Ubiquitin protein (Mutated K48) (His tag), Chemical Free

• Supplier: Abcam
• Catalog: ab80740
• Category: Protein
• Applications: Conjugation SDS-PAGE
• Species Reactivities: Human
• Nature: Recombinant
• Source: E. coli
• Tag/Conjugation: His tag N-Terminus
• Purity: > 95 % by SDS-PAGE.
• Bioactivity: Mutation of lysine 48 to arginine renders ubiquitin (Ub) unable to form poly-Ub chains via lysine 48 linkages with other Ub molecules. ab80740 can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins (monoubiquitination). Ideal for the reduction in poly-Ub chain length/conjugation rates and for the determination of poly-Ub chains specificity.
• SwissProt/Accession: P62988
• Gene: UBC