| Name | Ubiquitin protein (Mutated K33) (His tag), Chemical Free |
|---|---|
| Supplier | Abcam |
| Catalog | ab80742 |
| Category | Protein |
| Applications | Conjugation SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Tag/Conjugation | His tag N-Terminus |
| Purity | > 95 % by SDS-PAGE. |
| Bioactivity | Mutation of lysine 33 to arginine renders ubiquitin (Ub) unable to form poly-Ub chains via lysine 33 linkages with other Ub molecules. ab80742 can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins. Ideal for the reduction in poly-Ub chain length/conjugation rates and determining if poly-Ub chains are K33 linked. |
| SwissProt/Accession | P0CG47 |
| Gene | UBB |
| Supplier Page | Shop |