DIABLO

Gene Symbol DIABLO
Entrez Gene 56616
Alt Symbol DFNA64, SMAC
Species Human
Gene Type protein-coding
Description diablo, IAP-binding mitochondrial protein
Other Description diablo homolog, mitochondrial|direct IAP-binding protein with low pI|second mitochondria-derived activator of caspase
Swissprots Q9HAV6 Q9BT11 B2RDQ0 Q6W3F3 Q96LV0 Q9NR28
Accessions EAW98311 EAW98312 EAW98313 EAW98314 EAW98315 EAW98316 Q9NR28 AF262240 AAF87716 AF298770 AAG22077 AK001399 BAG50903 AK024768 BAB14994 AK057778 BAB71568 AK222664 BAD96384 AK315629 BAG37997 AY313210 AAQ86939 BC004417 AAH04417 BC011909 AAH11909 BC046209 BC095468 AAH95468 BF313712 BF724951 BM746689 CD742753 CF128595 DA877400 DA910387 DA915183 DB254800 DQ893061 ABM83987 DQ896313 ABM87312 JX679472 AFX61342 N66735 NM_001278302 NP_001265231 NM_001278303 NP_001265232 NM_001278304 NP_001265233 NM_001278342 NP_001265271 NM_019887 NP_063940 NM_138930 NP_620308 NM_138929
Function Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. {ECO:0000269|PubMed:10929711, ECO:0000269|PubMed:14523016, ECO:0000269|PubMed:15200957}.
Subcellular Location Mitochondrion {ECO:0000269|PubMed:14523016}. Note=Released into the cytosol when cells undergo apoptosis.
Tissue Specificity Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed. {ECO:0000269|PubMed:10929711, ECO:0000269|PubMed:14523016}.

Smac/Diablo Mouse mAb - 2954 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Western blot analysis of extracts from 293, HeLa and Jurkat cells, using Smac/Diablo Mouse mAb.

Smac/Diablo Antibody - 2952 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Smac (C-10) - sc-393118 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Smac (C-10): sc-393118. Western blot analysis of Smac expression in Hep G2 (A), HeLa (B), Caki-1 (C), DU 145 (D) and KNRK (E) whole cell lysates. Note lack of reactivity in lane E.

Smac (C-20) - sc-12684 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Smac (C-20): sc-12684. Western blot analysis of Smac expression in HeLa (A), Caki-1 (B), DU 145 (C), Hep G2 (D) and KNRK (E) whole cell lysates.

Smac (H-177) - sc-22766 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Smac (H-177): sc-22766. Immunofluorescence staining of normal mouse lymph node frozen section showing membrane staining.

Smac (V-17) - sc-12683 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Smac siRNA (h): sc-36505. Immunofluorescence staining of methanol-fixed, control HeLa (A) and Smac siRNA silenced HeLa (B) cells showing diminished cytoplasmic staining in the siRNA silenced cells. Cells probed with Smac (V-17): sc-12683.

Smac (56) - sc-136302 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Smac (56): sc-136302. Western blot analysis of Smac expression in HeLa whole cell lysate.

Anti-Smac / Diablo antibody - ab8115 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Smac / Diablo antibody (ab8115) at 1 µg/mlLane 1 : Mouse heart tissue lysate with absence of blocking peptideLane 2 : Mouse heart tissue lysate with presence of blocking peptideLane 3 : Rat heart tissue lysate with absence of blocking peptideObserved band size : 25 kDa (why is the actual band size different from the predicted?)

Anti-Smac / Diablo antibody - ab8114 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Smac / Diablo antibody (ab8114) at 1 µg/mlLane 1 : human heart tissue lysate with absence of blocking peptideLane 2 : human heart tissue lysate with Smac / Diablo peptide (ab8380)

Anti-Smac / Diablo antibody - ab68352 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
All lanes : Anti-Smac / Diablo antibody (ab68352) at 1 µg/mlLane 1 : Smac / Diablo transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-Smac / Diablo antibody - ab66290 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Smac / Diablo antibody (ab66290) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLane 8 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-Smac / Diablo antibody - ab10919 from Abcam

Prices $359.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab10919, at a dilution of 5 µg /ml, staining Smac/Diablo in human ovary tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-Smac / Diablo antibody - ab23450 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Smac / Diablo antibody (ab23450) at 1 µg/ml + HepG2 lysateSecondaryHRP conjugated anti-Rabbit IgG (1: 50,000-100,000)

Anti-Smac / Diablo antibody - ab137462 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Smac / Diablo antibody (ab137462) at 1/1000 dilution + A431 whole cell lysate at 30 µg

Anti-Smac / Diablo antibody - ab2448 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-Smac / Diablo antibody - ab13817 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Smac / Diablo antibody [2D12] - ab25170 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2D12

Anti-Smac / Diablo antibody [79-1-83] - ab69509 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 79-1-83
All lanes : Anti-Smac / Diablo antibody [79-1-83] (ab69509) at 1 µg/mlLane 1 : MW markerLane 2 : 293 cell lysateLane 3 : Hela cell lysateLane 4 : Jurkat cell lysateLane 5 : Vero cell lysatedeveloped using the ECL technique

Anti-Smac / Diablo antibody [8H5AA3] - ab110288 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 8H5AA3
HeLa cells were stained with 1 µg/mL Smac / Diablo antibody (ab110288) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

Anti-Smac / Diablo antibody [8H5AB2] - ab110291 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 8H5AB2
All lanes : Anti-Smac / Diablo antibody [8H5AB2] (ab110291) at 1 µg/mlLane 1 : Human liver mitichondria Lane 2 : HepG2 cells

Anti-Smac / Diablo antibody [AT19F2] - ab177526 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone AT19F2
Anti-Smac / Diablo antibody [AT19F2] (ab177526) at 1/3000 dilution + Jurkat cell line lysate at 40 µgSecondaryGoat anti-mouse HRP conjugated antibodydeveloped using the ECL technique

Anti-Smac / Diablo antibody [EPR10934] - ab174284 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10934
Immunohistochemical analysis of paraffinn embedded Human testis tissue labeling Smac / Diablo with ab174284 at 1/50.

Anti-Smac / Diablo antibody [SMAC 17 1-87] - ab111893 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone SMAC 17 1-87
ab111893 labelling Smac / Diablo (green) in the cytoplasm of A431 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse IgG was used as the secondary antibody. Red (phalloidiin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

Anti-Smac / Diablo antibody [Y12] - ab32023 from Abcam

Prices $400.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y12
ab32023, at a 1/10 dilution, staining human Smac/Diablo in skin cancer tissue by Immunohistochemistry, Paraffin embedded tissue

Anti-Smac / Diablo antibody [Y12] (Alexa Fluor® 488) - ab199317 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y12
ab199317 staining Smac / Diablo in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199317 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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