mTOR signaling pathway



SignalSilence ® Akt siRNA I - 6211 from Cell Signaling Technology

Category siRNA
Prices $256.00
Sizes 300 µl (3 nmol)
Species Reactivities Human
Western blot analysis of extracts from CHO cells, transfected with non-targeted (-) or SignalSilence ® Akt siRNA I (+) siRNA, using Akt Antibody #9272 and p42 MAP Kinase (Erk2) Antibody #9108. The Akt antibody confirms silencing of protein expression while the p42 MAP Kinase (Erk2) antibody was used to control for loading and specificity of Akt siRNA (A). Phospho-GSK-3β (Ser9) Antibody #9336 was used to confirm downstream pathway inhibition (B).

SignalSilence ® Akt siRNA II - 6510 from Cell Signaling Technology

Category siRNA
Prices $256.00
Sizes 300 µl (3 nmol)
Species Reactivities Human
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence ® Akt siRNA II (+), using Akt (pan) (11E7) Rabbit mAb #4685 and β-Actin (13E5) Rabbit mAb #4970. Akt (pan) (11E7) Rabbit mAb confirms silencing of Akt expression, while β-Actin (13E5) Rabbit mAb is used to control for loading and specificity of Akt siRNA.

Akt1 CRISPR/Cas9 KO Plasmid (h) - sc-400014 from Santa Cruz Biotechnology

Category CRISPR
Prices $345.00
Sizes 20 µg
Species Reactivities Human

Akt1 HDR Plasmid (h) - sc-400014-HDR from Santa Cruz Biotechnology

Prices $345.00
Sizes 20 µg
Species Reactivities Human

Akt1 Double Nickase Plasmid (h) - sc-400014-NIC from Santa Cruz Biotechnology

Prices $345.00
Sizes 20 µg
Species Reactivities Human

Akt1 Double Nickase Plasmid (h2) - sc-400014-NIC-2 from Santa Cruz Biotechnology

Prices $345.00
Sizes 20 µg
Species Reactivities Human

Akt1 CRISPR Activation Plasmid (h) - sc-400014-ACT from Santa Cruz Biotechnology

Category CRISPR
Prices $345.00
Sizes 20 µg
Species Reactivities Human

Akt1 CRISPR Activation Plasmid (h2) - sc-400014-ACT-2 from Santa Cruz Biotechnology

Category CRISPR
Prices $345.00
Sizes 20 µg
Species Reactivities Human

Akt1 Lentiviral Activation Particles (h) - sc-400014-LAC from Santa Cruz Biotechnology

Prices $345.00
Sizes 20 µg
Species Reactivities Human

Akt1 Lentiviral Activation Particles (h2) - sc-400014-LAC-2 from Santa Cruz Biotechnology

Prices $345.00
Sizes 20 µg
Species Reactivities Human

Akt1 siRNA (h) - sc-29195 from Santa Cruz Biotechnology

Category siRNA
Prices $258.00
Sizes 10 µm
Species Reactivities Human

Akt1 shRNA Plasmid (h) - sc-29195-SH from Santa Cruz Biotechnology

Category siRNA
Prices $258.00
Sizes 10 µm
Species Reactivities Human

Akt1 shRNA (h) Lentiviral Particles - sc-29195-V from Santa Cruz Biotechnology

Category siRNA
Prices $258.00
Sizes 10 µm
Species Reactivities Human

Akt1/2 siRNA (h) - sc-43609 from Santa Cruz Biotechnology

Category siRNA
Prices $258.00
Sizes 10 µm
Species Reactivities Human

Akt1/2 shRNA Plasmid (h) - sc-43609-SH from Santa Cruz Biotechnology

Category siRNA
Prices $258.00
Sizes 10 µm
Species Reactivities Human

Akt1/2 shRNA (h) Lentiviral Particles - sc-43609-V from Santa Cruz Biotechnology

Category siRNA
Prices $258.00
Sizes 10 µm
Species Reactivities Human

Akt1 siRNA (h2) - sc-44198 from Santa Cruz Biotechnology

Category siRNA
Prices $258.00
Sizes 10 µm
Species Reactivities Human

Akt1 shRNA Plasmid (h2) - sc-44198-SH from Santa Cruz Biotechnology

Category siRNA
Prices $258.00
Sizes 10 µm
Species Reactivities Human

Akt1 shRNA (h2) Lentiviral Particles - sc-44198-V from Santa Cruz Biotechnology

Category siRNA
Prices $258.00
Sizes 10 µm
Species Reactivities Human

AKT1 RNAi - H00000207-R02V from Novus Biologicals

Category siRNA
Prices $399.00
Sizes 20 nmol
Species Reactivities Human
Western Blot: Akt1 RNAi [H00000207-R02V] - Analysis of AKT1 over-expressed 293 cell line. Lane 2 cotransfected with AKT1 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00000207-M01. GAPDH ( 36.1 kDa ) used as loading control.

AKT1 RNAi - H00000207-R08 from Novus Biologicals

Category siRNA
Prices $399.00
Sizes 20 nmol
Species Reactivities Human

Akt1 RNAi - H00000207-R01V from Novus Biologicals

Category siRNA
Prices $629.00
Sizes 20 nmol
Species Reactivities Human
Western blot analysis of AKT1 over-expressed 293 cell line. Lane 2 cotransfected with AKT1 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00000207-M01. GAPDH ( 36.1 kDa ) used as loading control.

MISSION® esiRNA - EHU083501 from Sigma-Aldrich

Category siRNA
Prices $140.00, $250.00
Sizes 20 µg, 50 µg
<B>Immunoblotting</B><BR/>AKT1: <B>Cat. No. EHU083501</B>: Western blot analysis of AKT1 in HeLa cells.<BR/><B>Lanes</B><BR/>1. HeLa cells transfected with Renilla Luciferase esiRNA (RLUC, <B>Cat. No. EHURLUC</B>) as negative control.<BR/>2. HeLa cells transfected with AKT1 esiRNA (<B>Cat. No. EHU083501</B>). Quantitative-Immunoblotting: Lysates were separated on SDS-Page and probed with mouse Monoclonal Anti-Protein Kinase Bα (1:5000) Clone: PKB-175 (<B>Cat. No. P2482</B>) and goat Anti-GAPDH (1:7000) (Acris) antibodies. The signal intensities of indicated bands were quantified using an Odyssey infrared imaging system (Li-COR) and normalized to the GAPDH signal. At 72h post transfection, an 88% AKT1 protein reduction was measured between the negative control RLUC and the sample treated with AKT1 esiRNA.

MISSION® siRNA - SIHK0096 from Sigma-Aldrich

Category siRNA
Prices $25.00
Sizes 0.25 nmol

MISSION® siRNA - SIHK0095 from Sigma-Aldrich

Category siRNA
Prices $25.00
Sizes 0.25 nmol
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