All lanes : Anti-eIF3e antibody ( ab36766 ) at 1 µg/ml Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate ( ab7899 ) Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate ( ab7909 ) Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate ( ab7902 ) Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human eIF3e peptide (ab36902) at 1 µg/ml Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate ( ab7899 ) with Human eIF3e peptide (ab36902) at 1 µg/ml Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate ( ab7909 ) with Human eIF3e peptide (ab36902) at 1 µg/ml Lane 8 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate ( ab7902 ) with Human eIF3e peptide (ab36902) at 1 µg/ml Lysates/proteins at 10 µg per lane. Secondary IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution