NOX4

Gene Symbol NOX4
Entrez Gene 50507
Alt Symbol KOX, KOX-1, RENOX
Species Human
Gene Type protein-coding
Description NADPH oxidase 4
Other Description kidney oxidase-1|kidney superoxide-producing NADPH oxidase|renal NAD(P)H-oxidase
Swissprots Q7Z7G3 Q5K3R6 B7Z520 Q5K3R5 Q9NPH5 Q86V92 Q5K3R8 Q5K3R4 A8K715 E7EMD7
Accessions EAW59353 EAW59354 EAW59355 Q9NPH5 AB041035 BAA95695 AF254621 AAF68973 AF261943 AAF87572 AJ704725 CAG28807 AJ704726 CAG28808 AJ704727 CAG28809 AJ704728 CAG28810 AJ704729 CAG28811 AK057189 BAG51879 AK291830 BAF84519 AK298323 BAH12756 AK298328 BAH12759 AK298357 BAH12765 AK298376 BAH12771 AK311381 AK311389 AW207163 AY288918 AAP41109 BC040105 AAH40105 BC051371 AAH51371 BP376400 BU730205 DA970477 JF432404 ADZ15621 XM_006718849 XP_006718912 XM_011542857 XP_011541159 XM_011542858 XP_011541160 XM_011542859 XP_011541161 NM_001143836 NP_001137308 NM_001143837 NP_001137309 NM_001291926 NP_001278855 NM_001291927 NP_001278856 NM_001291929 NP_001278858 NM_001300995 NP_001287924 NM_016931 NP_058627 NR_120406
Function Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity.
Subcellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. Cell junction, focal adhesion {ECO:0000305}. Note=May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus.
Tissue Specificity Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells. {ECO:0000269|PubMed:11032835, ECO:0000269|PubMed:11376945, ECO:0000269|PubMed:15210697, ECO:0000269|PubMed:16324151}.

Nox4 (H-300) - sc-30141 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Nox4 (H-300): sc-30141. Western blot analysis of Nox4 expression in mouse placenta tissue extract.

Nox4 (L-20) - sc-55142 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Nox4 (L-20): sc-55142. Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid gland tissue showing cytoplasmic and nuclear staining of glandular cells.

Nox4 (N-15) - sc-21860 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Nox4 (N-15): sc-21860. Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal tissue showing cytoplasmic staining of myocytes.

Anti-NADPH oxidase 4 antibody - ab116502 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-NADPH oxidase 4 antibody (ab116502) at 1/100 dilution + CEM cell line lysate at 35 µg

Anti-NADPH oxidase 4 antibody - ab55832 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-NADPH oxidase 4 antibody [UOTR1B492] - ab109225 from Abcam

Host Rabbit
Clonality Monoclonal
Clone UOTR1B492
Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab109225) at 1/10000 dilution (purified) + JAR cell lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-NADPH oxidase 4 antibody [UOTR1B492] (Alexa Fluor® 488) - ab195300 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone UOTR1B492
Overlay histogram showing HeLa cells stained with ab195300 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195300, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in HeLa fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-NADPH oxidase 4 antibody [UOTR1B492] (Alexa Fluor® 647) - ab195301 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone UOTR1B492
ab195301 staining NADPH oxidase 4 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab195301 at 1/35 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.This product also gave a positive signal in 4% formaldehyde (10 min) fixed HeLa cells under the same testing conditions.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-NADPH oxidase 4 antibody [UOTR1B493] - ab133303 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone UOTR1B493
All lanes : Anti-NADPH oxidase 4 antibody [UOTR1B493] (ab133303) at 1/1000 dilutionLane 1 : Fetal kidney cell lysatesLane 2 : U87-MG cell lysatesLane 3 : 293 cell lysatesLane 4 : JAR cell lysatesLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-NOX4 Antibody (aa100-200, Biotin) - LS-C83783 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NOX4 Antibody (C-Terminus) - LS-C46943 from LifeSpan Bioscience

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-NOX4 Antibody (aa100-200, HRP) - LS-C83784 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NOX4 Antibody (aa500-578, Biotin) - LS-C83785 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-NOX4 Antibody (aa100-200, DY650) - LS-C141598 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NOX4 Antibody (aa100-200, DY488) - LS-C141599 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NOX4 Antibody (aa100-200, DY550) - LS-C141600 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NOX4 Antibody (C-Terminus, DY650) - LS-C141601 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-NOX4 Antibody (C-Terminus, DY488) - LS-C141602 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-NOX4 Antibody (C-Terminus, DY550) - LS-C141603 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-NOX4 Antibody (Biotin) - LS-C181943 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-NOX4 Antibody (DY650) - LS-C181944 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-NOX4 Antibody (DY488) - LS-C181945 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-NOX4 Antibody (HRP) - LS-C181946 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-NOX4 Antibody (DY550) - LS-C181947 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-NOX4 Antibody (aa124-153, AP) - LS-C264578 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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