EIA Enzyme immunoassay



Anti-11 alpha Hydroxytestosterone antibody - ab54043 from Abcam

Prices $370.00
Sizes 150 µg
Host Rabbit
Clonality Polyclonal

Anti-11-Ketotestosterone antibody - ab54042 from Abcam

Prices $370.00
Sizes 150 µg
Host Rabbit
Clonality Polyclonal

Anti-17 alpha Estradiol antibody - ab59672 from Abcam

Host Sheep
Clonality Polyclonal

Anti-17 beta Estradiol 3 antibody - ab50660 from Abcam

Prices $370.00
Sizes 100 µl
Host Goat
Clonality Polyclonal

Anti-4-Pregnen-17, 20 beta- Diol-3-One antibody - ab53950 from Abcam

Prices $370.00
Sizes 150 µg
Host Rabbit
Clonality Polyclonal

Anti-Adiponectin antibody (Biotin) - ab51420 from Abcam

Prices $370.00
Sizes 150 µg
Host Rabbit
Clonality Polyclonal

Anti-Adrenorphin antibody - ab53958 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Albendazole antibody - ab58929 from Abcam

Host Sheep
Clonality Polyclonal

Anti-Allatostatin 1 antibody - ab53956 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of Birgus latro (Coconut crab) brain tissue, staining Allatostatin 1 (green) with ab53956. The brains were dissected in phosphate buffered saline (0.1 M PBS, pH 7.4) then fixed in 4% PFA in 0.1 PBS, pH 7.4 at 4°C before sectioning at room temperature. The sections were incubated for 90 minutes in PBS-TX (1% BS|A, 0.3% Triton X-100, 0.05% sodium azide, pH 7.4) followed by overnight incubation with primary antibody (1/1000 in PBS-TX) at room temperature. An AlexaFluor®488-conjugated secondary antibody was used to detect staining.

Anti-Allatostatin 2 antibody - ab53955 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Allatostatin 4 antibody - ab53953 from Abcam

Prices $370.00
Sizes 150 µg
Host Rabbit
Clonality Polyclonal

Anti-Allatostatin 4 antibody (Biotin) - ab48618 from Abcam

Prices $370.00
Sizes 150 µg
Host Rabbit
Clonality Polyclonal

Anti-Allatostatin 8 antibody - ab53954 from Abcam

Prices $370.00
Sizes 150 µg
Host Rabbit
Clonality Polyclonal

Anti-alpha 1 Adrenergic Receptor antibody - ab3462 from Abcam

Prices $398.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of alpha 1 Adrenergic Receptor (green) showing staining in the cytoplasm of HepG2 cells (right) compared to a negative control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3462 in 3% BSA-PBS at a dilution of 1:100 overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Anti-Androstanedione antibody - ab58931 from Abcam

Host Sheep
Clonality Polyclonal

Anti-Azurocidin antibody (Biotin) - ab51508 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-C3 antibody - ab48611 from Abcam

Prices $379.00
Sizes 150 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab48611 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab48611, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-C4 antibody - ab48612 from Abcam

Prices $372.00
Sizes 150 µg
Host Rabbit
Clonality Polyclonal

Anti-Catalase antibody - ab48613 from Abcam

Prices $378.00
Sizes 150 µg
Host Rabbit
Clonality Polyclonal
IHC image of Catalase staining in Human Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab48613, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Ceruloplasmin antibody - ab48614 from Abcam

Prices $384.00
Sizes 150 µg
Host Rabbit
Clonality Polyclonal
Ab48614 staining human tonsil. Staining is localized to the cytoplasm.Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Ceruloplasmin antibody (Biotin) - ab48650 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Clostebol antibody - ab59662 from Abcam

Host Sheep
Clonality Polyclonal

Anti-Collagen VII antibody - ab93350 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical staining of Collagen VII in human skin tissue sections with ab93350. Samples were fixed with formaldehyde and enzymatic antigen retrieval was performed with Proteinase K. Tissue was blocked with 1% BSA for 10 minutes at 21°C before ab93350 was added (1/250) for 2 hours at 21°C. A biotin conjugated goat anti-rabbit secondary was used at 1/250.See Abreview

Anti-Cortexolone antibody - ab48593 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Cpn10 antibody [M1.2] - ab13527 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone M1.2
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