PRKCG

Gene Symbol PRKCG
Entrez Gene 5582
Alt Symbol PKC-gamma, PKCC, PKCG, SCA14
Species Human
Gene Type protein-coding
Description protein kinase C, gamma
Other Description protein kinase C gamma type
Swissprots B7Z8Q0 P05129
Accessions CAA44394 EAW72161 P05129 AB209671 BAD92908 AF345987 AAK13533 AK296412 BAH12345 AK296434 BAH12352 AK302958 BAH13856 AK303741 BAH14036 AK312302 BAG35228 BC047876 AAH47876 BI757265 M13977 AAA60102 Z15114 CAA78820 XM_011527107 XP_011525409 XM_011527108 XP_011525410 NM_002739 NP_002730
Function Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GRIA4/GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor GRM5/MGLUR5 and phosphorylates GRIN1/NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but ma
Subcellular Location Cytoplasm {ECO:0000250|UniProtKB:P63318}. Cytoplasm, perinuclear region {ECO:0000250}. Cell membrane {ECO:0000250|UniProtKB:P63318}; Peripheral membrane protein {ECO:0000250}. Cell junction, synapse, synaptosome {ECO:0000250|UniProtKB:P63318}. Cell projection, dendrite {ECO:0000250|UniProtKB:P63319}. Note=Translocates to synaptic membranes on stimulation. {ECO:0000250|UniProtKB:P63318}.
Tissue Specificity Expressed in Purkinje cells of the cerebellar cortex. {ECO:0000269|PubMed:12644968}.
Top Pathways Thyroid hormone synthesis, Proteoglycans in cancer, Amphetamine addiction, Sphingolipid signaling pathway, Morphine addiction

Phospho-PKCζ/λ (Thr410/403) Antibody - 9378 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 cells, untreated or EGF-treated (100 ng/ml) and purified PKCzeta, untreated or lambda phosphatase-treated, using Phospho-PKCzeta/lambda (Thr410/403) Antibody (upper) or PKCzeta antibody (lower).

PKC γ (C-19) - sc-211 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PKC γ (C-19): sc-211. Western blot analysis of PKC γ expression in 3611-RF transformed rat fibroblasts.

PKC γ (C-4) - sc-166385 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PKC γ (C-4): sc-166385. Western blot analysis of PKC γ expression in non-transfected: sc-117752 (A) and human PKC γ transfected: sc-116200 (B) 293T whole cell lysates.

PKC γ (D-4) - sc-166424 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PKC γ (D-4): sc-166424. Western blot analysis of PKC γ expression in non-transfected: sc-117752 (A) and human PKC γ transfected: sc-116200 (B) 293T whole cell lysates.

PKC γ (D-4) - sc-166451 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PKC γ (D-4): sc-166451. Western blot analysis of PKC γ expression in non-transfected: sc-117752 (A) and human PKC γ transfected: sc-116200 (B) 293T whole cell lysates.

PKC γ (N-16) - sc-30793 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PKC γ (N-16): sc-30793. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

Anti-PKC gamma (phospho T514) antibody [EP2730Y] - ab109539 from Abcam

Prices $99.00, $387.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2730Y
All lanes : Anti-PKC gamma (phospho T514) antibody [EP2730Y] (ab109539) at 1/20000 dilutionLane 1 : HeLa cell lysate, untreatedLane 2 : HeLa cell lysate treated with LPLysates/proteins at 10 µg per lane.SecondaryHRP-labelled goat anti-rabbit at 1/2000 dilution

Anti-PKC gamma (phospho T655) antibody - ab5796 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Peptide Competition and Phosphatase treatment: Lysates prepared from HeLa cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-11) or treated with lambda (ë) phosphatase (12), blocked with a 3% low-fat milk-TBST buffer overnight at 4°C, and incubated with 0.50 µg/mL ab5796 antibody for two hours at room temperature in a 3% BSA TBST buffer, following prior incubation with: no peptide (1, 11, 12), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine containing peptide (3), the phosphopeptide immunogen (4), or, the phosphopeptide corresponding to the immunogen from other PKC isoforms (5-10). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStarTM method. The data show that the peptide corresponding to PKC gamma [pT655] blocks the antibody signal while the peptides corresp

Anti-PKC gamma (phospho T674) antibody - ab5797 from Abcam

Prices $380.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Peptide Competition and Phosphatase Treatment: Lysates prepared from HeLa cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-13) or treated with lambda (ë) phosphatase (14), blocked with a 5% BSA-TBST buffer overnight at 4°C, and incubated with 0.50 µg/mL ab5797 antibody for two hours at room temperature in a 3% BSA TBST buffer, following prior incubation with: the phosphopeptide corresponding to the immunogen from other PKC isoforms (1-9), the phosphopeptide immunogen (10), the non-phosphopeptide corresponding to the immunogen (11), a generic phosphothreonine containing peptide (12) or, no peptide (13, 14). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP-conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that the peptide corresponding to PKC gamma [pT674] blocks the antibody signal. The peptides corresponding to PKC alpha [pT65

Anti-PKC gamma antibody - ab71558 from Abcam

Prices $390.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC gamma antibody (ab71558) at 1/2000 dilutionLane 1 : Hela cell lysateLane 2 : A431 cell lysateLane 3 : Mouse brain lysateLane 4 : Rat brain lysate

Anti-PKC gamma antibody - ab4144 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
See Abreview

Anti-PKC gamma antibody [EPR2750] - ab108961 from Abcam

Prices $99.00, $379.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2750
All lanes : Anti-PKC gamma antibody [EPR2750] (ab108961) at 1/1000 dilutionLane 1 : Human fetal brain cell lysateLane 2 : Human fetal cerebellum cell lysateLysates/proteins at 10 µg per lane.

Anti-PKC gamma antibody [EPR5979(2)] - ab131222 from Abcam

Prices $99.00, $378.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5979(2)
Anti-PKC gamma antibody [EPR5979(2)] (ab131222) at 1/1000 dilution + Human midbrain lysate at 10 µgSecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-PRKCG / PKC-Gamma Antibody (clone PKC66) - LS-C17456 from LifeSpan Bioscience

Prices $455.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal
Clone PKC66

Anti-PRKCG / PKC-Gamma Antibody (C-Terminus) - LS-C17457 from LifeSpan Bioscience

Prices $445.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal

Anti-PRKCG / PKC-Gamma Antibody (C-Terminus) - LS-C17459 from LifeSpan Bioscience

Prices $335.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal

Anti-PRKCG / PKC-Gamma Antibody (aa684-697) - LS-C17458 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PRKCG / PKC-Gamma Antibody (phospho-Thr514) - LS-C17462 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PRKCG / PKC-Gamma Antibody (phospho-Thr655) - LS-C17463 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PRKCG / PKC-Gamma Antibody (C-Terminus) - LS-C38546 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PRKCG / PKC-Gamma Antibody - LS-C91498 from LifeSpan Bioscience

Prices $295.00
Sizes 40 µg
Host Rabbit
Clonality Polyclonal

Anti-PRKCG / PKC-Gamma Antibody - LS-C91497 from LifeSpan Bioscience

Prices $295.00
Sizes 40 µg
Host Rabbit
Clonality Polyclonal

Anti-PRKCG / PKC-Gamma Antibody (clone K21-L, FITC) - LS-C170830 from LifeSpan Bioscience

Prices $495.00
Sizes 100 tst
Host Rabbit
Clonality Polyclonal
Clone K21-L

Anti-PRKCG / PKC-Gamma Antibody (aa71-120) - LS-C111917 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PRKCG / PKC-Gamma antibody LS-C111917 Western blot of Placenta lysate.

Anti-PRKCG / PKC-Gamma Antibody (aa305-336) - LS-C98926 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human brain tissue reacted with PKC gamma antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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