Flt3 / CD135 protein (Fc Chimera Active)
| Name | Flt3 / CD135 protein (Fc Chimera Active) |
|---|---|
| Supplier | Abcam |
| Catalog | ab83998 |
| Category | Protein |
| Prices | $302.00 |
| Sizes | 25 µg |
| Applications | SDS-PAGE FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | HEK 293 cells |
| Purity | > 95 % by SDS-PAGE. |
| Bioactivity | Activity: The ED 50 of ab83998 is typically 1.0 -2.0 ng/ml as measured by its ability to neutralize Flt3 mediated proliferation of the OCI/AML5 human leukemia cell line. |
| SwissProt/Accession | P36888 |
| Gene | FLT3 |
| Sequence | Theoretical Sequence: NQDLPVIKCVLINHKNNDSSVGKSSSYPMVSESPEDLGC ALRPQSSGTVYEAAAVEVDVSASITLQVL VDAPGNISCLWVFKHSSLN CQPHFDLQNRGVVSMVILKMTETQAGEYLLFIQSEATNYTILFTVSIRNT L LYTLRRPYFRKMENQDALVCISESVPEPIVEWVLCDSQGESCKEESP AVVKKEEKVLHELFGTDIRCC ARNELGRECTRLFTIDLNQTPQTTLPQ LFLKVGEPLWIRCKAVHVNHGFGLTWELENKALEEGNYFEM STYSTNR TMIRILFAFVSSVARNDTGYYTCSSSKHPSQSALVTIVEKGFINATNSSE DYEIDQYEEFCFS VRFKAYPQIRCTWTFSRKSFPCEQKGLDNGYSISK FCNHKHQPGEYIFHAENDDAQFTKMFTLNIRRK PQVLAEASASQASCF SDGYPLPSWTWKKCSDKSPNCTEEITEGVWNRKANRKVFGQWVSSSTLNM SEAIKGFLVKCCAYNSLGTSCETILLNSPGPFPFIQDRIPKVDKKVEP KSCDKTHTCPPCPAPELLGGPS VFLFPPKPKDTLMISRTPEVTCVVVD VSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVL TVLHQDW LNGKEYKCRVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQV SLTCLVKGFYP SDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLT VDKSRWQQGNVFSCSVMHEALHNHYTQKS LSLSPGK |
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Product images
Densitometry of protein isoforms visualised by 2-DE. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.
1D SDS-PAGE of ab83998 before and after treatment with glycosidases to remove oligosaccharides. Lane 1: ab83998 Lane 2: ab83998 treated with PNGase F to remove potential N-linked glycans Lane 3: ab83998 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 µg protein loaded per lane; Deep Purple™ stained.
A sample of ab83998 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 µg protein loaded. Spot train indicates presence of multiple isoforms.