EMG1

Gene Symbol EMG1
Entrez Gene 10436
Alt Symbol C2F, Grcc2f, NEP1
Species Human
Gene Type protein-coding
Description EMG1 N1-specific pseudouridine methyltransferase
Other Description 18S rRNA (pseudouridine(1248)-N1)-methyltransferase|18S rRNA (pseudouridine-N1-)-methyltransferase NEP1|18S rRNA Psi1248 methyltransferase|EMG1 nucleolar protein homolog|essential for mitotic growth 1|ribosomal RNA small subunit methyltransferase NEP1|ribosome biogenesis protein NEP1
Swissprots O00726 Q92979 O00675
Accessions AAB51325 EAW88694 EAW88695 EAW88696 EAW88697 EAW88698 Q92979 AA314580 AI394112 AK291597 BAF84286 AK298207 BAG60477 AK298745 BAG60889 AK307177 BC055314 AAH55314 BM709304 BQ015307 CD704778 DA070200 DC403138 HQ447756 ADQ32241 U56427 U72514 AAC51641 XM_011520907 XP_011519209 NM_006331 NP_006322
Function S-adenosyl-L-methionine-dependent pseudouridine N(1)- methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position (PubMed:20047967). Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes (By similarity). {ECO:0000250|UniProtKB:Q06287, ECO:0000269|PubMed:20047967}.
Subcellular Location Nucleus, nucleolus {ECO:0000269|PubMed:11935223}.
Top Pathways Ribosome biogenesis in eukaryotes

EMG1 (D-13) - sc-132663 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
EMG1 (D-13): sc-132663. Western blot analysis of EMG1 expression in non-transfected: sc-117752 (A) and human EMG1 transfected: sc-370922 (B) 293T whole cell lysates.

EMG1 (E-16) - sc-101948 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
EMG1 (E-16): sc-101948. Western blot analysis of EMG1 expression in Jurkat whole cell lysate.

EMG1 (FL-244) - sc-367445 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

EMG1 (P-12) - sc-132664 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

EMG1 (S-12) - sc-132665 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-EMG1 antibody - ab107293 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-EMG1 antibody (ab107293) at 1/100 dilution + HeLa cell lysate at 35 µg

Anti-EMG1 antibody - ab70509 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-EMG1 antibody (ab70509) at 1/500 dilutionLane 1 : EMG1 transfected 293T lysateLane 2 : Non transfected 293T lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-EMG1 / NEP1 Antibody (aa111-140, AP) - LS-C208879 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-EMG1 / NEP1 Antibody (aa111-140, APC) - LS-C208880 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-EMG1 / NEP1 Antibody (aa111-140, Biotin) - LS-C208881 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-EMG1 / NEP1 Antibody (aa111-140, FITC) - LS-C208882 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-EMG1 / NEP1 Antibody (aa111-140, Azide-free, HRP) - LS-C208883 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-EMG1 / NEP1 Antibody (aa51-100) - LS-C30367 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
EMG1 antibody LS-C30367 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.

Anti-EMG1 / NEP1 Antibody (aa111-140, PE) - LS-C208884 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-EMG1 / NEP1 Antibody (C-Terminus) - LS-C201388 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Human THP-1. EMG1 antibody dilution 1.0 ug/ml.

Anti-EMG1 / NEP1 Antibody (aa111-140) - LS-C329495 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-EMG1 / NEP1 Antibody - LS-C307394 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-EMG1 / NEP1 Antibody (aa104-133) - LS-C167747 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
EMG1 Antibody western blot of HeLa cell line lysates (35 ug/lane). The EMG1 antibody detected the EMG1 protein (arrow).

Anti-EMG1 / NEP1 Antibody - LS-C333311 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using EMG1 antibody.

EMG1 Antibody (1B8) - NBP2-02575 from Novus Biologicals

Prices $379.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1B8
Western Blot: EMG1 Antibody (1B8) [NBP2-02575] Analysis of extracts (35ug) from 9 different cell lines by using anti-EMG1 monoclonal antibody.

EMG1 Antibody - NBP1-57410 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: EMG1 Antibody [NBP1-57410] - HepG2 cell lysate, Antibody Titration: 0.2-1 ug/ml

EMG1 Antibody - H00010436-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: EMG1 Antibody [H00010436-B01P] - Analysis of EMG1 expression in transfected 293T cell line by EMG1 polyclonal antibody. Lane 1: EMG1 transfected lysate(26.84 KDa). Lane 2: Non-transfected lysate.

Monoclonal Anti-EMG1 antibody produced in mouse - SAB4100271 from Sigma-Aldrich

Prices $339.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone EMG1-A243
<B>Immunofluorescence</B><BR/>HeLa cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with Mouse Anti-EMG1 Clone: EMG1-A243 (<B>Cat. No. SAB4100271</B>) diluted to 1:200. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.

Anti-EMG1 antibody produced in rabbit - AV40636 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-EMG1: <B>Cat. No. AV40636</B>: Immunohistochemistry of EMG1 in Human Kidney tissue with EMG1 antibody at 4-8 μg/mL.

Anti-EMG1 antibody produced in mouse - SAB1406991 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-EMG1: <B>Cat. No. SAB1406991</B>: Antibody concentration: 10 μg/mL using HeLa cell.
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