Active human FGF basic full length protein
| Name | Active human FGF basic full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab9596 |
| Category | Protein |
| Prices | $110.00, $257.00 |
| Sizes | 10 µg, 50 µg |
| Applications | Cell differentiation SDS-PAGE FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Purity | > 95 % by SDS-PAGE. Sterile filtered Greater than 95% pure by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg). |
| Bioactivity | active |
| Endotoxin | < 0.100 Eu/µg |
| SwissProt/Accession | P09038 |
| Gene | FGF2 |
| Sequence | Human FGF-basic is a 17.2 kDa protein containing 154 amino acid residues: MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHIKLQLQAEERG VVSIKG VCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKYTS WYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS |
| Supplier Page | Shop |
Product images
Human embryonic stem cells (H9) were differentiated both as embryoid bodies and monolayer cultures (on Matrigel) using a protocol described in Kennedy et al., 2007. BMP-4, FGF-basic (ab9596) and VEGF ( ab9571 ) were sequentially added to the media to direct differentiation. These images were taken at day 8, where cell adhesion antigens CD31 ( ab32457 in green) and CD34 ( ab8536 in red) are abundant and represent primitive haematopoietic cells within the culture. Dapi staining is shown in blue.
![Standard Curve for FGF basic (Analyte: FGF basic protein (Active) (ab9596) ); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [AS24] to FGF basic (ab17279) at 1ug/ml and Detector Antibody Rabbit monoclonal [EP1735] to FGF basic (ab92337) at 0.5ug/ml.](http://www.bioprodhub.com/system/product_images/pp_products/2/sub_1/760_FGF-basic-Proteins-and-Peptides-ab9596-2.jpg)
Standard Curve for FGF basic (Analyte: FGF basic protein (Active) (ab9596) ); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [AS24] to FGF basic (ab17279) at 1ug/ml and Detector Antibody Rabbit monoclonal [EP1735] to FGF basic (ab92337) at 0.5ug/ml.