PPP2R5A

Gene Symbol PPP2R5A
Entrez Gene 5525
Alt Symbol B56A, PR61A
Species Human
Gene Type protein-coding
Description protein phosphatase 2, regulatory subunit B', alpha
Other Description PP2A B subunit isoform B'-alpha|PP2A B subunit isoform B56-alpha|PP2A B subunit isoform PR61-alpha|PP2A B subunit isoform R5-alpha|PP2A, B subunit, B' alpha isoform|PP2A, B subunit, B56 alpha isoform|PP2A, B subunit, PR61 alpha isoform|PP2A, B subunit, R5 alpha isoform|PR61alpha|protein phosphatase 2, regulatory subunit B (B56), alpha isoform|protein phosphatase 2, regulatory subunit B', alpha isoform|serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, alpha isoform|serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform
Swissprots B7Z7L2 B2R6D2 Q8TBI9 D3DT99 Q15172 Q5VVB2 Q2NL72
Accessions EAW93392 EAW93393 Q15172 AA425592 AK302202 BAH13648 AK312530 BAG35429 BC022474 AAH22474 BC110883 AAI10884 BM974076 BP326450 DQ892361 ABM83287 DQ895568 ABM86494 L42373 AAC37601 NM_001199756 NP_001186685 NM_006243 NP_006234
Function The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:8703017}. Nucleus {ECO:0000269|PubMed:8703017}. Chromosome, centromere {ECO:0000269|PubMed:16541025}. Note=From mitotic prophase to metaphase, localizes at the inner centromere between a pair of sister kinetochores. Decreased expression at the onset of anaphase. {ECO:0000269|PubMed:16541025}.
Tissue Specificity Widely expressed with the highest expression in heart and skeletal muscle.
Top Pathways Oocyte meiosis, mRNA surveillance pathway, Dopaminergic synapse, PI3K-Akt signaling pathway, Adrenergic signaling in cardiomyocytes

PP2A-B56-α (F-10) - sc-271151 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PP2A-B56-α (F-10): sc-271151. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

PP2A-B56-α (F-4) - sc-271311 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PP2A-B56-α (F-4): sc-271311. Western blot analysis of PP2A-B56-α expression in non-transfected: sc-117752 (A) and human PP2A-B56-α transfected: sc-112024 (B) 293T whole cell lysates.

PP2A-B56-α (C-18) - sc-6116 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PP2A-B56-α (C-18): sc-6116. Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization.

PP2A-B56-α (23) - sc-136045 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
PP2A-B56-α (23): sc-136045. Immunofluorescence staining of rabbit brain cells showing nuclear and cytoplasmic localization.

Anti-PPP2R5A antibody - ab72028 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab72028 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab72028 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-PPP2R5A antibody - ab89621 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-PPP2R5A antibody (ab89621) at 1 µg/ml + Human placenta tissue lysate at 50 µg

Anti-PPP2R5A antibody - ab86551 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PPP2R5A antibody (ab86551) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-PPP2R5A antibody - ab137924 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PPP2R5A antibody (ab137924) at 1/500 dilution + HeLa cell lysate at 30 µg

Anti-PPP2R5A antibody - ab1084 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-PPP2R5A antibody - C-terminal - ab154678 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-PPP2R5A antibody - C-terminal (ab154678) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg

Anti-PPP2R5A Antibody (aa473-486) IHC-plus™ - LS-B10270 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-PPP2R5A antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-PPP2R5A Antibody (aa25-75) - LS-C91582 from LifeSpan Bioscience

Prices $545.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal

Anti-PPP2R5A Antibody (C-Terminus) - LS-C131256 from LifeSpan Bioscience

Host Goat
Clonality Polyclonal

Anti-PPP2R5A Antibody (aa418-446, APC) - LS-C207512 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PPP2R5A Antibody (aa418-446, AP) - LS-C207511 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PPP2R5A Antibody (aa418-446, Biotin) - LS-C207513 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PPP2R5A Antibody (aa418-446, FITC) - LS-C207514 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PPP2R5A Antibody (aa418-446, Azide-free, HRP) - LS-C207515 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PPP2R5A Antibody (aa418-446, PE) - LS-C207516 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PPP2R5A Antibody (aa304-353) - LS-C200518 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of the lysates from HeLa cells using PPP2R5A antibody.

Anti-PPP2R5A Antibody (aa363-408) - LS-C313892 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PPP2R5A Antibody (aa417-446) - LS-C324243 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PPP2R5A Antibody (aa107-156) - LS-C102281 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PPP2R5A antibody LS-C102281 Western blot of Transfected 293T cell lysate.

Anti-PPP2R5A Antibody (aa418-446) - LS-C162088 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
PPP2R5A Antibody western blot of mouse liver tissue lysates (35 ug/lane). The PPP2R5A antibody detected the PPP2R5A protein (arrow).

Anti-PPP2R5A Antibody (aa25-75) - LS-C287800 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse PPP2R5A by WB (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PPP2R5A antibody used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
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