Active human PAK2 protein fragment
| Name | Active human PAK2 protein fragment |
|---|---|
| Supplier | Abcam |
| Catalog | ab95310 |
| Category | Protein |
| Prices | $761.00 |
| Sizes | 10 µg |
| Applications | FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Purity | > 90 % by SDS-PAGE. |
| Bioactivity | 86 nmoles/min/µg Analysis of enzymatic activity: 1. Different concentrations of PAK2 were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 20) at room temperature for 1 hour. 2. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT. 3. Fluorescence was detected using ?exc=460±40 nm and ?em=528±20 nm filters. |
| SwissProt/Accession | Q13177 |
| Gene | PAK2 |
| Residue | 227 to 524 |
| Sequence | Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CRIB domain. Contains 1 protein kinase domain. |
| Supplier Page | Shop |
