EIF4E

Gene Symbol EIF4E
Entrez Gene 1977
Alt Symbol AUTS19, CBP, EIF4E1, EIF4EL1, EIF4F
Species Human
Gene Type protein-coding
Description eukaryotic translation initiation factor 4E
Other Description eIF-4E|eIF-4F 25 kDa subunit|eukaryotic translation initiation factor 4E-like 1|mRNA cap-binding protein
Swissprots B7Z6V1 Q96E95 P06730 D6RCQ6
Accessions AAB07114 CAI79364 CBL95069 CBY65608 CBY65610 EAX06077 EAX06078 EAX06079 EAX06080 EAX06081 P06730 AJ844638 AK295083 BAH11967 AK300982 BAH13387 AV708571 BC012611 AAH12611 BC035166 AAH35166 BC043226 AAH43226 BC107715 AAI07716 BM849222 CA419184 DC388666 DQ890728 ABM81654 DQ893903 ABM84829 KJ534830 AHW56470 M15353 AAC13647 XM_006714126 XP_006714189 XM_006714127 XP_006714190 NM_001130678 NP_001124150 NM_001130679 NP_001124151 NM_001968 NP_001959
Function Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. {ECO:0000269|PubMed:16271312, ECO:0000269|PubMed:22578813}.
Subcellular Location Cytoplasm, P-body {ECO:0000269|PubMed:20616046}. Cytoplasm {ECO:0000250}.
Top Pathways Insulin signaling pathway, RNA transport, PI3K-Akt signaling pathway, mTOR signaling pathway

PathScan ® Phospho-eIF4E (Ser209) Sandwich ELISA Kit - 7938 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1. Treatment of serum-starved HeLa cells with 20% FBS stimulates phosphorylation of eIF4E at Ser209, detected by the PathScan ® Phospho-eIF4E (Ser209) Sandwich ELISA Kit #7938, but does not affect the levels of total eIF4E detected by PathScan ® Total eIF4E Sandwich ELISA Kit #7940. HeLa cells (80-90% confluent) were serum-starved over night and then treated with 20% FBS at 37ºC. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using eIF4E Antibody #9742 (left panel) or Phospho-eIF4E Antibody (Ser209) #9741 (right panel) are shown in the bottom figure.

PathScan ® Total eIF4E Sandwich ELISA Kit - 7940 from Cell Signaling Technology

Category ELISA Kit

eukaryotic translation initiation factor 4E, ELISA Kit - MBS9330784 from MyBioSource

Category ELISA Kit
Prices $435.00, $600.00, $2,650.00, $5,205.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
Sensitivity 0.1ng/ml.
Range 0.625ng/ml-20ng/ml.

Human Phospho-eIF-4E (S209) ELISA - PEL-EIF4E-S209 from RayBiotech

Category ELISA Kit
Prices $481.00, $866.00, $1,924.00
Sizes 1 plate kit, 2 plate kit, 5 plate kit
eIF4E (S209)- Positive Control

Human eIF-4E (Phospho-S209) ELISA Kit - orb320101 from Biorbyt

Category ELISA Kit
Prices $918.00, $1,573.00, $3,366.00
Sizes 96 assays, 192 assays, 480 assays

Human Phospho-eIF-4E (S209) ELISA - PEL-EIF4E-S209-1 from RayBiotech

Category ELISA Kit
Prices $491.00, $883.80, $1,964.00
Sizes 1 plate kit, 2 plate kit, 5 plate kit
HeLa cells were treated with Anisomycin. Solubilize cells at 4 x 10^7 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA. Please see step 3 of Part VI Reagent Preparation for detail.

Phospho-eIF4E (Ser209) ELISA Kit - K4212 from Biovision

Category ELISA Kit
Prices $945.00
Sizes 100 assays

Human EIF4E ELISA Kit - E013770 from Biobool

Category ELISA Kit
Sensitivity 0.1ng/ml.

anti-eIF4E antibody - ARG51206 from Arigo Biolaboratories

Category ELISA Kit

anti-eIF4E antibody - ARG65706 from Arigo Biolaboratories

Category ELISA Kit

anti-eIF4E antibody - ARG55572 from Arigo Biolaboratories

Category ELISA Kit

eIF4E, ELISA Kit - MBS846688 from MyBioSource

Category ELISA Kit
Prices $1,095.00
Sizes 100 assays
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