ENO2

Gene Symbol ENO2
Entrez Gene 2026
Alt Symbol HEL-S-279, NSE
Species Human
Gene Type protein-coding
Description enolase 2 (gamma, neuronal)
Other Description 2-phospho-D-glycerate hydro-lyase|2-phospho-D-glycerate hydrolyase|epididymis secretory protein Li 279|gamma-enolase|neural enolase|neuron specific gamma enolase|neuron-specific enolase|neurone-specific enolase
Swissprots P09104 Q96J33 B7Z2X9
Accessions AAB51320 CAA36215 CBN61368 EAW88709 EAW88710 EAW88711 P09104 AK124656 BAG54062 AK290525 BAF83214 AK295220 BAH12015 AK312402 BAG35316 BC002745 AAH02745 BT007383 AAP36047 CR536582 CAG38819 DQ893819 ABM84745 EU176168 ABW03619 EU794607 ACJ13661 M22349 AAB59554 M36768 AAA52388 X13120 CAA31512 X14327 CAA32505 Y00691 NM_001975 NP_001966
Function Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm {ECO:0000250}. Cell membrane {ECO:0000250}. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. {ECO:0000250}.
Tissue Specificity The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Top Pathways Glycolysis / Gluconeogenesis, RNA degradation, Biosynthesis of amino acids, Carbon metabolism

Enolase-2 Antibody - 9536 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines and human and mouse brain using Enolase-2 Antibody.

Enolase-2 (D20H2) Rabbit mAb - 8171 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D20H2
Western blot analysis of extracts from various cell lines using Enolase-2 (D20H2) Rabbit mAb.

Enolase (5A4) - sc-51880 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Enolase (5A4): sc-51880. Western blot analysis of Enolase expression in IMR-32 (A), Hep G2 (B), A549 (C) and SH-SY5Y (D) whole cell lysates and mouse brain tissue extract (E).

γ Enolase (D-7) - sc-376375 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
γ Enolase (D-7): sc-376375. Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing neuropil and cytoplasmic staining of neuronal cells.

γ Enolase (NSE-P1) - sc-21738 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
γ Enolase (NSE-P1): sc-21738. Western blot analysis of γ Enolase expression in SH-SY-5Y whole cell lysate.

γ Enolase (N-14) - sc-31859 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
γ Enolase (N-14): sc-31859. Western blot analysis of γ Enolase expression in Y79 (A), U-87 MG (B) and SK-N-SH (C) whole cell lysates and rat pituitary (D), rat brain (E) and mouse brain (F) tissue extracts.

γ Enolase (T-12) - sc-31860 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
γ Enolase (T-12): sc-31860. Immunoperoxidase staining of formalin fixed, paraffin-embedded human hippocampus tissue showing cytoplasmic staining of glandular cells.

γ Enolase (NSE-P2) - sc-21737 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
γ Enolase (NSE-P2): sc-21737. Western blot analysis of γ Enolase expression in Hep G2 whole cell lysate.

γ Enolase (3F289) - sc-71047 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
γ Enolase (3F289): sc-71047. Western blot analysis of γEnolase expression in Hep G2 whole cell lysate.

γ Enolase (SPM347) - sc-56384 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
γ Enolase (SPM347): sc-56384. Western blot analysis of Enolase expression in IMR-32 (A), Hep G2 (B), SK-N-SH (C) and BE (2)-M17 (D) whole cell lysates.

γ Enolase (3F290) - sc-71046 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
γ Enolase (3F290): sc-71046. Western blot analysis of γ Enolase expression in non-transfected 293T: sc-117752 (A), human γ Enolase transfected 293T: sc-170262 (B) and Y79 (C) whole cell lysates.

Anti-NSE antibody - ab53025 from Abcam

Prices $403.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab53025 staining NSE in Rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3 Triton-X,  and blocked with 5% serum for 1 hour at room temperature. The sample was incubated with primary antibody (1/250) at 4°C for 16 hours. Ab96921, DyLight® 594-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.See Abreview

Anti-NSE antibody - ab39369 from Abcam

Prices $379.00
Sizes 100 µl
Host Chicken
Clonality Polyclonal
Dissociated cell cultures of neonatal mouse brains, showing NSE (green staining) in neurons.These cultures were counter-stained with a rabbit anti-GFAP to localize astrocytes, as well as with DAPI (blue staining) to localize nuclei.

Anti-NSE antibody - ab134015 from Abcam

Prices $384.00
Sizes 100 µg
Host Chicken
Clonality Polyclonal
ICC/IF image of ab134015 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab134015, 0.5µg/ml) overnight at +4°C. The secondary antibody (green) was ab46947, DyLight® 488 goat anti-chicken IgY (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-NSE antibody - ab128034 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NSE antibody (ab128034) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : HepG2 cell extract with synthesized peptide

Anti-NSE antibody - ab64721 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-NSE antibody (ab64721) at 1/2000 dilution + Rat spinal cord

Anti-NSE antibody - ab76682 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-NSE antibody (ab76682) at 1/50 dilution + Y79 cell line lysates at 35 µg

Anti-NSE antibody - ab89428 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-NSE antibody (ab89428) at 1 µg/ml + human stomach tissue lysate at 50 µg

Anti-NSE antibody - ab15486 from Abcam

Prices $376.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
ab15486 staining NSE in human pancreas by Immunohistochemistry (FFPE-sections).

Anti-NSE antibody - ab166817 from Abcam

Host Sheep
Clonality Polyclonal

Anti-NSE antibody [18H2] - ab21253 from Abcam

Prices $372.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 18H2

Anti-NSE antibody [1C1] - ab54201 from Abcam

Prices $359.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal
Clone 1C1

Anti-NSE antibody [1C1] - ab8328 from Abcam

Prices $370.00
Sizes 500 µg
Host Mouse
Clonality Monoclonal
Clone 1C1

Anti-NSE antibody [1C1] (HRP) - ab24472 from Abcam

Prices $379.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal
Clone 1C1
All lanes : Anti-NSE antibody [1C1] (HRP) (ab24472) at 1/1000 dilutionLane 2 : Human NSE full length protein (ab78797) at 5 µgLane 3 : Human NSE full length protein (ab78797) at 0.005 µgLane 4 : Whole tissue lysate prepared from human brain at 15 µgdeveloped using the ECL techniqueObserved band size : 45 kDa (why is the actual band size different from the predicted?)Exposure time : 1 minuteImage courtesy of Dr Rachel Barker by Abreview.See Abreview

Anti-NSE antibody [5A4] - ab10169 from Abcam

Prices $379.00
Sizes 250 µg
Host Mouse
Clonality Monoclonal
Clone 5A4
Ab10169 staining human normal pancreas. Staining is localized to cell membrane and cytoplasm.Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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