All lanes : Anti-SP1 (phospho T453) antibody ( ab37707 ) at 1 µg/ml Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SP1 (phospho T453) peptide (ab38350) at 1 µg/ml Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human SP1 (phospho T453) peptide (ab38350) at 1 µg/ml Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SP1 peptide ( ab42654 ) at 1 µg/ml Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human SP1 peptide ( ab42654 ) at 1 µg/ml Lysates/proteins at 10 µg per lane. Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution developed using the ECL technique Performed under reducing conditions. Predicted band size : 81 kDa Observed band size : 120 kDa ( why is the actual band size