DKC1

Gene Symbol DKC1
Entrez Gene 1736
Alt Symbol CBF5, DKC, DKCX, NAP57, NOLA4, XAP101
Species Human
Gene Type protein-coding
Description dyskeratosis congenita 1, dyskerin
Other Description CBF5 homolog|H/ACA ribonucleoprotein complex subunit 4|nopp140-associated protein of 57 kDa|nucleolar protein NAP57|nucleolar protein family A member 4|snoRNP protein DKC1
Swissprots Q9Y505 O60832 Q96G67 F5BSB3 O43845
Accessions AAD20232 CAA11970 CAB39106 CAB51168 EAW72661 EAW72662 EAW72663 O60832 AF067008 AAD11815 AK225077 AK307672 AM392631 CAL37509 AM392823 CAL37701 BC009928 AAH09928 BC010015 AAH10015 BI858251 BU730947 CD367400 DA429806 DB517187 DQ892737 ABM83663 DQ895984 ABM86910 HY050100 JF279874 ADX66370 KC954523 AGZ20497 KC954524 AGZ20498 KC954525 AGZ20499 U59151 AAB94299 NM_001142463 NP_001135935 NM_001288747 NP_001275676 NM_001363 NP_001354 NR_110021 NR_110022 NR_110023
Function Isoform 1: Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
Subcellular Location Isoform 1: Nucleus, nucleolus. Nucleus, Cajal body. Note=Also localized to Cajal bodies (coiled bodies).
Tissue Specificity Ubiquitously expressed. {ECO:0000269|PubMed:10903840}.
Top Pathways Ribosome biogenesis in eukaryotes

Dyskerin (C-11) - sc-365731 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Dyskerin (C-11): sc-365731. Western blot analysis of Dyskerin expression in HeLa nuclear extract.

Dyskerin (C-15) - sc-26982 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dyskerin (C-15): sc-26982. Western blot analysis of Dyskerin expression in HeLa nuclear extract.

Dyskerin (H-3) - sc-373956 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Dyskerin (H-3): sc-373956. Western blot analysis of Dyskerin expression in HeLa nuclear extract.

Dyskerin (H-300) - sc-48794 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Dyskerin (H-300): sc-48794. Western blot analysis of Dyskerin expression in HeLa (A) and MCF7 (B) nuclear extracts.

Dyskerin (N-17) - sc-26980 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-DKC1 antibody - ab64667 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DKC1 antibody (ab64667) at 1 µg/mlLane 1 : HCT 116 (Human Colorectal Carcinoma) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 4 : Colon (Rat) Tissue LysateLane 5 : Thymus (Rat) Tissue LysateLane 6 : SW480 (Human colon adenocarcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-DKC1 antibody - ab174920 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-DKC1 antibody (ab174920) at 1/1000 dilution + Mouse testis tissue lysate at 35 µg

Anti-DKC1 antibody - ab126049 from Abcam

Prices $384.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DKC1 antibody (ab126049) at 1/1000 dilutionLane 1 : HeLa whole cell lysateLane 2 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-DKC1 antibody - ab60174 from Abcam

Prices $378.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
ab60174 at 5ug/ml staining DKC1 in human spleen tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

Anti-DKC1 antibody - ab64955 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DKC1 antibody (ab64955) at 1/500 dilutionLane 1 : HeLa cell extractLane 2 : HeLa cell extract with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-DKC1 antibody [EPR10398] - ab156006 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10398
All lanes : Anti-DKC1 antibody [EPR10398] (ab156006) at 1/1000 dilutionLane 1 : SW480 cell lysateLane 2 : MCF7 cell lysateLane 3 : HeLa cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilutionObserved band size : 58 kDa (why is the actual band size different from the predicted?)

Anti-DKC1 antibody [EPR10399] - ab156877 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10399
All lanes : Anti-DKC1 antibody [EPR10399] (ab156877) at 1/1000 dilutionLane 1 : SW480 cell lysateLane 2 : HeLa cell lysateLane 3 : MCF-7 cell lysateLysates/proteins at 10 µg per lane.developed using the ECL technique

Anti-DKC1 / Dyskerin Antibody (aa185-213, AP) - LS-C213555 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DKC1 / Dyskerin Antibody (aa185-213, APC) - LS-C213556 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DKC1 / Dyskerin Antibody (aa185-213, Biotin) - LS-C213557 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DKC1 / Dyskerin Antibody - LS-C192756 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts of various cell lines, using DKC1 antibody.

Anti-DKC1 / Dyskerin Antibody (aa185-213, FITC) - LS-C213558 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DKC1 / Dyskerin Antibody (aa35-84) - LS-C110511 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
DKC1 antibody LS-C110511 Western blot of Fetal Small Intestine lysate.

Anti-DKC1 / Dyskerin Antibody (aa185-213, PE) - LS-C213560 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DKC1 / Dyskerin Antibody (aa185-213, Azide-free, HRP) - LS-C213559 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DKC1 / Dyskerin Antibody - LS-C307888 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-DKC1 / Dyskerin Antibody (aa340-390) - LS-C289322 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-DKC1 / Dyskerin Antibody (aa390-440) - LS-C289324 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-DKC1 / Dyskerin Antibody (Lys203) - LS-C176706 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of Dyskerin (K203) pAb in extracts from HeLa cells.

Anti-DKC1 / Dyskerin Antibody (aa171-220) - LS-C118703 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of HeLa cells, using Dyskerin Antibody. The picture on the right is treated with the synthesized peptide.
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