FOXA2

Gene Symbol FOXA2
Entrez Gene 3170
Alt Symbol HNF3B, TCF3B
Species Human
Gene Type protein-coding
Description forkhead box A2
Other Description HNF-3-beta|HNF-3B|TCF-3B|forkhead box protein A2|hepatic nuclear factor-3-beta|hepatocyte nuclear factor 3, beta|hepatocyte nuclear factor 3-beta|transcription factor 3B
Swissprots Q8WUW4 Q96DF7 Q9Y261
Accessions AAD41081 AAD51978 ACA06111 CAJ01277 CAJ01278 EAX10174 EAX10175 Q9Y261 AA469087 AB028021 BAA78106 AI268650 AI302734 AK301611 BAG63097 BC006545 AAH06545 BC011780 AAH11780 BC019288 AAH19288 BM273279 BQ643619 DQ892883 ABM83809 DQ896132 ABM87131 XM_006723562 XP_006723625 XM_011529231 XP_011527533 NM_021784 NP_068556 NM_153675 NP_710141
Function Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes im
Subcellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00089, ECO:0000269|PubMed:14500912}. Cytoplasm {ECO:0000269|PubMed:14500912}. Note=Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner; in response to insulin signaling via AKT1 is exported from the nucleus.
Top Pathways Maturity onset diabetes of the young

FoxA2/HNF3β (D56D6) XP ® Rabbit mAb - 8186 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D56D6
Western blot analysis of extracts from Hep G2 cells using FoxA2/HNF3β (D56D6) XP ® Rabbit mAb.

FoxA2/HNF3β Antibody - 3143 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HepG2 cells using FoxA2/HNF3β Antibody.

HNF-3β (H-150) - sc-20692 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HNF-3β (H-150): sc-20692. Western blot analysis of HNF-3β expression in non-transfected: sc-117752 (A) and human HNF-3β transfected: sc-176240 (B) 293T whole cell lysates.

HNF-3β (M-20) - sc-6554 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HNF-3β (M-20): sc-6554. Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast cancer showing nuclear staining of tumor cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

HNF-3β (P-19) - sc-9187 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HNF-3β (P-19): sc-9187. Western blot analysis of HNF-3β expression in non-transfected: sc-117752 (A) and human HNF-3β transfected: sc-176240 (B) 293T whole cell lysates.

HNF-3β (RY-7) - sc-101060 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
HNF-3β (RY-7): sc-101060. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human stomach tissue showing nuclear localization.

Anti-FOXA2 antibody - ab60721 from Abcam

Prices $390.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-FOXA2 antibody (ab60721) at 1 µg/ml + HepG2 cell lysate at 25 µg

Anti-FOXA2 antibody - ab5074 from Abcam

Prices $382.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-FOXA2 antibody (ab5074) at 1 µg/ml + Human Liver lysate at 1 µg/ml

Anti-FOXA2 antibody - ab23630 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FOXA2 antibody (ab23630) at 1 µg/mlLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate (ab14660)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-FOXA2 antibody - ab83517 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-FOXA2 antibody (ab83517) at 1/2000 dilution + Huh 7 cell lysate

Anti-FOXA2 antibody - ab82827 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FOXA2 antibody (ab82827) at 1/1000 dilutionLane 1 : Uninduced culture of E.coli (one shot Top10 competent cells)Lane 2 : Induced culture of E.coli (one shot Top10 competent cells)SecondaryAnti-Rabbit IgG HRP at 1/1000 dilution

Anti-FOXA2 antibody - ab191577 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-FOXA2 antibody (ab191577) at 1 µg/ml + Human bladder tissue lysate at 15 µg

Anti-FOXA2 antibody [1C7] - ab117542 from Abcam

Prices $385.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 1C7
Human Breast, Formalin-Fixed, Paraffin-Embedded tissue, stained with ab117542 at 5µg/ml.

Anti-FOXA2 antibody [3C10] - ab89997 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3C10
All lanes : Anti-FOXA2 antibody [3C10] (ab89997) at 1/1000 dilutionLane 1 : control plasmid-transfected HEK293 cell lysateLane 2 : FOXA2-transfected HEK293 cell lysateLysates/proteins at 5 µg per lane.

Anti-FOXA2 antibody [7H4B7] - ab130188 from Abcam

Host Mouse
Clonality Monoclonal
Clone 7H4B7
Anti-FOXA2 antibody [7H4B7] (ab130188) at 1/500 dilution + A549 cell lysate

Anti-FOXA2 antibody [EPR4465] - ab108396 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4465
Immunofluorescence staining of HepG2 cells with purified ab108396 at a working dilution of 1 in 300, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab108396 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.

Anti-FOXA2 antibody [EPR4466] - ab108422 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4466
Anti-FOXA2 antibody [EPR4466] (ab108422) at 1/10000 dilution (purified) + Human colon cancer tissue lysate at 20 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Anti-FOXA2 antibody [EPR4466] (Alexa Fluor® 488) - ab193864 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4466
ab193864 staining FOXA2 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab193864 at a working dilution of 1 in 50 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-FOXA2 antibody [EPR4466] (Alexa Fluor® 647) - ab193879 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4466
ab193879 staining FOXA2 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab193879 at a working dilution of 1 in 50 (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-FOXA2 antibody [EPR4466] (HRP) - ab193880 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4466
Anti-FOXA2 antibody [EPR4466] (HRP) (ab193880) at 1/1000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µgdeveloped using the ECL techniquePerformed under reducing conditions.

Anti-FOXA2 antibody [PR-3G1] - ab95327 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone PR-3G1
All lanes : Anti-FOXA2 antibody [PR-3G1] (ab95327) at 1 µg/mlLane 1 : HEK-293-FOXA2 transfectant cell lysate at 7.5 µgLane 2 : Untransfected HEK-293 cell lysate at 7.5 µgLane 3 : FOXA2 recombinant protein at 0.25 µg

Anti-FOXA2 Antibody (Internal) - LS-C201056 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of FOXA2 in human bladder tissue with FOXA2 antibody at 20 ug/ml.

Anti-FOXA2 Antibody (aa355-389) - LS-C344527 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of lysates from HepG2, HT-29, SW620 cell line (from left to right) with FOXA2 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.

Anti-FOXA2 Antibody (aa288-337) - LS-C109625 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
FOXA2 antibody LS-C109625 Western blot of HeLa lysate.

Anti-FOXA2 Antibody (aa271-320) - LS-C116045 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
FOXA2 antibody LS-C116045 Western blot of Mouse Thymus lysate.
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