Regulation of actin cytoskeleton



PathScan ® Total β-Actin Sandwich ELISA Kit - 7880 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1. Treatment of HeLa cells with IFN-α stimulates phosphorylation of Stat3 at Tyr705 as detected by PathScan ® Phospho-Stat3 (Tyr705) Sandwich ELISA Kit #7300, but does not affect the level of β-actin as detected by PathScan ® Total β-Actin Sandwich ELISA Kit #7880. HeLa cells were treated with 100 ng/ml IFN-α for ten minutes at 37ºC before lysis. Absorbance at 450 nm is shown in the top figure, while the corresponding western blots using Phospho-Stat3 (Tyr705) (3E2) Mouse mAb #9138 (right panel) or Total β-Actin (13E5) Rabbit mAb #4970 (left panel) are shown in the bottom figure.

PathScan ® Total β-Actin Sandwich ELISA Antibody Pair - 7881 from Cell Signaling Technology

Category Antibody Pair
Prices $459.00
Sizes 1 kit (Reagents for 4 x 96 well plates)
Figure 1. The relationship between the protein concentration of the lysate from HeLa cells and the absorbance at 450 nm using the PathScan ® Total β-Actin Sandwich ELISA Antibody Pair is shown.

SimpleChIP ® Human β-Actin 3' UTR Primers - 13669 from Cell Signaling Technology

Category ChIP Kit
Prices $102.00
Sizes 500 µl (250 PCR reactions)
SimpleChIP ® Human β-Actin 3' UTR Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. Real-time PCR was performed in duplicate on a serial dilution of 2% total input DNA (20 ng, 4 ng, 0.8 ng, and 0.16 ng) using a real-time PCR detection system and SYBR ® Green reaction mix. The PCR amplification efficiency (E) and correlation coefficient (R 2 ) were calculated based on the corresponding threshold cycle (C T ) of each dilution sample during 40 cycles of real-time PCR (95°C denaturation for 15 sec, 65°C anneal/extension for 60 sec).

SimpleChIP ® Human β-Actin Promoter Primers - 13653 from Cell Signaling Technology

Category ChIP Kit
Prices $102.00
Sizes 500 µl (250 PCR reactions)
SimpleChIP ® Human β-Actin Promoter Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. Real-time PCR was performed in duplicate on a serial dilution of 2% total input DNA (20 ng, 4 ng, 0.8 ng, and 0.16 ng) using a real-time PCR detection system and SYBR ® Green reaction mix. The PCR amplification efficiency (E) and correlation coefficient (R 2 ) were calculated based on the corresponding threshold cycle (C T ) of each dilution sample during 40 cycles of real-time PCR (95°C denaturation for 15 sec, 65°C anneal/extension for 60 sec).

Human ACTB / Beta Actin ELISA Kit (Sandwich ELISA) - LS-F10737 from LifeSpan Bioscience

Category ELISA Kit
Prices $600.00
Sizes 1 plate
Sensitivity 0.312 ng/ml
Range 0.312-20 ng/ml

Beta-actin, Human, ELISA Kit - CSB-E13298h from ARP American Research Products

Category ELISA Kit
Prices $495.00, $695.00, $696.00, $699.00
Sizes 48 t, 96 t, 5 x 96t, 10 x 96t
Sensitivity 0.078 ng/ml
Range 0.312 ng/ml-20 ng/ml

Actin Beta, ELISA Kit - MBS729157 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Actin Beta, ELISA Kit - MBS742517 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Actin Beta, ELISA Kit - MBS736645 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Actin Beta, ELISA Kit - MBS724618 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Actin Beta, ELISA Kit - MBS735129 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
Sensitivity 0.1 ng/mL.

Actin Beta, ELISA Kit - MBS740507 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Actin Beta, ELISA Kit - MBS734373 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
Sensitivity 1.0 ng/mL.

Actin beta, ELISA Kit - MBS726697 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
Sensitivity 0.1 ng/mL.

Actin Beta, ELISA Kit - MBS742842 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Actin Beta, ELISA Kit - MBS721123 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
Sensitivity 0.1 ng/mL.

Actin Beta, ELISA Kit - MBS736752 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Actin Beta, ELISA Kit - MBS728822 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

actin, beta, ELISA Kit - MBS702655 from MyBioSource

Category ELISA Kit
Prices $250.00, $495.00, $710.00, $2,705.00, $5,145.00
Sizes 24 -strip-wells (Limit 1), 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
Sensitivity <0.078 ng/ml
Range 0.312 ng/ml-20 ng/ml

ELISA Kit for Actin Beta (ACTb) - SEB340Mi from Uscn Life Science Inc.

Category ELISA Kit
Sensitivity The minimum detectable dose of this kit is typically less than 0.64ng/mL.
Range 1.56-100ng/mL The standard curve concentrations used for the ELISA’s were 100ng/mL, 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.13ng/mL, 1.56ng/mL

CLIA Kit for Actin Beta (ACTb) - SCB340Mi from Uscn Life Science Inc.

Category CLIA Kit
Sensitivity The minimum detectable dose of this kit is typically less than 0.05ng/mL.
Range 0.14-100ng/mL The standard curve concentrations used for the CLIA’s were 100ng/mL, 33.33ng/mL, 11.11ng/mL, 3.7ng/mL, 1.23ng/mL, 0.41ng/mL, 0.14ng/mL

Human β-actin ELISA Kit - CSB-E13298h from Cusabio

Category ELISA Kit
Sensitivity 0.078 ng/ml
Range 0.312 ng/ml-20 ng/ml

ELISA Kit for Actin Beta (ACTb) - SEB340Mi from Cloud-clone

Category ELISA Kit
Sensitivity The minimum detectable dose of this kit is typically less than 0.64ng/mL.
Range 1.563-100ng/mL The standard curve concentrations used for the ELISA's were 100ng/mL, 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.125ng/mL, 1.563ng/mL

CLIA Kit for Actin Beta (ACTb) - SCB340Mi from Cloud-clone

Category CLIA Kit
Sensitivity The minimum detectable dose of this kit is typically less than 0.05ng/mL.
Range 0.137-100ng/mL The standard curve concentrations used for the ELISA's were 100ng/mL, 33.33ng/mL, 11.11ng/mL, 3.7ng/mL, 1.235ng/mL, 0.412ng/mL, 0.137ng/mL

Rat ACTB / Beta Actin ELISA Kit (Custom ELISA) - LS-F20160 from LifeSpan Bioscience

Category ELISA Kit
Prices $600.00
Sizes 1 plate
Sensitivity To be determined
Range 0.312-20 ng/ml
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