HSPB1

Gene Symbol HSPB1
Entrez Gene 3315
Alt Symbol CMT2F, HEL-S-102, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27
Species Human
Gene Type protein-coding
Description heat shock 27kDa protein 1
Other Description 28 kDa heat shock protein|HSP 27|epididymis secretory protein Li 102|estrogen-regulated 24 kDa protein|heat shock 27 kDa protein|heat shock 27kD protein 1|heat shock protein beta-1|stress-responsive protein 27
Swissprots Q6FI47 Q96EI7 Q9UC35 Q96C20 B2R4N8 Q9UC36 Q9UC31 P04792 Q9UC34
Accessions AAA62175 ABC88475 CDM22284 EAW71803 P04792 AB020027 BAB17232 AF086135 AK296890 BAG59449 AK311446 AK311894 BAG34835 AL050380 BC000510 AAH00510 BC012292 AAH12292 BC012768 AAH12768 BC014920 AAH14920 BC073768 AAH73768 BP231480 BT019888 AAV38691 CR407614 CAG28542 CR536489 CAG38728 DQ891304 ABM82230 DQ893349 ABM84275 DQ894489 ABM85415 DQ896669 ABM87668 FJ224323 ACI46015 S74571 AAB20722 U90906 AAB51056 X16477 CAA34498 X54079 CAA38016 NM_001540 NP_001531
Function Involved in stress resistance and actin organization.
Subcellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
Tissue Specificity Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle. {ECO:0000269|PubMed:1560006}.
Top Pathways VEGF signaling pathway, MAPK signaling pathway, Amoebiasis, Epstein-Barr virus infection

Phospho-HSP27 (Ser82) (D1H2F6) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 12172 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D1H2F6
Confocal immunofluorescent analysis of HeLa cells, untreated (top left) or UV-treated (top right), C2C12 cells, untreated (bottom left) or treated with λ phosphatase (bottom middle), and NIH/3T3 cells (bottom right) using Phospho-HSP27 (Ser82) (D1H2F6) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Negative staining in NIH/3T3 cells is in agreement with the observation that NIH/3T3 cells do not express HSP27 under basal conditions (5,7).

Phospho-HSP27 (Ser82) (D1H2F6) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 11892 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D1H2F6
Flow cytometric analysis of HeLa cells, untreated (blue) or UV-treated (green), using Phospho-HSP27 (Ser82) (D1H2F6) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate).

Phospho-HSP27 (Ser82) (D1H2F6) XP ® Rabbit mAb - 9709 from Cell Signaling Technology

Prices $119.00, $318.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1H2F6
Western blot analysis of extracts from HeLa or HT-29 cells, untreated (-) or treated (+) with either UV (40 mJ/cm 2 with 30 min recovery) or anisomycin (25 μg/mL, 30 min), using Phospho-HSP27 (Ser82) (D1H2F6) XP ® Rabbit mAb.

Phospho-HSP27 (Ser15) Antibody - 2404 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa and COS cells, untreated, anisomycin-treated or UV-treated, using Phospho-HSP27 (Ser15) Antibody (upper) or HSP27 (G31) Monoclonal Antibody #2402 (lower).

Phospho-HSP27 (Ser78) Antibody - 2405 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa and COS cells, untreated, anisomycin-treated or UV-treated, using Phospho-HSP27 (Ser78) Antibody (upper) or HSP27 (G31) mAb #2402 (lower).

Phospho-HSP27 (Ser82) Antibody II - 2406 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa and PC12 cells, treated with λ-phosphatase or UV as indicated, using Phospho-HSP27 (Ser82) Antibody II.

Phospho-HSP27 (Ser82) Antibody - 2401 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells, using Phospho-HSP27 (Ser82) Antibody (upper) or control HSP27 antibody (lower). HeLa cells were incubated at 42ºC for 0-2 hours as indicated.

HSP27 (G31) Mouse mAb - 2402 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone G31
Western blot analysis of extracts from HeLa and COS-7 cells using HSP27 (G31) Mouse mAb.

HSP 27 (C-20) - sc-1048 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HSP 27 (C-20): sc-1048. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human breast tissue showing intense cytoplasmic staining of selected cells.

HSP 27 (F-4) - sc-13132 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HSP 27 (F-4): sc-13132. Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing cytoplasmic staining in surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

HSP 27 (H-77) - sc-9012 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HSP 27 (H-77): sc-9012. Western blot analysis of HSP 27 expression in HeLa whole cell lysate.

HSP 27 (M-20) - sc-1049 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HSP 27 (M-20): sc-1049. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing cytoplasmic staining of mucosal epithelia.

HSP 27 (HSP25-31) - sc-51958 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
HSP 27 (HSP25-31): sc-51958. Western blot analysis of HSP 27 expression in HeLa (A) and heat shock treated HeLa (B) whole cell lysates.

HSP 27 (HSP25-19) - sc-51956 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
HSP 27 (HSP25-19): sc-51956. Western blot analysis of HSP 27 expression in HeLa (A) and heat shock treated HeLa (B) whole cell lysates.

HSP 27 (G3.1) - sc-59562 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
HSP 27 (G3.1): sc-59562. Western blot analysis of HSP 27 expression in HeLa (A) and heat shock treated HeLa (B) whole cell lysates.

p-HSP 27 (B-3) - sc-166693 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of HSP 27 phosphorylation in untreated (A,D), UV irradiated (B,E) and UV irradiated and lambda protein phosphatase treated (C,F) HeLa whole cell lysates. Antibodies tested include p-HSP 27 (B-3): sc-166693 (A,B,C) and HSP 27 (F-4): sc-13132 (D,E,F).

p-HSP 27 (D-3) - sc-166694 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of HSP 27 phosphorylation in untreated (A,D), UV irradiated (B,E) and UV irradiated and lambda protein phosphatase treated (C,F) HeLa whole cell lysates. Antibodies tested include p-HSP 27 (D-3): sc-166694 (A,B,C) and HSP 27 (F-4): sc-13132 (D,E,F).

p-HSP 27 (Ser 15) - sc-12359 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-HSP 27 (Ser 15)-R: sc-12359-R. Western blot analysis of HSP 27 phosphorylation in non-transfected: sc-117752 (A) and human HSP 27 transfected: sc-174710 (B) 293T whole cell lysates.

p-HSP 27 (Ser 78)-R - sc-16568-R from Santa Cruz Biotechnology

Prices $61.00
Sizes 100 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
p-HSP 27 (Ser 78)-R: sc-16568-R. Immunoperoxidase staining of formalin fixed, paraffin-embedded human vulva/anal skin tissue showing cytoplasmic and nuclear staining of squamous epithelial cells.

p-HSP 27 (Ser 82) - sc-12923 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-HSP 27 (Ser 82)-R: sc-12923-R. Western blot analysis of HSP 27 phosphorylation in non-transfected: sc-117752 (A) and human HSP 27 transfected: sc-174710 (B) 293T whole cell lysates.

p-HSP 27 (Ser 82) - sc-101700 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-HSP 27 (Ser 82): sc-101700. Western blot analysis of phosphorylated HSP 27 expression in untreated (A), UV-treated (B) and Heat Shock-treated (C) HeLa whole cell lysates.

p-HSP 27 (Ser 15) - sc-101699 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-HSP 27 (Ser 15): sc-101699. Western blot analysis of phosphorylated HSP 27 expression in untreated (A) and UV-treated (B) HeLa whole cell lysates.

p-HSP 27 (5B9) - sc-81498 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
p-HSP 27 (5B9): sc-81498. Western blot analysis of HSP 27 phosphorylation in non-transfected 293T: sc-117752 (A), mouse HSP 27 transfected 293T: sc-120910 (B) and ECV304 (C) whole cell lysates.

Anti-Hsp25 (phospho S78 + S82) antibody - ab138366 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling Hsp25 with ab138366 at 1/50 dilution.

Anti-Hsp25 (phospho S78) antibody - ab194773 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Hsp25 (phospho S78) antibody (ab194773) at 1/500 dilutionLane 1 : HL60 cell extract, untreatedLane 2 : HL60 cell extract, treated with UV
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