HIST1H2AE

Gene Symbol HIST1H2AE
Entrez Gene 3012
Alt Symbol H2A.1, H2A.2, H2A/a, H2AFA
Species Human
Gene Type protein-coding
Description histone cluster 1, H2ae
Other Description H2A histone family, member A|histone 1, H2ae|histone H2A type 1-B/E|histone H2A/a|histone H2AE
Accessions AAA63191 AAN59967 EAW55546 P04908 AK311824 BAG34766 BC093836 AAH93836 BC093862 AAH93862 EU446964 ABZ92493 NM_021052 NP_066390
Top Pathways Systemic lupus erythematosus, Alcoholism

Human Histone H2A (biotinylated ) full length protein - ab200286 from Abcam

Category Protein
Source E. coli
Species Human
Lane 1: 4-20% SDS-PAGE Coomassie staining using 2 µg ab200286 Lane 2: Marker

Human Histone H2A (di methyl K95) peptide - ab10127 from Abcam

Category Peptide
Species Human
Lanes 1 & 3 : Anti-Histone H2A (di methyl K95) antibody ( ab4633 ) at 1/500 dilution Lanes 2 & 4 : Anti-Histone H2A (di methyl K95) antibody ( ab4633 ) at 1/1000 dilution Lane 1 : 1ug of calf thymus Histone prep Lane 2 : 1ug of calf thymus Histone prep Lane 3 : 1ug of calf thymus Histone prep with Human Histone H2A (di methyl K95) peptide (ab10127) at 2 µg Lane 4 : 1ug of calf thymus Histone prep with Human Histone H2A (di methyl K95) peptide (ab10127) at 2 µg Secondary Lanes 1 - 3 : Goat Anti-Rabbit IgG H&L (HRP) ( ab6721 ) at 2000 Lane 4 : Goat Anti-Rabbit IgG H&L (HRP) ( ab6721 ) at 1/2000 dilution

Human Histone H2A (di methyl K99) peptide - ab10125 from Abcam

Category Peptide
Species Human

Human Histone H2A (glcnac T101) peptide - ab187249 from Abcam

Category Peptide
Species Human

Human Histone H2A (hydroxy P26) peptide - ab32016 from Abcam

Category Peptide
Species Human
ELISA eluate (red, blue) and flow through (green) using immunizing peptide (ab12952) . Eluate (orange) and flow through (grey) using un-modified peptide (ab13202) . This demonstrates that ab12950 specifically recognises mono-methyl K127 compared to the unmodified peptide.

Human Histone H2A (symmetric di methyl R3) peptide - ab22399 from Abcam

Category Peptide
Species Human

Human Histone H2A (tri methyl K127) peptide - ab24659 from Abcam

Category Peptide
Species Human

Human Histone H2A (unmodified ) peptide - ab13186 from Abcam

Category Peptide
Source CHO
ELISA using ab17346 . The blue line indicates binding to the H2A phospho S1 immunising peptide ( ab17590 ). Binding to a peptide with an unmodified S1 residue (ab13186) was greatly reduced (red line). This indicates that ab17346 is specific for the modified peptide.
ELISA eluate (red, blue) and flow through (green) using immunizing peptide (ab12952) . Eluate (orange) and flow through (grey) using un-modified peptide (ab13202) . This demonstrates that ab12950 specifically recognises mono-methyl K127 compared to the unmodified peptide.

Human Histone H2A (unmodified ) peptide - ab22430 from Abcam

Category Peptide
Species Human

Human Histone H2A full length protein - ab132918 from Abcam

Category Protein
Source Wheat germ
Species Human
12.5% SDS-PAGE analysis of ab132918 stained with Coomassie Blue.

Human Histone H2A full length protein - ab132930 from Abcam

Category Protein
Source Wheat germ
Species Human
12.5% SDS-PAGE analysis of ab132930 stained with Coomassie Blue.

Human Histone H2A full length protein - ab159994 from Abcam

Category Protein
Source Wheat germ
Species Human
ab159994 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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