Wnt signaling pathway



APC Antibody - 2504 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis (lane 1) and Immunoprecipitation (lanes 2-3) of extracts from SW480 cells. APC was immunoprecipitated with APC Antibody (lane 2) or with rabbit IgG (lane 3) to confirm specificity. Western blot detection was performed using APC Antibody.

APC (A-3) - sc-393704 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
APC (A-3): sc-393704. Western blot analysis of APC expression in COLO 205 whole cell lysate.

APC (C-20) - sc-896 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
APC (C-20): sc-896. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.

APC (N-15) - sc-895 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
APC (N-15): sc-895. Immunofluorescence staining of methanol-fixed NIH/3T3 mouse cells showing cytoplasmic localization.

APC (F-3) - sc-9998 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
APC (F-3): sc-9998. Western blot analysis of APC expression in Jurkat whole cell lysate.

APC (H-290) - sc-7930 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
APC (H-290): sc-7930. Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells.

APC (ALi 12-28) - sc-53165 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
APC (ALi 12-28): sc-53165. Western blot analysis of truncated APC expression COLO 320DM whole cell lysate.

APC (c-APC 28.9) - sc-53166 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
APC (c-APC 28.9): sc-53166. Western blot analysis of APC expression in HeLa whole cell lysate.

Anti-APC antibody - ab15270 from Abcam

Prices $392.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab15270 stained A431 cells. The cells were 4% paraformalehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15270, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-APC antibody - ab52223 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of APC expression in paraffin embedded human colon carcinoma tissue using ab52223. Left: untreated section. Right: section treated with immunizing peptide.

Anti-APC antibody [ALi 12-28] - ab58 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone ALi 12-28
Overlay histogram showing HCT116 cells stained with ab58 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-APC antibody [Ali 12-28] - N-terminal (FITC) - ab187800 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal

Anti-APC antibody [Ali 12-28] - N-terminal (HRP) - ab187799 from Abcam

Prices $372.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone Ali 12-28

Anti-APC antibody [CC-1] - ab16794 from Abcam

Prices $391.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone CC-1
ICC/IF image of ab16794 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16794, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-APC antibody [EMM43] - ab50801 from Abcam

Prices $378.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone EMM43
ab50801 at 1/20 dilution staining epithelial cells from human bowel; paraffin embedded.

Anti-APC antibody [EP701Y] - ab40778 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP701Y
Anti-APC antibody [EP701Y] (ab40778) at 1/1000 dilution + 10 ug/ml HEK293 cell lysateSecondary1:2000 goat anti-rabbit HRP labeled Observed band size : 160 kDa (why is the actual band size different from the predicted?)Additional bands at : 250 kDa. We are unsure as to the identity of these extra bands.

Anti-APC antibody [EP701Y] (HRP) - ab193785 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP701Y
IHC image of APC staining in a section of formalin-fixed paraffin-embedded human colon adenocarcinoma*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab193785 at 1µg/ml. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and manual) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-APC antibody - C-terminal - ab154906 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-APC antibody - C-terminal (ab154906) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg

Anti-APC (phospho S2054) antibody - ab59421 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab59421, at a 1/50 dilution, staining paraffin embedded human brain tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the phosphopeptide.

Anti-APC antibody - ab59383 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab59383 at a 1/50 dilution.Left image un-treated.Right image treated with peptide.

Anti-APC antibody, prediluted - ab15271 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
ab15271 staining APC in colon carcinoma by Immunohistochemistry (FFPE-sections).

APC/Cy5.5® Conjugation Kit - ab102855 from Abcam

Prices $225.00, $237.00, $391.00, $553.00
Sizes 100 µg, 30 µg, 300 µg, 1 mg
This illustration demonstrates a general procedure and will slightly vary dependent on the conjugate used.

APC/Cy7® Conjugation Kit - ab102859 from Abcam

Prices $225.00, $237.00, $391.00, $553.00
Sizes 100 µg, 30 µg, 300 µg, 1 mg
This illustration demonstrates a general procedure and will slightly vary dependent on the conjugate used.

Anti-APC Antibody (aa1-433, clone ALi 12-28) - LS-C2599 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-APC Antibody (aa1-433) - LS-C3765 from LifeSpan Bioscience

Prices $435.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
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