Zap70

Gene Symbol Zap70
Entrez Gene 22637
Alt Symbol Srk, ZAP-70, mrtle, mur
Species Mouse
Gene Type protein-coding
Description zeta-chain (TCR) associated protein kinase
Other Description 70 kDa zeta-chain associated protein|syk-related tyrosine kinase|tyrosine-protein kinase ZAP-70
Swissprots P97455 Q80VV2 Q8CHJ3 P43404
Accessions EDL14509 EDL14510 P43404 AB083210 BAC43746 AB084383 BAC67015 AK039883 BAC30471 AK089056 BC029727 AAH29727 BY589032 CJ143180 U04379 AAA19250 U77667 AAB36538 XM_006495896 XP_006495959 NM_001289612 NP_001276541 NM_001289765 NP_001276694 NM_001289766 NP_001276695 NM_009539 NP_033565
Function Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion
Subcellular Location Cytoplasm. Cell membrane; Peripheral membrane protein. Note=In quiescent T-lymphocytes, ZAP70 is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
Tissue Specificity Isoform 1 and isoform 2 are expressed in thymus, spleen and lymph nodes. {ECO:0000269|PubMed:14985102}.
Top Pathways Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, Primary immunodeficiency

Anti-ZAP70 antibody produced in rabbit - SAB2107611 from Sigma-Aldrich

Prices $341.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-ZAP70: <B>Cat. No. SAB2107611</B>: Western blot analysis of Zap70 with Zap70 (zeta-chain, TCR, associated protein kinase) antibody against the middle region of Zap70 (50 μg) antibody at 1.0 μg/mL.

Anti-Zap-70 (11H40) Antibody (OAED00015) - OAED00015 from Aviva Systems Biology

Prices $310.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 11H40

Zap70 antibody - middle region (ARP61185_P050) - ARP61185_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Mouse-Zap70-Antibody-C-term - AP16081b from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Mouse Zap70 Antibody (C-term) (Cat. #AP16081b) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Zap70 antibody detected the Zap70 protein (arrow).

Zap70-Antibody - AM8462b from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Mouse
Clonality Monoclonal
Clone 1484CT290.68.62
All lanes : Anti-Zap70 Antibody at 1:2000 dilution Lane 1: Jurkat whole cell lysates Lane 2: MOLT-4 whole cell lysates Lane 3: mouse thymus lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 70 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Mouse Zap70 Antibody - C-terminal region (OAAB11481) - OAAB11481 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

ZAP70 antibody - orb32253 from Biorbyt

Prices $187.00, $383.00
Sizes 80 μl (Trial size), 400 μl
Host Rabbit
Clonality Polyclonal

Zap70 antibody (Tyr493) - 70R-37237 from Fitzgerald

Prices $295.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

ZAP70 antibody (Tyr493) - 70R-32566 from Fitzgerald

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

ZAP70 antibody - 70R-35228 from Fitzgerald

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Zap70 antibody (Tyr319) - 70R-37236 from Fitzgerald

Prices $295.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

ZAP70 antibody (Tyr319) - 70R-32565 from Fitzgerald

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

zAP70 Antibody - abx104321 from Abbexa

Host Rabbit
Clonality Polyclonal

PE anti-human/mouse ZAP-70 Antibody - 313403 from Biolegend

Prices $95.00, $210.00
Sizes 25 tests, 100 tests
Host Mouse
Clone 1E7.2
Human peripheral blood lymphocytes intracellular stained with CD4 FITC and 1E7.2 PE

Purified anti-human/mouse ZAP-70 Antibody - 313402 from Biolegend

Prices $155.00
Sizes 100 µg
Host Mouse
Clone 1E7.2
Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-ZAP-70 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

FITC anti-human/mouse ZAP-70 Antibody - 313406 from Biolegend

Prices $175.00
Sizes 100 tests
Host Mouse
Clone 1E7.2
Human peripheral blood lymphocytes intracellularly stained with 1E7.2 FITC and CD3 APC

Anti-ZAP70 antibody - STJ72750 from St John's Laboratory Ltd

Host Goat
Clonality Polyclonal

ZAP 70, Polyclonal Antibody - MBS629798 from MyBioSource

Prices $520.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

ZAP 70, Polyclonal Antibody - MBS625718 from MyBioSource

Prices $620.00
Sizes 10 blots
Host Rabbit
Clonality Polyclonal

ZAP 70, Polyclonal Antibody - MBS630330 from MyBioSource

Prices $620.00
Sizes 10 blots
Host Rabbit
Clonality Polyclonal

ZAP 70, Polyclonal Antibody - MBS629312 from MyBioSource

Prices $410.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

ZAP 70, Polyclonal Antibody - MBS624732 from MyBioSource

Prices $580.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

ZAP 70, Polyclonal Antibody - MBS625553 from MyBioSource

Prices $410.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

ZAP 70, aa326-341 - MBS644921 from MyBioSource

Clonality Polyclonal

ZAP 70 - MBS633462 from MyBioSource

Clonality Monoclonal
Clone 9E649
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