Active E. coli BirA full length protein
| Name | Active E. coli BirA full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab135015 |
| Category | Protein |
| Prices | $528.00 |
| Sizes | 100 µg |
| Applications | WB FA SDS-PAGE |
| Species Reactivities | Escherichia coli |
| Nature | Recombinant |
| Source | E. coli |
| Purity | >= 65 % by SDS-PAGE. |
| Bioactivity | ab135015 (10 µg/ml) completely biotinylated 10 µM of substrate MBP-Avi at 30°C for 30 minutes in the standard reaction buffer containing 50 mM Bicine pH 8.3, 10 mM ATP, 10 mM MgOAc, and 50 µM d-Biotin. |
| SwissProt/Accession | P06709 |
| Gene | birA |
| Residue | 2 to 321 |
| Sequence | KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVF TVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGEL KSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSL VIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQI VIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALE LFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLE QDGIIKPWMGGEISLRSAEK |
| Supplier Page | Shop |
Product images
MBP-Avi biotinylation with His-FLAG-BirA using the standard reaction conditions as outlined in Enzyme Activity. A. Coomassie blue stained gel showing 1. unbiotinylated MBP-Avi 2. His-FLAG-BirA / MBPAvi 3. MBP-Avi-biotin control B. The corresponding Western blot probed with Streptavidin-HRP and developed with DAB (3,3’-Diaminobenzidine) substrate.
10% SDS-PAGE analysis of ab135015 (4 µg) stained with Coomassie Blue.