Alt Symbol HSSB, MST075, REPA1, RF-A, RP-A, RPA70
Species Human
Gene Type protein-coding
Description replication protein A1, 70kDa
Other Description MSTP075|RF-A protein 1|RP-A p70|replication factor A protein 1|replication protein A 70 kDa DNA-binding subunit|single-stranded DNA-binding protein
Swissprots Q59ES9 A8K0Y9 P27694
Accessions AAS94324 EAW90574 P27694 AB209732 BAD92969 AF167994 AAQ13598 AK289704 BAF82393 BC018126 AAH18126 HQ258560 ADR83314 M63488 AAA36584 NM_002945 NP_002936
Function As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage (PubMed:17765923). Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Play
Subcellular Location Nucleus {ECO:0000269|PubMed:17959650}. Nucleus, PML body {ECO:0000269|PubMed:17959650}. Note=Enriched in PML bodies in cells displaying alternative lengthening of their telomeres. {ECO:0000269|PubMed:17959650}.
Top Pathways DNA replication, Mismatch repair, Homologous recombination, Nucleotide excision repair, Fanconi anemia pathway

RPA70/RPA1 Antibody - 2267 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from A431 and K562 cells, using RPA70 Antibody.

RPA70/RPA1 (C24F2) Rabbit mAb - 2193 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C24F2
Western blot analysis of extracts from A431 and K562 cells using RPA70 (C24F2) Antibody.

RPA70/RPA1 (4D9) Rat mAb - 2198 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rat
Clonality Monoclonal
Clone 4D9
Western blot analysis of extracts from various cell lines, using RPA70 (4D9) Rat mAb.

RPA 70 kDa subunit (MA70-2) - sc-53497 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
RPA 70 kDa subunit (MA70-2): sc-53497. Western blot analysis of RPA 70 kDa subunit expression in SW480 (A) and K-562 (B) nuclear extracts.

RPA 70 kDa subunit (B-10) - sc-166023 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
RPA 70 kDa subunit (B-10): sc-166023. Western blot analysis of RPA 70 kDa subunit expression in non-transfected: sc-110760 (A) and human RPA 70 kDa subunit transfected: sc-113188 (B) 293 whole cell lysates.

RPA 70 kDa subunit (B-6) - sc-28304 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
RPA 70 kDa subunit (B-6): sc-28304. Western blot analysis of RPA 70 kDa subunit expression in non-transfected: sc-110760 (A) and human RPA 70 kDa subunit transfected: sc-113188 (B) 293 whole cell lysates and A-431 nuclear extract (C).

RPA 70 kDa subunit (C-21) - sc-14696 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RPA 70 kDa subunit (C-21): sc-14696. Western blot analysis of RPA 70 kDa subunit expression in A-431 nuclear extract.

RPA 70 kDa subunit (H-300) - sc-25376 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
RPA 70 kDa subunit (H-300): sc-25376. Western blot analysis of RPA 70 kDa subunit expression in SW480 (A) and K-562 (B) nuclear extracts.

RPA 70 kDa subunit (K-16) - sc-46503 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RPA 70 kDa subunit (K-16): sc-46503. Western blot analysis of RPA 70 kDa subunit expression in A-431 nuclear extract.

RPA 70 kDa subunit (N-19) - sc-46504 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

RPA 70 kDa subunit (H-7) - sc-48425 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
RPA 70 kDa subunit (H-7): sc-48425. Western blot analysis of RPA 70 kDa subunit expression in A-431 nuclear extract.

Anti-RPA70 antibody - ab12320 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma (left) and mouse CT26 colon carcinoma (right) tissues labelling RPA70 with ab12320 at 1/1000 (1 µg/ml). Detection: DAB.

Anti-RPA70 antibody - ab87272 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab87272 at 1/250 dilution staining RPA70 in formalin-fixed, paraffin-embedded Human testicular seminoma tissue. Detection: DAB staining.

Anti-RPA70 antibody - ab97338 from Abcam

Prices $384.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-RPA70 antibody (ab97338) at 1/1000 dilutionLane 1 : H1299 whole cell lysateLane 2 : HeLaS3 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-RPA70 antibody - ab123356 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-RPA70 antibody (ab123356) at 1/100 dilution + K562 cell line lysate at 35 µg

Anti-RPA70 antibody [2H10 or 70C or alphaSSB70C] - ab140212 from Abcam

Prices $378.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone 2H10 or 70C or alphaSSB70C
Anti-RPA70 antibody [2H10 or 70C or alphaSSB70C] (ab140212) at 2 µg/ml + Human Cells-8 Cervical CA HeLa

Anti-RPA70 antibody [4C4] - ab128168 from Abcam

Prices $387.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4C4
ab128168 at 1/10000. Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Anti-RPA70 antibody [8C3-D12-H10] - ab176467 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 8C3-D12-H10
Immunocytochemistry/Immunofluorscence analysis of HeLa cells fixed with paraformaldehyde, labeling RPA70 with ab176467 at 1/100. Left - RPA70, Middle - nuclear staining, Right - Merge.

Anti-RPA70 antibody [EPR3472] - ab79398 from Abcam

Prices $99.00, $397.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3472
All lanes : Anti-RPA70 antibody [EPR3472] (ab79398) at 1/5000 dilutionLane 1 : A549 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-RPA70 antibody [EPR3472] (Alexa Fluor® 488) - ab199097 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3472
ab199097 staining RPA70 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199097 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-RPA70 antibody [EPR3472] (Alexa Fluor® 647) - ab199240 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3472
ab199240 staining RPA70 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199240 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

Anti-RPA70 antibody [EPR3473] - ab133364 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3473
All lanes : Anti-RPA70 antibody [EPR3473] (ab133364) at 1/10000 dilutionLane 1 : A431 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-RPA70 antibody [RPA15I373] - ab50936 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone RPA15I373
Anti-RPA70 antibody [RPA15I373] (ab50936) at 1/50 dilution + HeLa whole cell lysate at 25 µgSecondaryMouse IgG antibody at 1/2500 dilution

Anti-RPA70 / RPA1 Antibody - LS-C38949 from LifeSpan Bioscience

Prices $455.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal

Anti-RPA70 / RPA1 Antibody (aa450-462) - LS-C38950 from LifeSpan Bioscience

Prices $325.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
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