SPAM1

Gene Symbol SPAM1
Entrez Gene 6677
Alt Symbol HEL-S-96n, HYA1, HYAL1, HYAL3, HYAL5, PH-20, PH20, SPAG15
Species Human
Gene Type protein-coding
Description sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
Other Description epididymis secretory sperm binding protein Li 96n|hyal-PH20|hyaluronidase PH-20|hyaluronoglucosaminidase PH-20|sperm surface protein PH-20
Swissprots P38567 Q8TC30
Accessions AAQ96882 CBH19453 CBL54474 CBL86562 CCA64576 CCD32726 CCF70987 EAL24329 EAL24330 EAW83604 EAW83605 EAW83606 P38567 AK292229 BAF84918 AY920278 AAX13985 AY920279 AAX13986 AY920280 AAX13987 AY920281 AAX13988 BC026163 AAH26163 BI831525 DB023094 DQ891657 ABM82583 DQ894844 ABM85770 EU794686 ACJ13740 L13779 L13781 S67798 AAC60607 X84347 CAA59086 XM_011516523 XP_011514825 XM_011516524 XP_011514826 XM_011516525 XP_011514827 XM_011516526 XP_011514828 NM_001174044 NP_001167515 NM_001174045 NP_001167516 NM_001174046 NP_001167517 NM_003117 NP_003108 NM_153189 NP_694859
Function Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. {ECO:0000269|PubMed:8282124}.
Subcellular Location Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Specificity Testis. {ECO:0000269|PubMed:8234258}.
Top Pathways Glycosaminoglycan degradation

SPAM1 (H-71) - sc-99024 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

SPAM1 (N-17) - sc-49202 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Hyaluronidase PH20 antibody - ab196596 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of Hela cells labelling Hyaluronidase PH20 using ab196596 at 1/50 (top) with DAPI blue for nuclear staining (bottom)

Anti-Hyaluronidase PH20 antibody - ab193009 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Hyaluronidase PH20 antibody (ab193009) at 1 µg/ml + 786-0 whole cell lysate at 10 µg

Anti-Hyaluronidase PH20 antibody - C-terminal - ab135314 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-Hyaluronidase PH20 antibody - C-terminal (ab135314) at 1 µg/ml + Jurkat cell lysate at 10 µg

Anti-SPAM1 / PH20 Antibody (aa437-486) - LS-C151669 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SPAM1 / PH20 antibody Western Blot of Human Placenta. Antibody Dilution: 1.0ug/ml. Antibody dilution: 1 ug/ml.

Anti-SPAM1 / PH20 Antibody - LS-C192906 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SPAM1 / PH20 Antibody (aa429-457, AP) - LS-C270096 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPAM1 / PH20 Antibody (aa429-457, Biotin) - LS-C270099 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPAM1 / PH20 Antibody (aa429-457, APC) - LS-C270097 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPAM1 / PH20 Antibody (aa429-457, FITC) - LS-C270100 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPAM1 / PH20 Antibody (aa429-457, Azide-free, HRP) - LS-C270101 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPAM1 / PH20 Antibody (aa429-457, PE) - LS-C270102 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPAM1 / PH20 Antibody (aa429-457) - LS-C325308 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPAM1 / PH20 Antibody (Internal) - LS-C345607 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of SPAM1 Antibody with human 786-0 Whole Cell lysate.

Anti-SPAM1 / PH20 Antibody - LS-C331909 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of HeLa cell using SPAM1 antibody. Blue: DAPI for nuclear staining.

SPAM1 Antibody - NBP1-81637 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: SPAM1 Antibody [NBP1-81637] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

SPAM1 Antibody - H00006677-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

ANTI-SPAM1(C-TERMINAL) antibody produced in rabbit - SAB1305240 from Sigma-Aldrich

Prices $326.00
Sizes 40 tst
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-SPAM1 (C-term): <B>Cat. No. SAB1305240</B>: SPAM1 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35 μg/lane).

Anti-SPAM1 antibody produced in rabbit - HPA017984 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-SPAM1: <B>Cat. No. HPA017984</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human testis shows distinct positivity in spermatids.

SPAM1 Antibody - C-terminal region (ARP64264_P050) - ARP64264_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SPAM1 Antibody - middle region (ARP64265_P050) - ARP64265_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SPAM1-Antibody-C-term - AP19001b from Abgent, a WuXi AppTec company

Host Rabbit
Clonality Polyclonal
SPAM1 Antibody (C-term) (Cat. #AP19001b) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SPAM1 antibody detected the SPAM1 protein (arrow).

Bovine SPAM1 Antibody - AF6436 from R&D Systems

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Detection of Bovine SPAM1 by Western Blot. Western blot shows lysates of recombinant bovine SPAM1 (Catalog # 6436-GH ). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Bovine SPAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6436) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). A specific band was detected for SPAM1 at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .

Bovine SPAM1 Affinity Purified Polyclonal Ab, Sheep IgG - AF6436-SP from R&D Systems

Host Sheep
Clonality Polyclonal
Detection of Bovine SPAM1 by Western Blot. Western blot shows lysates of recombinant bovine SPAM1 (Catalog # 6436-GH). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Bovine SPAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6436) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SPAM1 at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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