ATR

Gene Symbol ATR
Entrez Gene 545
Alt Symbol FCTCS, FRP1, MEC1, SCKL, SCKL1
Species Human
Gene Type protein-coding
Description ATR serine/threonine kinase
Other Description FRAP-related protein-1|MEC1, mitosis entry checkpoint 1, homolog|ataxia telangiectasia and Rad3-related protein|serine/threonine-protein kinase ATR
Swissprots Q13535 Q9BXK4 Q59HB2 Q7KYL3 Q93051
Accessions AAK26749 CAA03590 EAW78969 EAW78970 Q13535 AB208847 BAD92084 AK307402 BC172404 AAI72404 BE859077 DC346860 U49844 AAC50405 U76308 AAC50929 Y09077 CAA70298 XM_011512924 XP_011511226 XM_011512925 XP_011511227 XM_011512926 XP_011511228 XM_011512927 XP_011511229 XR_924147 XR_924148 XR_924149 NM_001184 NP_001175
Function Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. {ECO:0000269|PubMed:10597277, ECO:0000269|PubMed:10608806, ECO:0000269|PubMed:10859164, ECO:0000269|PubMed:11114888, ECO:0000269|PubMed:11418864, ECO:0000269|PubMed:11673449, ECO:0000269|PubMed:11721054, ECO:0000269|PubMed:11865061, ECO:0000269|PubMed:12526805, ECO:0000269|PubMed:127919
Subcellular Location Nucleus {ECO:0000269|PubMed:11114888, ECO:0000269|PubMed:11721054, ECO:0000269|PubMed:11865061, ECO:0000269|PubMed:12814551, ECO:0000269|PubMed:14871897, ECO:0000269|PubMed:16260606, ECO:0000269|PubMed:18283122, ECO:0000269|PubMed:8843195}. Nucleus, PML body {ECO:0000250}. Chromosome {ECO:0000250}. Note=Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
Tissue Specificity Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary. {ECO:0000269|PubMed:11470508, ECO:0000269|PubMed:8610130, ECO:0000269|PubMed:8843195}.
Top Pathways Cell cycle, p53 signaling pathway, HTLV-I infection, Fanconi anemia pathway

Phospho-ATR (Ser428) Antibody - 2853 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of Raw264.7, SV-T2 and HT-29 cells that were untreated or UV-treated (50 mJ, 30 min), using Phospho-ATR (Ser428) Antibody. Lambda phosphatase NEB #P0753 (10,000 Units/ml, 1hr) was used to demonstrate the phospho-specificity of the antibody.

ATR (E1S3S) Rabbit mAb - 13934 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone E1S3S
Western blot analysis of extracts from various cell lines using ATR (E1S3S) Rabbit mAb.

ATR Antibody - 2790 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using ATR Antibody.

ATR (N-19) - sc-1887 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ATR (N-19): sc-1887. Western blot analysis of ATR expression in HeLa (A), A-431 (B) and K-562 (C) whole cell lysates.

ATR (C-19) - sc-1888 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ATR (C-19): sc-1888. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.

ATR (C-20) - sc-21848 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ATR (C-20): sc-21848. Western blot analysis of ATR expression in A-431 whole cell lysate.

ATR (C-17) - sc-23737 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ATR (C-17): sc-23737. Western blot analysis of ATR expression in K-562 nuclear extract.

ATR (H-300) - sc-28901 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ATR (H-300): sc-28901. Western blot analysis of ATR expression in HeLa (A), A-431 (B) and ECV304 (C) whole cell lysates.

ATR (E-18) - sc-21849 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ATR (E-18): sc-21849. Western blot analysis of ATR expression in K-562 nuclear extract.

ATR (E-14) - sc-23736 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

p-ATR (Ser 428) - sc-109912 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-ATR (Ser 428): sc-109912. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

Anti-ATR (phospho S428) antibody [EPR2184] - ab178407 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2184
All lanes : Anti-ATR (phospho S428) antibody [EPR2184] (ab178407) at 1/1000 dilutionLane 1 : HeLa cell lysate untreatedLane 2 : HeLa cell lysate treated with Okadaic Acid + Calyculin ALysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-ATR antibody - ab10312 from Abcam

Prices $382.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ATR antibody - ab101900 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ATR antibody (ab101900) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with immunising peptide at 1 µg/mlLane 6 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate with immunising peptide at 1 µg/mlLane 7 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate with immunising peptide at 1 µg/mlLane 8 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate with immunising peptide at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-ATR antibody - ab10327 from Abcam

Prices $378.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ATR antibody (ab10327) at 0.07 µg/mlLane 1 : HeLa whole cell lysateLane 2 : U2OS whole cell lysateLane 3 : HeLa whole cell lysateLane 4 : U2OS whole cell lysateLysates/proteins at 20 µg per lane.developed using the ECL technique

Anti-ATR antibody - ab10946 from Abcam

Prices $359.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-ATR antibody - ab184137 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-ATR antibody (ab184137) at 1/500 dilution + A431 whole cell lysate at 30 µg

Anti-ATR antibody [2B5] - ab4471 from Abcam

Prices $382.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2B5

Anti-ATR antibody - ChIP Grade - ab2905 from Abcam

Prices $400.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ATR antibody - N-terminal - ab154326 from Abcam

Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labeling ATR with ab154326 at 1/500 dilution. Lower panel: Hoechst 33342 costaining.

Anti-ATR Antibody (aa2122-2644) - LS-C12026 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ATR Antibody (aa2122-2380) - LS-C12028 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ATR Antibody (aa400-460) - LS-C12029 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ATR Antibody (aa2122-2380) - LS-C42753 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ATR Antibody (aa2122-2644) - LS-C42685 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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