birA

Gene Symbol birA
Entrez Gene 948469
Alt Symbol ECK3965, JW3941, bioR, dhbB
Species Escherichia coli
Gene Type protein-coding
Description bifunctional biotin-[acetylCoA carboxylase] holoenzyme synthetase/ DNA-binding transcriptional repressor, bio-5'-AMP-binding
Other Description -
Swissprots Q2M8R4 P06709
Accessions AAC43075 AAC76951 NP_418404
Function Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio- 5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon. {ECO:0000255|HAMAP- Rule:MF_00978, ECO:0000269|PubMed:12527300, ECO:0000269|PubMed:2667763, ECO:0000269|PubMed:6129246, ECO:0000269|PubMed:8003500}.
Top Pathways Biotin metabolism

Active E. coli BirA full length protein - ab135015 from Abcam

Category Protein
Prices $528.00
Sizes 100 µg
Source E. coli
Species Escherichia coli
MBP-Avi biotinylation with His-FLAG-BirA using the standard reaction conditions as outlined in Enzyme Activity. A. Coomassie blue stained gel showing 1. unbiotinylated MBP-Avi 2. His-FLAG-BirA / MBPAvi 3. MBP-Avi-biotin control B. The corresponding Western blot probed with Streptavidin-HRP and developed with DAB (3,3’-Diaminobenzidine) substrate.

E. coli BirA full length protein - ab198449 from Abcam

Category Protein
Prices $858.00
Sizes 100 µg
Source E. coli
Species Escherichia coli
Image A: SDS-PAGE with Coomassie blue showing: Lane 1: Unbiotinylated BirA Lane 2: ab198449 Lane 3: BRD4-Avi-biotin control Lane 4: Protein markers Image B. Shows the corresponding Western blot probed with Streptavidin-HRP and developed with ECL reagent.

E. coli BirA full length protein - ab198454 from Abcam

Category Protein
Prices $858.00
Sizes 50 µg
Source Baculovirus infected sf9 cells
Species Escherichia coli
4-20% SDS-PAGE analysis of 3.3 µg ab198454 with Coomassie staining.

E.coli BirA protein (MBP Tag) - orb138259 from Biorbyt

Category Protein
Prices $400.00, $612.00
Sizes 50 μg, 100 μg
Source E. coli
SDS-PAGE analysis of E.coli BirA protein (MBP Tag)

Recombinant Escherichia coli Bifunctional protein BirA(birA) - CSB-YP361925ENV CSB-EP361925ENV CSB-BP361925ENV CSB-MP361925ENV from Cusabio

Category Protein
Source Yeast or E.coli or Baculovirus or Mammalian cell
Species Escherichia coli

Bifunctional protein BirA (birA), Recombinant Protein - MBS1053823 from MyBioSource

Category Protein
Prices $1,080.00, $1,080.00, $1,310.00, $1,310.00, $1,695.00, $1,695.00, $2,160.00, $2,160.00
Sizes 500 µg (E-Coli), 50 µg (Baculovirus), 500 µg (Yeast), 50 µg (Mammalian-Cell), 1 mg (E-Coli), 100 µg (Baculovirus), 1 mg (Yeast), 100 µg (Mammalian-Cell)
Source E Coli or Yeast or Baculovirus or Mammalian Cell
Species Escherichia coli
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