EGFR

Gene Symbol EGFR
Entrez Gene 1956
Alt Symbol ERBB, ERBB1, HER1, NISBD2, PIG61, mENA
Species Human
Gene Type protein-coding
Description epidermal growth factor receptor
Other Description avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|erb-b2 receptor tyrosine kinase 1|proto-oncogene c-ErbB-1|receptor tyrosine-protein kinase erbB-1
Swissprots Q9H3C9 Q9H2C9 Q9UMD7 Q9UMG5 Q14225 Q9UMD8 O00732 O00688 Q68GS5 P00533 Q92795 Q9BZS2 P06268 Q9GZX1
Accessions AAA52370 AAA63171 AAC50796 AAC50797 AAC50798 AAC50799 AAC50800 AAC50801 AAC50803 AAC50804 AAG35786 AAG35787 AAG35788 AAG35789 AAG35790 AAG43243 AAG43244 AAG43245 AAS07524 AAS83109 AFK93471 AFK93472 AFK93473 AFK93474 AFK93475 AFK93476 AFK93477 AFK93478 AIE16170 AIE16171 BAE15958 BAF79636 BAF80003 BAF80004 BAL40868 BAL41217 CAA29668 CAA34902 CAJ55813 CAM91181 CAV33298 CBK51921 CBK51922 CBK51923 CBK51924 CCO13722 CCO75437 CCO75439 CCV20014 EAW50962 EAW50963 EAW50964 EAW50965 P00533 AB209442 BAD92679 AF125253 AAG43240 AF277897 AAK01080 AI217671 AK127817 AK225422 AK290352 BAF83041 AK294750 BAH11869 AL598260 AU137334 AW163038 AW295229 AY550966 AAT52212 AY698023 AAT97978 AY698024 AAT97979 BC070081 BC094761 AAH94761 BC118665 AAI18666 BC128419 AAI28420 CB160831 DQ088980 AAZ66620 EF210210 ABQ66237 EF210211 ABQ66238 GU228573 GU228584 GU255993 ADL28125 HM437234 ADN43066 HM437235 HQ730875 HQ912715 ADZ75461 HQ999997 ADY76965 K03193 AAA52371 U48722 AAC50802 U51732 U95089 AAB53063 X00588 CAA25240 X00663 CAA25282 NM_005228 NP_005219 NM_201282 NP_958439 NM_201283 NP_958440 NM_201284 NP_958441
Function Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-ca
Subcellular Location Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
Tissue Specificity Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. {ECO:0000269|PubMed:17671655}.
Top Pathways Estrogen signaling pathway, Oxytocin signaling pathway, MicroRNAs in cancer, Proteoglycans in cancer, Central carbon metabolism in cancer

Phospho-EGF Receptor (Tyr1068) (D7A5) XP ® Rabbit mAb (Biotinylated) - 8543 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D7A5
Western blot analysis of extracts from A-431 cells, untreated (-) or treated with hEGF #8916 (100 ng/ml for 5 min) (+), using Phospho-EGF Receptor (Tyr1068) (D7A5) XP ® Rabbit mAb (Biotinylated).

Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb (Biotinylated) - 4031 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 1H12
Western blot analysis of extracts from A431 and COS cells, untreated or EGF-stimulated (100 ng/ml), using Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb (Biotinylated) (upper) detected using Stretavidin-HRP #3999 or EGF Receptor (D38B1) XP ® Rabbit mAb #4267 (lower).

EGF Receptor (D38B1) XP ® Rabbit mAb (PE Conjugate) - 8839 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D38B1
Flow cytometric analysis of Jurkat (blue) and HeLa cells (green) using EGF Receptor (D38B1) XP ® Rabbit mAb (PE Conjugate).

EGF Receptor (D38B1) XP ® Rabbit mAb (Biotinylated) - 6627 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D38B1
Western blot analysis of extracts from HeLa and A-431 cells using EGF Receptor (D38B1) XP ® Rabbit mAb (Biotinylated) and developed using Streptavidin-HRP #3999.

EGF Receptor (D38B1) XP ® Rabbit mAb (HRP Conjugate) - 8504 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D38B1
Western blot analysis of extracts from HeLa and A-431 cells using EGF Receptor (D38B1) XP ® Rabbit mAb (HRP Conjugate).

EGF Receptor (D38B1) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 5588 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D38B1
Confocal immunofluorescent analysis of A549 cells, serum-starved (left) or EGF-treated (#8916, right), using EGF Receptor (D38B1) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate) (blue pseudocolor) and Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (Alexa Fluor ® 488 Conjugate) #9683 (green).

EGF Receptor (E746-A750del Specific) (D6B6) XP ® Rabbit mAb (Biotinylated) - 5747 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D6B6
Western blot analysis of extracts from H3255 and HCC827 cells using EGF Receptor (E746-A750del Specific) (D6B6) XP ® Rabbit mAb (Biotinylated).

EGF Receptor (D38B1) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 5616 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D38B1
Confocal immunofluorescent analysis of A549 cells, serum-starved (left) or EGF-treated #8916 (right), using EGF Receptor (D38B1) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) (green). Blue psuedocolor= DRAQ5 ® #4084 (fluorescent nuclear dye).

EGF Receptor (D38B1) XP ® Rabbit mAb (Alexa Fluor ® 555 Conjugate) - 5108 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D38B1
Confocal immunofluorescent analysis of A549 cells, serum-starved (left) or EGF-treated (#8916, right), using EGF Receptor (D28B1) XP ® Rabbit mAb (Alexa Fluor ® 555 Conjugate) (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

EGF Receptor (D38B1) XP ® Rabbit mAb (Alexa Fluor ® 594 Conjugate) - 8742 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D38B1
Immunofluorescent analysis of A549 cells, untreated (left) or treated with Human Epidermal Growth Factor (hEGF) #8916 (100 ng/ml, 15 min; +) (right), using EGF Receptor (D38B1) XP ® Rabbit mAb (Alexa Fluor ® 594 Conjugate) (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

EGF Receptor (D38B1) XP ® Rabbit mAb (Pacific Blue™ Conjugate) - 12699 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D38B1
Flow cytometric analysis of Jurkat (blue) and HeLa (green) cells, using EGF Receptor (D38B1) XP ® Rabbit mAb (Pacific Blue™ Conjugate).

EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb (Biotinylated) - 5354 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 43B2
Western blot analysis of extracts from HCC827 and H3255 cells using EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb (Biotinylated) and detected with Strepavidin-HRP #3999.

Phospho-EGF Receptor (Tyr1068) (D7A5) XP ® Rabbit mAb - 3777 from Cell Signaling Technology

Prices $119.00, $318.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D7A5
Western blot analysis of extracts of BxPC-3 cells, untreated or EGF-stimulated, using Phospho-EGF Receptor (Tyr1068) (D7A5) XP ® Rabbit mAb (upper) and EGF Receptor Antibody #2232 (lower).

Phospho-EGF Receptor (Tyr998) (C24A5) Rabbit mAb - 2641 from Cell Signaling Technology

Prices $297.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C24A5
Western blot analysis of extracts from A431 and BxPC-3 cells, untreated or treated with EGF, using Phospho-EGF Receptor (Tyr998) (C24A5) Rabbit mAb (upper) and EGF Receptor Antibody #2232 (lower).

Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb - 2236 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Mouse
Clonality Monoclonal
Clone 1H12
Western blot analysis of extracts from A431 cells, untreated or EGF-stimulated (100 ng/ml), using Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb (upper) or EGF Receptor (C74B9) Rabbit mAb #2646 (lower).

Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb - 4407 from Cell Signaling Technology

Prices $307.00, $719.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Monoclonal
Clone 53A5
Western blot analysis of extracts from BxPc-3 cells treated with EGF (100 ng/ml for 5 min) or EGF plus the EGF receptor tyrosine kinase inhibitor Gefitinib (10 uM for 2 hrs), using Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb (upper) or EGF Receptor Antibody #2232 (lower).

Phospho-EGF Receptor (Tyr1045) Antibody - 2237 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from A431 cells, untreated or EGF-stimulated (100 ng/ml), using Phospho-EGF Receptor (Tyr1045) Antibody (upper) or EGF Receptor (C74B9) Rabbit mAb #2646 (lower).

Phospho-EGF Receptor (Tyr1068) Antibody - 2234 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of MDA-468 cells untreated and treated with EGF, using Phospho-EGF Receptor (Tyr1068) Antibody.

Phospho-EGF Receptor (Tyr845) Antibody - 2231 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from A431 cells, untreated or EGF-stimulated (100 ng/ml), using Phospho-EGF Receptor (Tyr845) Antibody (upper) or EGF Receptor (C74B9) Rabbit mAb #2646 (lower).

Phospho-EGF Receptor (Tyr1148) Antibody - 4404 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from A431 cells, untreated or EGF-stimulated, using Phospho-EGF Receptor (Tyr1148) Antibody (upper) or EGF Receptor Antibody #2232 (lower).

Phospho-EGF Receptor (Ser1046/1047) Antibody - 2238 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from A431 cells, untreated or stimulated with EGF (100 ng/ml, 10 min), using Phospho-EGF Receptor (Ser1046/1047) Antibody (upper) or EGF Antibody #2232 (lower). Calf intestinal phosphatase (CIP) treatment abolished the immunoreactivity of Phospho-EGF Receptor (Ser1046/1047) Antibody with EGF-stimulated A431 cell lysates (middle).

Phospho-EGF Receptor (Tyr992) Antibody - 2235 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from A431 cells, untreated or EGF-treated (100 ng/ml), using Phospho-EGF Receptor (Tyr992) Antibody (upper) or EGF Receptor Antibody #2232 (lower).

Phospho-EGF Receptor (Thr669) (D2F1) Rabbit mAb - 8808 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D2F1
Western blot analysis of extracts from A-431 cells serum starved overnight, and left untreated (-) or treated (+) with EGF (100 ng/ml, 15 min), using Phospho-EGF Receptor (Thr669) (D2F1) Rabbit mAb (upper) and EGF Receptor (D38B1) XP ® Rabbit mAb #4267 (lower).

Phospho-HER2/ErbB2 (Tyr1248)/EGFR (Tyr1173) Antibody - 2244 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from untreated SKBR3 and MCF7 cells, untreated or treated with neuregulin (NRG), using Phospho-HER2/ErbB2 (Tyr1248)/EGFR (Tyr1173) Antibody (upper) and HER2/ErbB2 Antibody #2242 (lower).

Phospho-EGF Receptor (Tyr1086) Antibody - 2220 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of cell extracts from A431 cells, untreated or EGF-treated, using Phospho-EGF Receptor (Tyr1086) Antibody (upper), and EGF Receptor Antibody #2232 (lower).
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