| Name |
Active human B Raf protein fragment
|
| Supplier |
Abcam
|
| Catalog |
ab196139
|
| Category |
Protein
|
| Prices |
$612.00
|
| Sizes |
10 µg
|
| Applications |
FA SDS-PAGE
|
| Species Reactivities |
Human
|
| Nature |
Recombinant
|
| Source |
Baculovirus infected sf9 cells
|
| Tag/Conjugation |
GST tag N-Terminus
|
| Purity |
>= 35 % by SDS-PAGE.
|
| Bioactivity |
Specific Activity: 44 pmol/min/µg Assay Conditions: Reaction was performed in a kinase buffer (40 mM Tris, 10 mM MgCl2, 0.1 mg/ml BSA) containing 2 μg inactive MEK1, 5 μM ATP, and 0.1 – 1.6 ng/μl BRAF for 60 minutes at room temperature. The amount of ATP transferred was measured by BPS ATP quantification reagents.
|
| SwissProt/Accession |
P15056
|
| Gene |
BRAF
|
| Residue |
417 to 766
|
| Sequence |
QKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSG SFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLF MGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDY LHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGS ILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQII FMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIEL LARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH
|
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