All lanes : Anti-BMP4 antibody ( ab39973 ) at 1 µg/ml Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue ( ab29466 ) Lane 2 : Brain (Mouse) Mouse Tissue Lysate Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue ( ab29889 ) Lane 4 : Heart (Human) Tissue Lysate - adult normal tissue ( ab29431 ) Lane 5 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue ( ab29330 ) Lane 6 : Brain (Human) Tissue Lysate - adult normal tissue ( ab29466 ) with Human BMP4 peptide (ab40140) at 1 µg/ml Lane 7 : Brain (Mouse) Mouse Tissue Lysate with Human BMP4 peptide (ab40140) at 1 µg/ml Lane 8 : Liver (Human) Tissue Lysate - adult normal tissue ( ab29889 ) with Human BMP4 peptide (ab40140) at 1 µg/ml Lane 9 : Heart (Human) Tissue Lysate - adult normal tissue ( ab29431 ) with Human BMP4 peptide (ab40140) at 1 µg/ml Lane 10 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue ( ab29330 ) with Human BMP4 peptide (ab40140) at 1 µg/ml Lysates/proteins at 10 µg per lane. Secondary