ab2770 staining aryl hydrocarbon receptor in MDA-MB-231 cells treated with nifedipine (ab120135), by ICC/IF. Increase in aryl hydrocarbon receptor expression correlates with increased concentration of nifedipine, as described in literature.The cells were incubated at 37°C for 6h in media containing different concentrations of ab120135 (nifedipine) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2770 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

ab2770 staining aryl hydrocarbon receptor in MDA-MB-231 cells treated with nifedipine (ab120135), by ICC/IF. Increase in aryl hydrocarbon receptor expression correlates with increased concentration of nifedipine, as described in literature.The cells were incubated at 37°C for 6h in media containing different concentrations of ab120135 (nifedipine) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2770 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.


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