GLI3

Gene Symbol GLI3
Entrez Gene 2737
Alt Symbol ACLS, GCPS, GLI3-190, GLI3FL, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV
Species Human
Gene Type protein-coding
Description GLI family zinc finger 3
Other Description GLI-Kruppel family member GLI3|glioma-associated oncogene family zinc finger 3|oncogene GLI3|transcriptional activator GLI3|zinc finger protein GLI3
Swissprots A4D1W1 Q75MU9 Q9UDT5 O75219 Q9UJ39 P10071 Q17RW4 Q75MT0
Accessions AAP21869 AAS01998 AAS02015 CAB59315 EAL24002 EAW94144 EAW94145 P10071 AA330409 AI863961 AK299299 BAG61313 AK308429 BC032660 BC113616 AAI13617 BC117168 AAI17169 BF194991 BI497308 DA713549 M57609 AAA52564 XM_005249703 XP_005249760 XM_005249704 XP_005249761 XM_011515272 XP_011513574 XM_011515273 XP_011513575 XM_011515274 XP_011513576 NM_000168 NP_000159
Function Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'. {ECO:0000269|PubMed:10693759, ECO:0000269|PubMed:11238441, ECO:0000269|PubMed:17764085}.
Subcellular Location Nucleus. Cytoplasm. Cell projection, cilium. Note=GLI3FL is localized predominantly in the cytoplasm while GLI3R resides mainly in the nucleus. Ciliary accumulation requires the presence of KIF7 and SMO. Translocation to the nucleus is promoted by interaction with ZIC1.
Tissue Specificity Is expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium.
Top Pathways Hedgehog signaling pathway, Basal cell carcinoma, cAMP signaling pathway

GLI-3 (B-4) - sc-74478 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GLI-3 (B-4): sc-74478. Western blot analysis of GLI-3 expression in Y79 (A) and K-562 (B) nuclear extracts.

GLI-3 (C-20) - sc-6154 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GLI-3 (C-20): sc-6154. Western blot analysis of GLI-3 expression in K-562 (A), Jurkat (B), Y79 (C) and SK-NMC (D) whole cell lysates.

GLI-3 (H-280) - sc-20688 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of GLI-3 expression in P 23 whole cell lysate (A,B). Antibodies tested include GLI-3 (N-19): sc-6155 (A) and GLI-3 (H-280): sc-20688 (B).

GLI-3 (N-19) - sc-6155 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GLI-3 (N-19): sc-6155. Immunofluorescence staining of methanol-fixed SW480 cells showing nuclear and cytoplasmic localization.

Anti-Gli3 antibody - ab55437 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab55437 Gli3 antibody at 1ug/ml.

Anti-Gli3 antibody [EPR4594] - ab181130 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4594
Immunofluorescence analysis of 293 cells (fixative 4% paraformaldehyde) labeling Gli3 with ab181130 at a 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor 488™) secondary used at a 1/200 diution.

Anti-GLI3 Antibody (aa41-57) IHC-plus™ - LS-B73 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-GLI3 antibody IHC of human brain-glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B73 concentration 1.25 ug/ml.

Anti-GLI3 Antibody (aa303-561, Biotin) - LS-C297582 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GLI3 Antibody (aa875-925) - LS-C290097 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-GLI3 Antibody (aa111-343, FITC) - LS-C302955 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GLI3 Antibody (aa111-343, Biotin) - LS-C301049 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GLI3 Antibody (aa303-561, FITC) - LS-C303531 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GLI3 Antibody (aa11-60) - LS-C199001 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human brain tissue, using GLI-3 Antibody. The sample on the right was incubated with synthetic peptide.

Anti-GLI3 Antibody (aa303-561) - LS-C294190 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant GLI3.

Anti-GLI3 Antibody (aa111-343) - LS-C294189 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant GLI3.

Anti-GLI3 Antibody (clone 2C9) - LS-C197308 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2C9
Immunofluorescence of monoclonal antibody to GLI3 on HeLa cell . [antibody concentration 10 ug/ml]

Anti-GLI3 Antibody (aa40-57) - LS-C312915 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
WB of GLI3 antibody. All lanes: Anti-GLI3 at 0.5ug/ml. Lane 1: Rat Testis Tissue Lysate at 40ug. Lane 2: A549 Whole Cell Lysate at 40ug. Lane 3: SW620 Whole Cell Lysate at 40ug. Predicted bind size: 170KD. Observed bind size: 80KD.

Anti-GLI3 Antibody (aa1-479) - LS-C150112 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
GLI-3 in HeLa Human Cell Line. GLI-3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human/Mouse GLI-3 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (yellow) and counterstained with DAPI (blue).

Anti-GLI3 Antibody (aa431-442) - LS-C54916 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-GLI3 Antibody - LS-C36438 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-GLI3 Antibody (aa1000-1100) - LS-C209969 from LifeSpan Bioscience

Prices $295.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal

Anti-GLI3 Antibody (aa975-1025) - LS-C290096 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GLI-3 Antibody - NBP2-29627 from Novus Biologicals

Prices $99.00, $229.00
Sizes 25 µg, 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: GLI-3 Antibody [NBP2-29627] - WB analysis of GLI-3 in Hela cell lysate and MEF cell lysate.

GLI-3 Antibody - AF3690 from Novus Biologicals

Prices $99.00, $365.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal
Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse GLI‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for GLI‑3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

GLI-3 Antibody - NBP2-16665 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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