All lanes : Anti-FOXO1A (phospho S256) antibody ( ab26651 ) at 1 µg/ml Lane 1 : Ovary (Human) Tissue Lysate Lane 2 : Ovary (Human) Tissue Lysate with Human FOXO1A (phospho S256) peptide (ab27886) at 1 µg/ml Lane 3 : Ovary (Human) Tissue Lysate with Human FOXO1A peptide ( ab27887 ) at 1 µg/ml Lysates/proteins at 20 µg per lane. Secondary IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution Performed under reducing conditions. Predicted band size : 70 kDa Observed band size : 70 kDa Additional bands at : 53 kDa. We are unsure as to the identity of these extra bands.